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Invitrogen
IRF5 Monoclonal Antibody (ALYSCLN), eFluor™ 660, eBioscience™
FIGURE: 1 / 2
IRF5 Antibody (50-9698-42) in Flow
Normal human peripheral blood cells were stained with Anti-Human CD3 PerCP-eFluor® 710 (Product # 46-0036-42), Anti-Human CD11b FITC (Product # 11-0118-42), Anti-Human CD123 PE (Product # 12-1239-42), and Fixable Viability Dye eFluor® 780 (Product # 65-0865-14). Intracellular staining with Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (left) or Anti-Human IRF5 eFluor® 660 (right) was performed using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Viable single cells in the CD3+ (ora... View More
Product Details
50-9698-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
ALYSCLN
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574347
Product Specific Information
Description: The ALYSCLN monoclonal antibody reacts with human IRF5, a 60-63 kDa member of the interferon regulatory factor family of transcription factors that have diverse roles in host defense against viruses. IRF5 was originally identified as an important regulator of IFN alpha and IFN beta and more recently has been shown to be regulated by these interferons. IRF5 is also involved in induction of pro-inflammatory cytokines including IL-6, IL-12 and TNF alpha in response to TLR signaling. Specifically, it has been shown that MyD88 and TRAF6 interact directly with IRF5 in response to TLR activation and this interaction results in IRF5 nuclear translocation. Additionally, IRF5 has been shown to have both p53-dependent and independent effects in regulation of cell cycle and apoptosis. Genetic polymorphisms in the IRF5 locus have been implicated in many autoimmune diseases including Sjogren's syndrome, inflammatory bowel disease, multiple sclerosis and systemic lupus erythematosus.
Recently, a role for IRF5 in the polarization of classically activated (M1) macrophages has been identified. In these macrophages, IRF5 directly upregulates expression of IL-12 and IL-23 and represses IL-10. Moreover, exogenous expression of IRF5 in M2-polarized (alternatively activated) macrophages induces expression of M1-related cytokines and chemokines. IRF5 is expressed at highest levels in monocytes, macrophages and plasmacytoid dendritic cells, but is also expressed in B cells and T cells.
Applications Reported: This ALYSCLN antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This ALYSCLN antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Interferon regulatory factor 5; IRF-5; OTTHUMP00000212907
Gene Aliases: IRF5; SLEB10
UniProt ID: (Human) Q13568
Entrez Gene ID: (Human) 3663
transcription, DNA-templated
response to peptidoglycan
response to muramyl dipeptide
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interleukin-12 production
positive regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
defense response to virus
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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