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Invitrogen
IRF7 Polyclonal Antibody
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FIGURE: 1 / 4
IRF7 Antibody (PA5-85724) in ICC/IF
Immunofluorescence analysis of Interferon regulatory factor 7 was performed using 70% confluent log phase HeLa IFN alpha, 500U/mL for 12 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with IRF7 Polyclonal Antibody (Product # PA5-85724) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibod... View More
Product Details
PA5-85724
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human IRF7. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.32 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2792863
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Rhesus Monkey (91%).
Positive Control: Jurkat, Raji, NCI-H929.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-a/b genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Interferon regulatory factor 7; interferon regulatory factor-7H; IRF-7
Gene Aliases: IMD39; IRF-7H; IRF7; IRF7A; IRF7B; IRF7C; IRF7H
UniProt ID: (Human) Q92985
Entrez Gene ID: (Human) 3665
negative regulation of transcription from RNA polymerase II promoter
regulation of adaptive immune response
transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
response to virus
immunoglobulin mediated immune response
establishment of viral latency
regulation of type I interferon production
positive regulation of type I interferon production
interferon-alpha production
interferon-beta production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
regulation of MyD88-dependent toll-like receptor signaling pathway
regulation of MyD88-independent toll-like receptor signaling pathway
TRIF-dependent toll-like receptor signaling pathway
MDA-5 signaling pathway
innate immune response
type I interferon biosynthetic process
regulation of monocyte differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of immune response
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
positive regulation of type I interferon-mediated signaling pathway
negative regulation of macrophage apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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