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Invitrogen
INSIG1 Recombinant Rabbit Monoclonal Antibody (0Q2B5)
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INSIG1 Antibody (MA5-55473) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Rat liver using INSIG1 Recombinant Monoclonal Antibody (Product # MA5-55473) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6. 0) prior to IHC staining.
Product Details
MA5-55473
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
0Q2B5
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 177-277 of human INSIG1.
Conjugate
Form
Liquid
Concentration
1.19 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
AB_3667713
Product Specific Information
Immunogen Sequence: ASAKLDFANN VQLSLTLAAL SLGLWWTFDR SRSGLGLGIT IAFLATLITQ FLVYNGVYQY TSPDFLYIRS WLPCIFFSGG VTVGNIGRQL AMGVPEKPHS D
Target Information
Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element-binding protein (SREBP) mediated signaling pathway. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: growth response protein (CL-6); Immediate-early protein CL-6; INSIG-1; INSIG-1 membrane protein; Insulin-induced gene 1 protein; Insulin-induced growth response protein CL-6; MGC1405
Gene Aliases: 1810013C12Rik; Cl-6; Insig-1; Insig1
UniProt ID: (Mouse) Q8BGI3, (Rat) Q08755
Entrez Gene ID: (Mouse) 231070, (Rat) 64194
lipid metabolic process
triglyceride metabolic process
cholesterol biosynthetic process
response to sterol depletion
steroid metabolic process
cholesterol metabolic process
negative regulation of steroid biosynthetic process
sterol biosynthetic process
SREBP signaling pathway
inner ear morphogenesis
middle ear morphogenesis
negative regulation of fat cell differentiation
negative regulation of fatty acid biosynthetic process
palate development
cranial suture morphogenesis
negative regulation of cargo loading into COPII-coated vesicle
response to estradiol
cellular response to insulin stimulus
response to lipid
response to peptide hormone
response to fatty acid
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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