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IPAD Antibody (PA5-144413) in WB
Western blot analysis of IPAD in recombinant protein using a dilution of 1:2,000. Samples were incubated with IPAD polyclonal antibody (Product # PA5-144413). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 52.7 kDa. Observed band size: 56 kDa.
Product Details
PA5-144413
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Shigella flexneri IPAD protein (1-332aa)
Conjugate
Form
Liquid
Concentration
3.819 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3091632
Target Information
Required for bacterial invasion of host cells. Controls IpaB and IpaC secretion, and the efficiency with which they are physically inserted into target cell membranes. These proteins are exported via T3SS to form a pore in the host membrane that allows the translocation of the other effectors into the host cytoplasm. Along with IpaB, is essential for both blocking secretion through the Mxi/Spa translocon in the absence of a secretion-inducing signal, and for controlling the level of secretion in the presence of this signal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Invasin IpaDipaD CP0126
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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