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IRF5 Antibody (A303-385A) in WB
Detection of mouse IRF5 by western blot and immunoprecipitation. Samples: Whole cell lysate from mouse bone marrow dendritic cells (D; 50 and 15 µg for WB; 1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified rabbit anti-IRF5 antibody (Product # A303-385A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 6 µg/mg lysate. IRF5 was also immunoprecipitated by rabbit anti-IRF5 antibody A303-386A, which recognizes a disparate epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Product Details
A303-385A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 175 and 225 of mouse Interferon Regulatory Factor 5.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Interferon regulatory factor 5; interferon regulatory factor 5 bone marrow; IRF-5; OTTHUMP00000212907
Gene Aliases: AW491843; IRF5; mirf5; SLEB10
UniProt ID: (Human) Q13568, (Mouse) P56477
Entrez Gene ID: (Human) 3663, (Mouse) 27056
immune system process
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cytokine-mediated signaling pathway
response to peptidoglycan
response to muramyl dipeptide
positive regulation of apoptotic process
innate immune response
positive regulation of transcription from RNA polymerase II promoter
defense response to virus
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interleukin-12 production
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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