Proteintech

INTS10 Polyclonal Antibody

FIGURE: 1 / 5

INTS10 Antibody (15271-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 15271-1-AP) INTS10 antibody at dilution of 1:3,000 incubated at room temperature for 1.5 hours.

INTS10 Antibody in Immunoprecipitation (IP)

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Product Details

15271-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 9 publications 9 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

INTS10 Fusion Protein Ag7383 (37-382 aa encoded by BC001915)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LLKVTEQCF NTLERSEMLL LLLRRFPETV VQHGVGLGEA LLEAETIEEQ ESPVNCFRKL FVCDVLPLII NNHDVRLPAN LLYKYLNKAA EFYINYVTRS TQIENQHQGA QDTSDLMSPS KRSSQKYIIE GLTEKSSQIV DPWERLFKIL NVVGMRCEWQ MDKGRRSYGD ILHRMKDLCR YMNNFDSEAH AKYKNQVVYS TMLVFFKNAF QYVNSIQPSL FQGPNAPSQV PLVLLEDVSN VYGDVEIDRN KHIHKKRKLA EGREKTMSSD DEDCSAKGRN RHIVVNKAEL ANSTEVLESF KLARESWELL YSLEFLDKEF TRICLAWKTD TWLWLRIFLT DMIIYQG (37-382 aa encoded by BC001915)

Target Information

INTS10 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A)and mediates 3-prime end processing of small nuclear RNAs U1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII.

Molecular cell

Rambout X,Cho H,Blanc R,Lyu Q,Miano JM,Chakkalakal JV,Nelson GM,Yalamanchili HK,Adelman K,Maquat LE

Thu Jan 19 00:00:00 UTC 2023

A combinatorial approach to uncover an additional Integrator subunit.

Cell reports

Offley SR,Pfleiderer MM,Zucco A,Fraudeau A,Welsh SA,Razew M,Galej WP,Gardini A

Tue Mar 28 00:00:00 UTC 2023

INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex.

Nature communications

Mascibroda LG,Shboul M,Elrod ND,Colleaux L,Hamamy H,Huang KL,Peart N,Singh MK,Lee H,Merriman B,Jodoin JN,Sitaram P,Lee LA,Fathalla R,Al-Rawashdeh B,Ababneh O,El-Khateeb M,Escande-Beillard N,Nelson SF,Wu Y,Tong L,Kenney LJ,Roy S,Russell WK,Amiel J,Reversade B,Wagner EJ

Thu Oct 13 00:00:00 UTC 2022

Integrator endonuclease drives promoter-proximal termination at all RNA polymerase II-transcribed loci.

Molecular cell

Stein CB,Field AR,Mimoso CA,Zhao C,Huang KL,Wagner EJ,Adelman K

Thu Nov 17 00:00:00 UTC 2022

Integrator Recruits Protein Phosphatase 2A to Prevent Pause Release and Facilitate Transcription Termination.

Molecular cell

Huang KL,Jee D,Stein CB,Elrod ND,Henriques T,Mascibroda LG,Baillat D,Russell WK,Adelman K,Wagner EJ

Thu Oct 15 00:00:00 UTC 2020

INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module.

Nature communications

Sabath K,Stäubli ML,Marti S,Leitner A,Moes M,Jonas S

Thu Jul 09 00:00:00 UTC 2020

Molecular basis for the interaction between Integrator subunits IntS9 and IntS11 and its functional importance.

Proceedings of the National Academy of Sciences of the United States of America

Wu Y,Albrecht TR,Baillat D,Wagner EJ,Tong L

Tue Apr 25 00:00:00 UTC 2017

CRISPR-Cas9 mediated genetic engineering for the purification of the endogenous integrator complex from mammalian cells.

Protein expression and purification

Baillat D,Russell WK,Wagner EJ

Thu Dec 01 00:00:00 UTC 2016

CRISPR-Cas9 mediated genetic engineering for the purification of the endogenous integrator complex from mammalian cells.

Protein expression and purification

Baillat D,Russell WK,Wagner EJ

Thu Dec 01 00:00:00 UTC 2016

DSIF and NELF interact with Integrator to specify the correct post-transcriptional fate of snRNA genes.

Nature communications

Yamamoto J,Hagiwara Y,Chiba K,Isobe T,Narita T,Handa H,Yamaguchi Y

Fri Jun 27 00:00:00 UTC 2014

DSIF and NELF interact with Integrator to specify the correct post-transcriptional fate of snRNA genes.

Nature communications

Yamamoto J,Hagiwara Y,Chiba K,Isobe T,Narita T,Handa H,Yamaguchi Y

Fri Jun 27 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Int10; Integrator complex subunit 10

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Gene Aliases: 4921521J11Rik; AI462004; C8orf35; INT10; INTS10; RGD1304711

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UniProt ID: (Human) Q9NVR2, (Mouse) Q8K2A7

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Entrez Gene ID: (Human) 55174, (Mouse) 70885, (Rat) 290679

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Function(s)

protein binding molecular_function

Process(es)

snRNA processing snRNA transcription from RNA polymerase II promoter

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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INTS10 Polyclonal Antibody

INTS10 Antibody (15271-1-AP) in WB

Product Details

15271-1-AP

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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