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Invitrogen
IQGAP1 Recombinant Rabbit Monoclonal Antibody (JG38-74)
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FIGURE: 1 / 13
IQGAP1 Antibody (MA5-34789) in ICC/IF
Immunofluorescence analysis of IQGAP1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with IQGAP1 Recombinant Rabbit Monoclonal Antibody (JG38-74) (Product # MA5-34789) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, ... View More
Product Details
MA5-34789
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JG38-74
Immunogen
Recombinant protein within Human IQGAP1 aa 10-100
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848697
Product Specific Information
Positive Control: HUVEC, A431, mouse placenta tissue lysate, Hela, SiHa, human lung cancer tissue, human spleen tissue, human placenta tissue, mouse colon tissue.
Target Information
This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cdc42-Rac1 effector protein; DISP2; DISPB; p195; Ras GTPase-activating-like protein IQGAP1; RasGAP-like with IQ motifs
Gene Aliases: AA682088; D7Ertd237e; D7Ertd257e; HUMORFA01; IQGAP1; KIAA0051; mKIAA0051; p195; SAR1
UniProt ID: (Human) P46940, (Mouse) Q9JKF1
Entrez Gene ID: (Human) 8826, (Mouse) 29875
GTPase inhibitor activity
GTPase activator activity
calcium ion binding
protein binding
calmodulin binding
phosphatidylinositol-3,4,5-trisphosphate binding
protein kinase binding
protein phosphatase binding
protein domain specific binding
protein complex binding
protein serine/threonine kinase activator activity
S100 protein binding
Rac GTPase binding
cadherin binding involved in cell-cell adhesion
Rho GTPase binding
regulation of cytokine production
signal transduction
epidermal growth factor receptor signaling pathway
small GTPase mediated signal transduction
fibroblast growth factor receptor signaling pathway
negative regulation of dephosphorylation
cellular response to platelet-derived growth factor stimulus
regulation of GTPase activity
cellular response to fibroblast growth factor stimulus
positive regulation of protein kinase activity
platelet-derived growth factor receptor signaling pathway
cellular response to calcium ion
cellular response to epidermal growth factor stimulus
positive regulation of protein serine/threonine kinase activity
neuron projection extension
negative regulation of GTPase activity
positive regulation of GTPase activity
glomerular visceral epithelial cell development
cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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