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Bethyl Laboratories
IRF1 Polyclonal Antibody
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IRF1 Antibody (A303-376A) in WB
Detection of mouse IRF1 by western blot and immunoprecipitation. Samples: Whole cell lysate from NIH 3T3 cells (M; 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified rabbit anti-IRF1 antibody (Product # A303-376A) used for WB at 0.2 µg/mL (A) and 1 µg/mL (B) and used for IP at 6 µg/mg lysate. IRF1 was also immunoprecipitated by rabbit anti-IRF1 antibodies A303-377A and A303-378A, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 60 seconds (A and B).
Product Details
A303-376A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 200 and 250 of mouse Interferon Regulatory Factor 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppression. Diseases associated with IRF1 include gastric cancer, somatic and lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Interferon regulatory factor 1; IRF-1
Gene Aliases: AU020929; Irf-1; Irf1
UniProt ID: (Mouse) P15314
Entrez Gene ID: (Mouse) 16362
regulatory region DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
immune system process
regulation of adaptive immune response
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
apoptotic process
cell cycle arrest
I-kappaB kinase/NF-kappaB signaling
regulation of gene expression
positive regulation of cell death
positive regulation of type I interferon production
positive regulation of interferon-beta production
positive regulation of natural killer cell differentiation
regulation of MyD88-dependent toll-like receptor signaling pathway
cellular response to interferon-beta
negative regulation of tyrosine phosphorylation of Stat1 protein
CD8-positive, alpha-beta T cell differentiation
positive regulation of interleukin-12 biosynthetic process
innate immune response
regulation of innate immune response
negative regulation of regulatory T cell differentiation
positive regulation of T-helper 1 cell differentiation
negative regulation of T-helper 2 cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
defense response to virus
interferon-gamma-mediated signaling pathway
response to growth hormone
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to peptide hormone stimulus
regulation of CD8-positive, alpha-beta T cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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