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IPAB Antibody (PA5-144414) in WB
Western blot analysis of IPAB in recombinant protein using a dilution of 1:2,000. Samples were incubated with IPAB polyclonal antibody (Product # PA5-144414). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 40.2 kDa. Observed band size: 43 kDa.
Product Details
PA5-144414
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Shigella flexneri IPAB protein (1-312aa)
Conjugate
Form
Liquid
Concentration
3.904 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3091661
Target Information
Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:10545510, PubMed:17367391). IpaB/SctE and IpaC/SctB are inserted into the host membrane where they form a pore and allow the translocation of effector proteins into the cytosol of target cells (PubMed:10545510, PubMed:17367391, PubMed:34809452). Interaction with IpaD/SctA at needle tips leads to the formation of the MxiH/SctF-IpaD/SctA-IpaB/SctE ternary complex, which is essential for host cell sensing (PubMed:17367391, PubMed:19433542). Interaction of IpaB/SctE with host membrane lipids promotes recruitment of IpaC/SctB at the needle tip concomitant with translocon insertion into the host membrane and type III secretion induction (PubMed:19433542).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ipaB
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