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FIGURE: 1 / 2
IRF5 Antibody (MA5-35069) in WB
Western blot analysis of IRF5 in extracts of various cell lines. Samples were incubated with IRF5 Monoclonal antibody (Product # MA5-35069) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5 min.
Product Details
MA5-35069
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
6L2B5
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 6-151 of human IRF5 (Q13568)
Conjugate
Form
Liquid
Concentration
1.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848974
Product Specific Information
Immunogen sequence: PVAPTPPRRV RLKPWLVAQV NSCQYPGLQW VNGEKKLFCI PWRHATRHGP SQDGDNTIFK AWAKETGKYT EGVDEADPAK WKANLRCALN KSRDFRLIYD GPRDMPPQPY KIYEVCSNGP APTDSQPPED YSFGAGEEEE EEEELQ
Target Information
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Interferon regulatory factor 5; interferon regulatory factor 5 bone marrow; IRF-5; OTTHUMP00000212907
Gene Aliases: AW491843; IRF5; mirf5; SLEB10
UniProt ID: (Human) Q13568, (Mouse) P56477
Entrez Gene ID: (Human) 3663, (Mouse) 27056
immune system process
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cytokine-mediated signaling pathway
response to peptidoglycan
response to muramyl dipeptide
positive regulation of apoptotic process
innate immune response
positive regulation of transcription from RNA polymerase II promoter
defense response to virus
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interleukin-12 production
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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