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IPO5 Antibody (TA811923) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IPO5 (RC207221, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-IPO5. (1. Positive lysates LY419423 (100 µg) and LC419423 (20 µg) can be purchased separately from OriGene.
Product Details
TA811923
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1C5
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-220 of human IPO5 produced in E.coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Imp5; importin beta-3 subunit; Importin subunit beta-3; Importin-5; IPO 5; karyopherin (importin) beta 3; Karyopherin beta 3; Karyopherin beta-3; MGC2068; RAN binding protein 5; Ran-binding protein 5; Ran_GTP binding protein 5; RanBP 5; RanBP5
Gene Aliases: 1110011C18Rik; 5730478E03Rik; AA409333; C76941; IMB3; imp5; IPO5; KPNB3; Pse1; RANBP5
UniProt ID: (Human) O00410, (Mouse) Q8BKC5
Entrez Gene ID: (Human) 3843, (Mouse) 70572
NLS-bearing protein import into nucleus
ribosomal protein import into nucleus
transport
intracellular protein transport
protein transport
positive regulation of protein import into nucleus
cellular response to amino acid stimulus
protein import into nucleus, docking
protein import into nucleus, translocation
viral process
negative regulation of GTPase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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