Invitrogen

IRF8 Monoclonal Antibody (V3GYWCH), APC, eBioscience™

7 Published Figures

14 References

View all (27) IRF8 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (7)

FIGURE: 1 / 8

IRF8 Antibody (17-9852-82) in Flow

Surface staining of normal human peripheral blood cells with Anti-Human CD123 FITC (Product # 11-1239-42) followed by intracellular staining with 0.125 µg of Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or 0.125 µg of Anti-Human/Mouse IRF8 APC (right) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the monocyte gate were used for analysis.

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Product Details

17-9852-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

V3GYWCH

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2573318

Product Specific Information

Description: The V3GYWCH monoclonal antibody reacts with human and mouse interferon regulatory factor 8 (IRF8; ICSBP). IRF8 is a 50 kDa transcription factor that plays a critical role in the development of dendritic cells (DC). Specifically, IRF8 is essential in the development and differentiation of plasmacytoid DC (pDC) and CD8a+ DC. IRF8-/- mice are deficient in both pDC and CD8a+ DC populations. CD8a- DC are present in normal numbers in IRF8-/- mice but fail to mature upon Toll-like receptor signaling and are functionally impaired in response to in vivo microbial stimulation. IRF8 deficiency does not affect the frequency or viability of DC precursors and as a result retroviral IRF8 transduction restores pDC development from DC progenitors in IRF8-/- mice.

Applications Reported: This V3GYWCH antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This V3GYWCH antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to BestProtocols®: Protocol B: One-step protocol: intracellular (nuclear) proteins located under the Resources Tab online. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor. It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes. IRF8 plays a negative regulatory role in cells of the immune system. It is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells. This leads to recognition of AICE sequence, an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of the genes. Mutations in the gene can result in immunodeficiencey 32A and 32B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Assessing in vivo presentation of exogenous antigen in the tumor microenvironment in mice.

STAR protocols

Nixon BG,Ji L,Li MO

17-9852-82 was used in Flow cytometry/Cell sorting to describe a stringent bone marrow chimera system to demonstrate presentation of exogenous antigen that is acquired and processed in the tumor microenvironment.

Wed Mar 22 00:00:00 UTC 2023

Germinal center expansion but not plasmablast differentiation is proportional to peptide-MHCII density via CD40-CD40L signaling strength.

Cell reports

Jing Z,McCarron MJ,Dustin ML,Fooksman DR

17-9852-82 was used in Flow Cytometry to establish a caged OVA peptide, which is readily detected by OVA-specific TFH cells after photo-uncaging.

Tue May 03 00:00:00 UTC 2022

DNA methylation signatures of Alzheimer's disease neuropathology in the cortex are primarily driven by variation in non-neuronal cell-types.

Nature communications

Shireby G,Dempster EL,Policicchio S,Smith RG,Pishva E,Chioza B,Davies JP,Burrage J,Lunnon K,Seiler Vellame D,Love S,Thomas A,Brookes K,Morgan K,Francis P,Hannon E,Mill J

17-9852-82 was used in Immunocytochemistry-immunofluorescence to highlight the power of utilizing multiple measures of neuropathology to identify epigenetic signatures of AD and the importance of characterizing disease-associated variation in purified cell-types.

Sat Sep 24 00:00:00 UTC 2022

The transcription factor Bach2 negatively regulates murine natural killer cell maturation and function.

eLife

Li S,Bern MD,Miao B,Fan C,Xing X,Inoue T,Piersma SJ,Wang T,Colonna M,Kurosaki T,Yokoyama WM

17-9852-82 was used in Flow cytometry/Cell sorting to investigate the role of Bach2 in natural killer (NK) cell development, revealing that Bach2 expression peaked in immature CD27+CD11b+ cells, and its deficiency led to increased proportions of terminally differentiated NK cells with enhanced granzyme and cytokine production, as well as improved NK cell-mediated control of tumor metastasis.

Mon Oct 03 00:00:00 UTC 2022

Inflammatory Type 2 cDCs Acquire Features of cDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection.

Immunity

Bosteels C,Neyt K,Vanheerswynghels M,van Helden MJ,Sichien D,Debeuf N,De Prijck S,Bosteels V,Vandamme N,Martens L,Saeys Y,Louagie E,Lesage M,Williams DL,Tang SC,Mayer JU,Ronchese F,Scott CL,Hammad H,Guilliams M,Lambrecht BN

Published figure using IRF8 monoclonal antibody (Product # 17-9852-82) in Flow Cytometry

Tue Jun 16 00:00:00 UTC 2020

Single-Cell RNA-Seq Mapping of Human Thymopoiesis Reveals Lineage Specification Trajectories and a Commitment Spectrum in T Cell Development.

Immunity

Le J,Park JE,Ha VL,Luong A,Branciamore S,Rodin AS,Gogoshin G,Li F,Loh YE,Camacho V,Patel SB,Welner RS,Parekh C

17-9852-82 was used in Flow Cytometry to isolate CD34+ and CD34- progenitor cells in the human postnatal thymus using single-cell RNA sequencing.

Tue Jun 16 00:00:00 UTC 2020

CD103⁺ cDC1 and endogenous CD8⁺ T cells are necessary for improved CD40L-overexpressing CAR T cell antitumor function.

Nature communications

Kuhn NF,Lopez AV,Li X,Cai W,Daniyan AF,Brentjens RJ

Published figure using IRF8 monoclonal antibody (Product # 17-9852-82) in Flow Cytometry

Wed Dec 02 00:00:00 UTC 2020

Androgen Receptor Signaling Positively Regulates Monocytic Development.

Frontiers in immunology

Consiglio CR,Gollnick SO

17-9852 was used in Flow cytometry/Cell sorting to investigate the role of androgen signaling in monocytic differentiation with a genetic mouse model of myeloid androgen receptor (AR) knockout (MARKO) and pharmacological inhibition of AR.

Thu Apr 29 00:00:00 UTC 2021

Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity.

Cell reports

Leylek R,Alcántara-Hernández M,Granja JM,Chavez M,Perez K,Diaz OR,Li R,Satpathy AT,Chang HY,Idoyaga J

17-9852 was used in Flow cytometry/Cell sorting to investigate analyze global chromatin accessibility profiles across resting and stimulated human DC subsets by means of the assay for transposase-accessible chromatin using sequencing (ATAC-seq).

Tue Sep 22 00:00:00 UTC 2020

Lower Interferon Regulatory Factor-8 Expression in Peripheral Myeloid Cells Tracks With Adverse Central Nervous System Outcomes in Treated HIV Infection.

Frontiers in immunology

D'Antoni ML,Kallianpur KJ,Premeaux TA,Corley MJ,Fujita T,Laws EI,Ogata-Arakaki D,Chow DC,Khadka VS,Shikuma CM,Ndhlovu LC

Published figure using IRF8 monoclonal antibody (Product # 17-9852-82) in Flow Cytometry

Tue Nov 03 00:00:00 UTC 2020

NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control.

Cell

Böttcher JP,Bonavita E,Chakravarty P,Blees H,Cabeza-Cabrerizo M,Sammicheli S,Rogers NC,Sahai E,Zelenay S,Reis e Sousa C

17-9852 was used in Flow cytometry/Cell sorting to reveal a cellular and molecular checkpoint for intratumoral cDC1 recruitment that is targeted by tumour-derived PGE2for immune evasion.

Thu Feb 22 00:00:00 UTC 2018

Batf3 selectively determines acquisition of CD8⁺ dendritic cell phenotype and function.

Immunology and cell biology

Chandra J,Kuo PT,Hahn AM,Belz GT,Frazer IH

Wed Feb 01 00:00:00 UTC 2017

Beta-catenin signaling drives differentiation and proinflammatory function of IRF8-dependent dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Cohen SB,Smith NL,McDougal C,Pepper M,Shah S,Yap GS,Acha-Orbea H,Jiang A,Clausen BE,Rudd BD,Denkers EY

Thu Jan 01 00:00:00 UTC 2015

Cell-intrinsic expression of TLR9 in autoreactive B cells constrains BCR/TLR7-dependent responses.

Journal of immunology (Baltimore, Md. : 1950)

Nündel K,Green NM,Shaffer AL,Moody KL,Busto P,Eilat D,Miyake K,Oropallo MA,Cancro MP,Marshak-Rothstein A

Sun Mar 15 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: H-ICSBP; ICSBP; interferon consensus sequence binding protein 1; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; IRF-8

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Gene Aliases: AI893568; H-ICSBP; ICSBP; ICSBP1; IMD32A; IMD32B; IRF-8; IRF8; Myls

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UniProt ID: (Human) Q02556, (Mouse) P23611

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Entrez Gene ID: (Human) 3394, (Mouse) 15900

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Function(s)

regulatory region DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated phagocytosis immune response myeloid cell differentiation positive regulation of interferon-gamma production positive regulation of interleukin-12 production defense response to bacterium defense response to protozoan negative regulation of growth of symbiont in host positive regulation of transcription initiation from RNA polymerase II promoter interferon-gamma-mediated signaling pathway type I interferon signaling pathway cellular response to lipopolysaccharide

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IRF8 Monoclonal Antibody (V3GYWCH), APC, eBioscience™

IRF8 Antibody (17-9852-82) in Flow

Product Details

17-9852-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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