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FIGURE: 1 / 1
IRF5 Antibody (PA5-88142) in WB
Western blot analysis of IRF5 in extracts of various cell lines. Samples were incubated with IRF5 Polyclonal antibody (Product # PA5-88142) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-88142
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human IRF5 (NP_0010920972)
Conjugate
Form
Liquid
Concentration
0.83 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804685
Product Specific Information
Immunogen sequence: MNQSIPVAPT PPRRVRLKPW LVAQVNSCQY PGLQWVNGEK KLFCIPWRHA TRHGPSQDGD NTIFKAWAKE TGKYTEGVDE ADPAKWKANL RCALNKSRDF RLIYDGPRDM PPQPYKIYEV CSNGPAPTDS QPPEDYSFGA GEEEEEEEEL QRMLPSLSLT EDVKWPPTLQ PPTLRPPTLQ PPTLQPPVVL GPPAPDPSPL; Positive Samples: THP-1, U-937, Rat thymus; Cellular Location: Cytoplasm, Nucleus
Target Information
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Interferon regulatory factor 5; IRF-5; OTTHUMP00000212907
Gene Aliases: IRF5; SLEB10
UniProt ID: (Human) Q13568
Entrez Gene ID: (Human) 3663, (Rat) 296953
cytokine-mediated signaling pathway
response to peptidoglycan
response to muramyl dipeptide
positive regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
defense response to virus
transcription, DNA-templated
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interleukin-12 production
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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