Proteintech

IRF5 Polyclonal Antibody

IRF5 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

IRF5 Antibody (10547-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 10547-1-AP (IRF5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

IRF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IRF5 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10547-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 21 publications 21 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 4 publications 4 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IRF5 Fusion Protein Ag0841 (172-498 aa encoded by BC004201)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

10547-1-AP is a rabbit polyclonal antibody raised against the C-terminal of human IRF5.

Immunogen sequence: PTLRPPTLQ PPTLQPPVVL GPPAPDPSPL APPPGNPAGF RELLSEVLEP GPLPASLPPA GEQLLPDLLI SPHMLPLTDL EIKFQYRGRP PRALTISNPH GCRLFYSQLE ATQEQVELFG PISLEQVRFP SPEDIPSDKQ RFYTNQLLDV LDRGLILQLQ GQDLYAIRLC QCKVFWSGPC ASAHDSCPNP IQREVKTKLF SLEHFLNELI LFQKGQTNTP PPFEIFFCFG EEWPDRKPRE KKLITVQVVP VAARLLLEMF SGELSWSADS IRLQISNPDL KDRMVEQFKE LHHIWQSQQR LQPVAQAPPG AGLGVGQGPW PMHPAGMQ (172-498 aa encoded by BC004201)

Target Information

This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neohesperidin Alleviates the Neuropathic Pain Behavior of Rats by Downregulating the P2X4 Receptor.

Neurochemical research

Wang Y,Li C,Xing J,Zhu Y,Sun M,Yin S,Liu J,Zou L,Liang S,Liu S

Wed Mar 01 00:00:00 UTC 2023

Cytoplasmic localization of IRF5 induces Wnt5a/E-cadherin degradation and promotes gastric cancer cells metastasis.

Cancer gene therapy

Du J,Sun C,Liu J,Wang X,Zhao X,Wang Y,Ma Y,Xie H,Qi C,Wang Q,Xia T,Ye F,Zhang Y

Thu Jun 01 00:00:00 UTC 2023

Early macrophage response to obesity encompasses Interferon Regulatory Factor 5 regulated mitochondrial architecture remodelling.

Nature communications

Orliaguet L,Ejlalmanesh T,Humbert A,Ballaire R,Diedisheim M,Julla JB,Chokr D,Cuenco J,Michieletto J,Charbit J,Lindén D,Boucher J,Potier C,Hamimi A,Lemoine S,Blugeon C,Legoix P,Lameiras S,Baudrin LG,Baulande S,Soprani A,Castelli FA,Fenaille F,Riveline JP,Dalmas E,Rieusset J,Gautier JF,Venteclef N,Alzaid F

Tue Aug 30 00:00:00 UTC 2022

Early macrophage response to obesity encompasses Interferon Regulatory Factor 5 regulated mitochondrial architecture remodelling.

Nature communications

Tue Aug 30 00:00:00 UTC 2022

Ccl3 enhances docetaxel chemosensitivity in breast cancer by triggering proinflammatory macrophage polarization.

Journal for immunotherapy of cancer

Sheng D,Ma W,Zhang R,Zhou L,Deng Q,Tu J,Chen W,Zhang F,Gao N,Dong M,Wang D,Li F,Liu Y,He X,Duan S,Zhang L,Liu T,Liu S

Sun May 01 00:00:00 UTC 2022

Acupuncture relieves the visceral pain of diarrhea-predominant irritable bowel syndrome rats by regulating P2X4 expression.

American journal of translational research

Tang HY,Chen XQ,Wang H,Chu HR,Zhu CF,Huang S,Zhang MT,Shen GM

Sat Sep 17 00:00:00 UTC 2022

Distinct roles of PI3Kδ and PI3Kγ in a toluene diisocyanate-induced murine asthma model.

Toxicology

Xu C,Chen S,Deng Y,Song J,Li J,Chen X,Chang P,Yao L,Tang H

Fri Apr 30 00:00:00 UTC 2021

Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl₄/2-AAF.

Pharmaceutical biology

Xu Y,Xu W,Liu W,Chen G,Jiang S,Chen J,Jian X,Zhang H,Liu P,Mu Y

Wed Dec 01 00:00:00 UTC 2021

Neutrophilic Asthma Is Associated With Smoking, High Numbers of IRF5+, and Low Numbers of IL10+ Macrophages.

Frontiers in allergy

Turan N,van der Veen TA,Draijer C,Fattahi F,Ten Hacken NH,Timens W,van Oosterhout AJ,van den Berge M,Melgert BN

Fri Apr 08 00:00:00 UTC 2022

Echinacea Purpurea Extract (cichoric Acid) Exerts an Anti-inflammatory Effect on Yak PBMCs and Regulates the TLR4 Signalling Pathway.

Journal of veterinary research

Xue CH,A SX,Wang MJ,Wu Q,Liu JH,Zhang LF,Wu Y,Wu H,Chai ST

Mon Mar 01 00:00:00 UTC 2021

Silica particles disorganize the polarization of pulmonary macrophages in mice.

Ecotoxicology and environmental safety

Zhao Y,Hao C,Bao L,Wang D,Li Y,Qu Y,Ding M,Zhao A,Yao W

Wed Apr 15 00:00:00 UTC 2020

Rotator cuff repair with biological graft augmentation causes adverse tissue outcomes.

Acta orthopaedica

Rashid MS,Smith RDJ,Nagra N,Wheway K,Watkins B,Snelling S,Dakin SG,Carr AJ

Tue Dec 01 00:00:00 UTC 2020

Prenatal smoke effect on mouse offspring Igf1 promoter methylation from fetal stage to adulthood is organ and sex specific.

American journal of physiology. Lung cellular and molecular physiology

Zeng Z,Meyer KF,Lkhagvadorj K,Kooistra W,Reinders-Luinge M,Xu X,Huo X,Song J,Plösch T,Hylkema MN

Sun Mar 01 00:00:00 UTC 2020

Corilagin Ameliorates Atherosclerosis in Peripheral Artery Disease via the Toll-Like Receptor-4 Signaling Pathway in vitro and in vivo.

Frontiers in immunology

Li Y,Wang Y,Chen Y,Wang Y,Zhang S,Liu P,Chen Z,Song P,Luo L,Luo Y,Dang Y,Zhao L

Tue Apr 20 00:00:00 UTC 2021

Phosphate Groups in the Lipid A Moiety Determine the Effects of LPS on Hepatic Stellate Cells: A Role for LPS-Dephosphorylating Activity in Liver Fibrosis.

Cells

Schippers M,Post E,Eichhorn I,Langeland J,Beljaars L,Malo MS,Hodin RA,Millán JL,Popov Y,Schuppan D,Poelstra K

Thu Dec 17 00:00:00 UTC 2020

Sedum sarmentosum Bunge extract alleviates inflammation and kidney injury via inhibition of M1-macrophage polarization.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Lu H,Cheng S,Wu C,Zheng S,Hong W,Liu L,Bai Y

Sun Sep 01 00:00:00 UTC 2019

Sedum sarmentosum Bunge extract alleviates inflammation and kidney injury via inhibition of M1-macrophage polarization.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Lu H,Cheng S,Wu C,Zheng S,Hong W,Liu L,Bai Y

Sun Sep 01 00:00:00 UTC 2019

Sedum sarmentosum Bunge extract alleviates inflammation and kidney injury via inhibition of M1-macrophage polarization.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Lu H,Cheng S,Wu C,Zheng S,Hong W,Liu L,Bai Y

Sun Sep 01 00:00:00 UTC 2019

Sedum sarmentosum Bunge extract alleviates inflammation and kidney injury via inhibition of M1-macrophage polarization.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Lu H,Cheng S,Wu C,Zheng S,Hong W,Liu L,Bai Y

Sun Sep 01 00:00:00 UTC 2019

Chronic inflammation is a feature of Achilles tendinopathy and rupture.

British journal of sports medicine

Dakin SG,Newton J,Martinez FO,Hedley R,Gwilym S,Jones N,Reid HAB,Wood S,Wells G,Appleton L,Wheway K,Watkins B,Carr AJ

Thu Mar 01 00:00:00 UTC 2018

Chronic inflammation is a feature of Achilles tendinopathy and rupture.

British journal of sports medicine

Dakin SG,Newton J,Martinez FO,Hedley R,Gwilym S,Jones N,Reid HAB,Wood S,Wells G,Appleton L,Wheway K,Watkins B,Carr AJ

Thu Mar 01 00:00:00 UTC 2018

Quercetin ameliorates kidney injury and fibrosis by modulating M1/M2 macrophage polarization.

Biochemical pharmacology

Lu H,Wu L,Liu L,Ruan Q,Zhang X,Hong W,Wu S,Jin G,Bai Y

Wed Aug 01 00:00:00 UTC 2018

Quercetin ameliorates kidney injury and fibrosis by modulating M1/M2 macrophage polarization.

Biochemical pharmacology

Lu H,Wu L,Liu L,Ruan Q,Zhang X,Hong W,Wu S,Jin G,Bai Y

Wed Aug 01 00:00:00 UTC 2018

Quercetin ameliorates kidney injury and fibrosis by modulating M1/M2 macrophage polarization.

Biochemical pharmacology

Lu H,Wu L,Liu L,Ruan Q,Zhang X,Hong W,Wu S,Jin G,Bai Y

Wed Aug 01 00:00:00 UTC 2018

Quercetin ameliorates kidney injury and fibrosis by modulating M1/M2 macrophage polarization.

Biochemical pharmacology

Lu H,Wu L,Liu L,Ruan Q,Zhang X,Hong W,Wu S,Jin G,Bai Y

Wed Aug 01 00:00:00 UTC 2018

Ibuprofen and diclofenac treatments reduce proliferation of pancreatic acinar cells upon inflammatory injury and mitogenic stimulation.

British journal of pharmacology

Bombardo M,Malagola E,Chen R,Rudnicka A,Graf R,Sonda S

Mon Jan 01 00:00:00 UTC 2018

Dual role of YM1+ M2 macrophages in allergic lung inflammation.

Scientific reports

Draijer C,Robbe P,Boorsma CE,Hylkema MN,Melgert BN

Fri Mar 23 00:00:00 UTC 2018

Dual role of YM1+ M2 macrophages in allergic lung inflammation.

Scientific reports

Draijer C,Robbe P,Boorsma CE,Hylkema MN,Melgert BN

Fri Mar 23 00:00:00 UTC 2018

Tumor Suppressor p53 Stimulates the Expression of Epstein-Barr Virus Latent Membrane Protein 1.

Journal of virology

Wang Q,Lingel A,Geiser V,Kwapnoski Z,Zhang L

Sun Oct 15 00:00:00 UTC 2017

Tumor Suppressor p53 Stimulates the Expression of Epstein-Barr Virus Latent Membrane Protein 1.

Journal of virology

Wang Q,Lingel A,Geiser V,Kwapnoski Z,Zhang L

Sun Oct 15 00:00:00 UTC 2017

Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.

Mediators of inflammation

Jiang Z,Song F,Li Y,Xue D,Deng G,Li M,Liu X,Wang Y

Tue Mar 27 00:00:00 UTC 2018

Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.

Mediators of inflammation

Jiang Z,Song F,Li Y,Xue D,Deng G,Li M,Liu X,Wang Y

Tue Mar 27 00:00:00 UTC 2018

Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality.

Scientific reports

Li D,De S,Li D,Song S,Matta B,Barnes BJ

Tue Aug 02 00:00:00 UTC 2016

Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality.

Scientific reports

Li D,De S,Li D,Song S,Matta B,Barnes BJ

Tue Aug 02 00:00:00 UTC 2016

Distinct macrophage phenotypes in allergic and nonallergic lung inflammation.

American journal of physiology. Lung cellular and molecular physiology

Robbe P,Draijer C,Borg TR,Luinge M,Timens W,Wouters IM,Melgert BN,Hylkema MN

Sun Feb 15 00:00:00 UTC 2015

Distinct macrophage phenotypes in allergic and nonallergic lung inflammation.

American journal of physiology. Lung cellular and molecular physiology

Robbe P,Draijer C,Borg TR,Luinge M,Timens W,Wouters IM,Melgert BN,Hylkema MN

Sun Feb 15 00:00:00 UTC 2015

C1q-mediated repression of human monocytes is regulated by leukocyte-associated Ig-like receptor 1 (LAIR-1).

Molecular medicine (Cambridge, Mass.)

Son M,Diamond B

Thu Feb 05 00:00:00 UTC 2015

Characterization of transfusion-elicited acute antibody-mediated rejection in a rat model of kidney transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Huang G,Wilson NA,Reese SR,Jacobson LM,Zhong W,Djamali A

Thu May 01 00:00:00 UTC 2014

Characterization of transfusion-elicited acute antibody-mediated rejection in a rat model of kidney transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Huang G,Wilson NA,Reese SR,Jacobson LM,Zhong W,Djamali A

Thu May 01 00:00:00 UTC 2014

Hepatic Localization of Macrophage Phenotypes during Fibrogenesis and Resolution of Fibrosis in Mice and Humans.

Frontiers in immunology

Beljaars L,Schippers M,Reker-Smit C,Martinez FO,Helming L,Poelstra K,Melgert BN

Wed Sep 24 00:00:00 UTC 2014

Hepatic Localization of Macrophage Phenotypes during Fibrogenesis and Resolution of Fibrosis in Mice and Humans.

Frontiers in immunology

Beljaars L,Schippers M,Reker-Smit C,Martinez FO,Helming L,Poelstra K,Melgert BN

Wed Sep 24 00:00:00 UTC 2014

Hepatic Localization of Macrophage Phenotypes during Fibrogenesis and Resolution of Fibrosis in Mice and Humans.

Frontiers in immunology

Beljaars L,Schippers M,Reker-Smit C,Martinez FO,Helming L,Poelstra K,Melgert BN

Wed Sep 24 00:00:00 UTC 2014

PPE38 of Mycobacterium marinum triggers the cross-talk of multiple pathways involved in the host response, as revealed by subcellular quantitative proteomics.

Journal of proteome research

Wang H,Dong D,Tang S,Chen X,Gao Q

Fri May 03 00:00:00 UTC 2013

PPE38 of Mycobacterium marinum triggers the cross-talk of multiple pathways involved in the host response, as revealed by subcellular quantitative proteomics.

Journal of proteome research

Wang H,Dong D,Tang S,Chen X,Gao Q

Fri May 03 00:00:00 UTC 2013

Characterization of macrophage phenotypes in three murine models of house-dust-mite-induced asthma.

Mediators of inflammation

Draijer C,Robbe P,Boorsma CE,Hylkema MN,Melgert BN

Mon Aug 19 00:00:00 UTC 2013

Characterization of macrophage phenotypes in three murine models of house-dust-mite-induced asthma.

Mediators of inflammation

Draijer C,Robbe P,Boorsma CE,Hylkema MN,Melgert BN

Mon Aug 19 00:00:00 UTC 2013

Interleukin-4-induced β-catenin regulates the conversion of macrophages to multinucleated giant cells.

Molecular immunology

Binder F,Hayakawa M,Choo MK,Sano Y,Park JM

Sat Jun 01 00:00:00 UTC 2013

Interleukin-4-induced β-catenin regulates the conversion of macrophages to multinucleated giant cells.

Molecular immunology

Binder F,Hayakawa M,Choo MK,Sano Y,Park JM

Sat Jun 01 00:00:00 UTC 2013

A study on the immunomodulation of polysaccharopeptide through the TLR4-TIRAP/MAL-MyD88 signaling pathway in PBMCs from breast cancer patients.

Immunopharmacology and immunotoxicology

Wang J,Dong B,Tan Y,Yu S,Bao YX

Thu Aug 01 00:00:00 UTC 2013

A study on the immunomodulation of polysaccharopeptide through the TLR4-TIRAP/MAL-MyD88 signaling pathway in PBMCs from breast cancer patients.

Immunopharmacology and immunotoxicology

Wang J,Dong B,Tan Y,Yu S,Bao YX

Thu Aug 01 00:00:00 UTC 2013

PEGylation of interleukin-10 improves the pharmacokinetic profile and enhances the antifibrotic effectivity in CCl₄-induced fibrogenesis in mice.

Journal of controlled release : official journal of the Controlled Release Society

Mattos A,de Jager-Krikken A,de Haan M,Beljaars L,Poelstra K

Mon Aug 20 00:00:00 UTC 2012

PEGylation of interleukin-10 improves the pharmacokinetic profile and enhances the antifibrotic effectivity in CCl₄-induced fibrogenesis in mice.

Journal of controlled release : official journal of the Controlled Release Society

Mattos A,de Jager-Krikken A,de Haan M,Beljaars L,Poelstra K

Mon Aug 20 00:00:00 UTC 2012

Smoking during pregnancy influences the maternal immune response in mice and humans.

American journal of obstetrics and gynecology

Prins JR,Hylkema MN,Erwich JJ,Huitema S,Dekkema GJ,Dijkstra FE,Faas MM,Melgert BN

Sun Jul 01 00:00:00 UTC 2012

Smoking during pregnancy influences the maternal immune response in mice and humans.

American journal of obstetrics and gynecology

Prins JR,Hylkema MN,Erwich JJ,Huitema S,Dekkema GJ,Dijkstra FE,Faas MM,Melgert BN

Sun Jul 01 00:00:00 UTC 2012

4F decreases IRF5 expression and activation in hearts of tight skin mice.

PloS one

Xu H,Krolikowski JG,Jones DW,Ge ZD,Pagel PS,Pritchard KA,Weihrauch D

Thu Sep 05 00:00:00 UTC 2013

4F decreases IRF5 expression and activation in hearts of tight skin mice.

PloS one

Xu H,Krolikowski JG,Jones DW,Ge ZD,Pagel PS,Pritchard KA,Weihrauch D

Thu Sep 05 00:00:00 UTC 2013

Activation of interferon regulatory factor 5 by site specific phosphorylation.

PloS one

Chang Foreman HC,Van Scoy S,Cheng TF,Reich NC

Thu Aug 02 00:00:00 UTC 2012

Activation of interferon regulatory factor 5 by site specific phosphorylation.

PloS one

Chang Foreman HC,Van Scoy S,Cheng TF,Reich NC

Thu Aug 02 00:00:00 UTC 2012

Activation of interferon regulatory factor 5 by site specific phosphorylation.

PloS one

Chang Foreman HC,Van Scoy S,Cheng TF,Reich NC

Thu Aug 02 00:00:00 UTC 2012

Activation of interferon regulatory factor 5 by site specific phosphorylation.

PloS one

Chang Foreman HC,Van Scoy S,Cheng TF,Reich NC

Thu Aug 02 00:00:00 UTC 2012

Interferon regulatory factor 4 (IRF-4) targets IRF-5 to regulate Epstein-Barr virus transformation.

The Journal of biological chemistry

Xu D,Meyer F,Ehlers E,Blasnitz L,Zhang L

Fri May 20 00:00:00 UTC 2011

Interferon regulatory factor 4 (IRF-4) targets IRF-5 to regulate Epstein-Barr virus transformation.

The Journal of biological chemistry

Xu D,Meyer F,Ehlers E,Blasnitz L,Zhang L

Fri May 20 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: Interferon regulatory factor 5; interferon regulatory factor 5 bone marrow; IRF-5; OTTHUMP00000212907

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Gene Aliases: AW491843; IRF5; mirf5; SLEB10

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UniProt ID: (Human) Q13568, (Mouse) P56477

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Entrez Gene ID: (Human) 3663, (Rat) 296953, (Mouse) 27056

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Function(s)

regulatory region DNA binding transcription factor activity, sequence-specific DNA binding protein binding DNA binding

Process(es)

cytokine-mediated signaling pathway response to peptidoglycan response to muramyl dipeptide positive regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter defense response to virus immune system process transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter innate immune response positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interleukin-12 production interferon-gamma-mediated signaling pathway type I interferon signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IRF5 Polyclonal Antibody

IRF5 Antibody (10547-1-AP) in ICC/IF

Product Details

10547-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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