Invitrogen

IRF7 Monoclonal Antibody (MNGPKL), PE, eBioscience™

3 Published Figures

3 References

View all (19) IRF7 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (3)

FIGURE: 1 / 4

IRF7 Antibody (12-5829-82) in Flow

Mouse splenocytes were untreated (right, orange histogram) or treated with CpG ODN 2395 (right, purple histogram) for 5 hours. Using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, the cells were then intracellularly stained with Anti-Mouse CD317 (BST2, PDCA-1) FITC (Product # 11-3172-82), Anti-Human/Mouse CD45R (B220) eFluor® 450 (Product # 48-0452-82), and 0.125 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (right, blue histogram) or 0.125 µg of Anti-Mouse IRF7 PE. Viable CD317+... View More

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Product Details

12-5829-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MNGPKL

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2572629

Product Specific Information

Description: The monoclonal antibody MNGPKL reacts with mouse interferon regulatory factor 7 (IRF7), a 54 kDa transcription factor essential for induction of the antiviral innate immune response and IFN alpha production. B cells, plasmacytoid dendritic cells (pDC), and monocytes constitutively express IRF7 in the cytoplasm until activation by viral infection, double-stranded RNA or Toll-like receptor (TLR) signaling. CpG ODNs and single-stranded RNA, recognized by CD289 (TLR9) and TLR7, respectively, trigger IRF7 activation and upregulation. This activation promotes type I IFN response to disrupt pathogen and viral infection. These events causeIRF7 phosphorylation and translocation to the nucleus where it forms a homodimer to affect transcription.

Applications Reported: This MNGPKL antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MNGPKL antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-a/b genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Characterization of proteogenomic signatures of differentiation of CD4+ T cell subsets.

DNA research : an international journal for rapid publication of reports on genes and genomes

Kanno T,Konno R,Miyako K,Nakajima T,Yokoyama S,Sasamoto S,Asou HK,Ohzeki J,Kawashima Y,Hasegawa Y,Ohara O,Endo Y

Published figure using IRF7 monoclonal antibody (Product # 12-5829-82) in Flow Cytometry

Wed Feb 01 00:00:00 UTC 2023

SCD2-mediated cooperative activation of IRF3-IRF9 regulatory circuit controls type I interferon transcriptome in CD4⁺ T cells.

Frontiers in immunology

Kanno T,Miyako K,Nakajima T,Yokoyama S,Sasamoto S,Asou HK,Ohara O,Nakayama T,Endo Y

Published figure using IRF7 monoclonal antibody (Product # 12-5829-82) in Flow Cytometry

Thu Sep 08 00:00:00 UTC 2022

Single-Cell Profiling Defines Transcriptomic Signatures Specific to Tumor-Reactive versus Virus-Responsive CD4⁺ T Cells.

Cell reports

Magen A,Nie J,Ciucci T,Tamoutounour S,Zhao Y,Mehta M,Tran B,McGavern DB,Hannenhalli S,Bosselut R

Published figure using IRF7 monoclonal antibody (Product # 12-5829-82) in Flow Cytometry

Tue Dec 03 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: Interferon regulatory factor 7; IRF-7

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Gene Aliases: Irf7

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UniProt ID: (Mouse) P70434

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Entrez Gene ID: (Mouse) 54123

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Function(s)

regulatory region DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding

Process(es)

immune system process regulation of adaptive immune response transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter immunoglobulin mediated immune response positive regulation of type I interferon production interferon-alpha production interferon-beta production positive regulation of interferon-alpha production positive regulation of interferon-beta production regulation of MyD88-dependent toll-like receptor signaling pathway regulation of MyD88-independent toll-like receptor signaling pathway innate immune response regulation of innate immune response type I interferon biosynthetic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of type I interferon-mediated signaling pathway

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IRF7 Monoclonal Antibody (MNGPKL), PE, eBioscience™

IRF7 Antibody (12-5829-82) in Flow

Product Details

12-5829-82

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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