Invitrogen

IRF8 Monoclonal Antibody (V3GYWCH), PerCP-eFluor™ 710, eBioscience™

4 Published Figures

9 References

View all (27) IRF8 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

FIGURE: 1 / 5

IRF8 Antibody (46-9852-82) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD123 PE (Product # 12-1239-42) followed by fixation and permeablization using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells were then stained intracellularly with 0.25 µg of Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (left) or 0.25 µg of Anti-Human/Mouse IRF8 PerCP-eFluor® 710 (right). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.

Product Image Gallery

Product Details

46-9852-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

V3GYWCH

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2573904

Product Specific Information

Description: The V3GYWCH monoclonal antibody reacts with human and mouse interferon regulatory factor 8 (IRF8; ICSBP). IRF8 is a 50 kDa transcription factor that plays a critical role in the development of dendritic cells (DC). Specifically, IRF8 is essential in the development and differentiation of plasmacytoid DC (pDC) and CD8a+ DC. IRF8-/- mice are deficient in both pDC and CD8a+ DC populations. CD8a- DC are present in normal numbers in IRF8-/- mice but fail to mature upon Toll-like receptor signaling and are functionally impaired in response to in vivo microbial stimulation. IRF8 deficiency does not affect the frequency or viability of DC precursors and as a result retroviral IRF8 transduction restores pDC development from DC progenitors in IRF8-/- mice.

Applications Reported: This V3GYWCH antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This V3GYWCH antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to BestProtocols®: Protocol B: One-step protocol: intracellular (nuclear) proteins located under the Resources Tab online. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor. It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes. IRF8 plays a negative regulatory role in cells of the immune system. It is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells. This leads to recognition of AICE sequence, an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of the genes. Mutations in the gene can result in immunodeficiencey 32A and 32B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Toll-like receptor mediated inflammation directs B cells towards protective antiviral extrafollicular responses.

Nature communications

Lam JH,Baumgarth N

46-9852-82 was used in Flow cytometry/Cell sorting to show that influenza infection rapidly induces EFRs, generating protective antibodies via Toll-like receptor (TLR)-mediated mechanisms that are both B cell intrinsic and extrinsic.

Wed Jul 05 00:00:00 UTC 2023

Inflammatory Type 2 cDCs Acquire Features of cDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection.

Immunity

Bosteels C,Neyt K,Vanheerswynghels M,van Helden MJ,Sichien D,Debeuf N,De Prijck S,Bosteels V,Vandamme N,Martens L,Saeys Y,Louagie E,Lesage M,Williams DL,Tang SC,Mayer JU,Ronchese F,Scott CL,Hammad H,Guilliams M,Lambrecht BN

46-9852 was used in Flow cytometry/Cell sorting to show that monocyte-derived inflammatory DCs’’ are a mixture of monocyte-derived MCs that have little migratory and APC potential and bona fide pre-cDCderived, CD26-expressing inf-cDC2s that depend on Flt3L but not on GM-CSF.

Tue Jun 16 00:00:00 UTC 2020

CD103⁺ cDC1 and endogenous CD8⁺ T cells are necessary for improved CD40L-overexpressing CAR T cell antitumor function.

Nature communications

Kuhn NF,Lopez AV,Li X,Cai W,Daniyan AF,Brentjens RJ

46-9852 was used in Flow cytometry/Cell sorting to demonstrate the stimulatory effect of CD40L-overexpressing CAR T cells on innate and adaptive immune cells, and provide a rationale for using CD40L-overexpressing CAR T cells to improve immunotherapy responses.

Wed Dec 02 00:00:00 UTC 2020

CD103⁺ cDC1 and endogenous CD8⁺ T cells are necessary for improved CD40L-overexpressing CAR T cell antitumor function.

Nature communications

Kuhn NF,Lopez AV,Li X,Cai W,Daniyan AF,Brentjens RJ

Wed Dec 02 00:00:00 UTC 2020

Protein Tyrosine Phosphatase Non-Receptor Type 2 Function in Dendritic Cells Is Crucial to Maintain Tissue Tolerance.

Frontiers in immunology

Hering L,Katkeviciute E,Schwarzfischer M,Busenhart P,Gottier C,Mrdjen D,Komuczki J,Wawrzyniak M,Lang S,Atrott K,Becher B,Rogler G,Scharl M,Spalinger MR

46-9852 was used in Flow cytometry/Cell sorting to show dendritic cell PTPN2 might explain the central role for PTPN2 in the immune system to maintain immune tolerance.

Mon Apr 12 00:00:00 UTC 2021

Biallelic mutations in IRF8 impair human NK cell maturation and function.

The Journal of clinical investigation

Mace EM,Bigley V,Gunesch JT,Chinn IK,Angelo LS,Care MA,Maisuria S,Keller MD,Togi S,Watkin LB,LaRosa DF,Jhangiani SN,Muzny DM,Stray-Pedersen A,Coban Akdemir Z,Smith JB,Hernández-Sanabria M,Le DT,Hogg GD,Cao TN,Freud AG,Szymanski EP,Savic S,Collin M,Cant AJ,Gibbs RA,Holland SM,Caligiuri MA,Ozato K,Paust S,Doody GM,Lupski JR,Orange JS

46-9852 was used in Flow cytometry/Cell sorting to indicate that human NK cells require IRF8 for development and that dysregulation of this results in severe human disease.

Tue Jan 03 00:00:00 UTC 2017

Batf3 selectively determines acquisition of CD8⁺ dendritic cell phenotype and function.

Immunology and cell biology

Chandra J,Kuo PT,Hahn AM,Belz GT,Frazer IH

Published figure using IRF8 monoclonal antibody (Product # 46-9852-82) in Flow Cytometry

Wed Feb 01 00:00:00 UTC 2017

Batf3 maintains autoactivation of Irf8 for commitment of a CD8α(+) conventional DC clonogenic progenitor.

Nature immunology

Grajales-Reyes GE,Iwata A,Albring J,Wu X,Tussiwand R,Kc W,Kretzer NM,Briseño CG,Durai V,Bagadia P,Haldar M,Schönheit J,Rosenbauer F,Murphy TL,Murphy KM

46-9852 was used in Flow cytometry/Cell sorting to investigate the role of the transcription factors Batf3 and IRF8 in the development of CD8α(+) conventional dendritic cells (cDCs)..

Wed Jul 01 00:00:00 UTC 2015

Beta-catenin signaling drives differentiation and proinflammatory function of IRF8-dependent dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Cohen SB,Smith NL,McDougal C,Pepper M,Shah S,Yap GS,Acha-Orbea H,Jiang A,Clausen BE,Rudd BD,Denkers EY

Thu Jan 01 00:00:00 UTC 2015

Cell-intrinsic expression of TLR9 in autoreactive B cells constrains BCR/TLR7-dependent responses.

Journal of immunology (Baltimore, Md. : 1950)

Nündel K,Green NM,Shaffer AL,Moody KL,Busto P,Eilat D,Miyake K,Oropallo MA,Cancro MP,Marshak-Rothstein A

Sun Mar 15 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: H-ICSBP; ICSBP; interferon consensus sequence binding protein 1; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; IRF-8

View more View less

Gene Aliases: AI893568; H-ICSBP; ICSBP; ICSBP1; IMD32A; IMD32B; IRF-8; IRF8; Myls

View more View less

UniProt ID: (Human) Q02556, (Mouse) P23611

View more View less

Entrez Gene ID: (Human) 3394, (Mouse) 15900

View more View less

Function(s)

regulatory region DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated phagocytosis immune response myeloid cell differentiation positive regulation of interferon-gamma production positive regulation of interleukin-12 production defense response to bacterium defense response to protozoan negative regulation of growth of symbiont in host positive regulation of transcription initiation from RNA polymerase II promoter interferon-gamma-mediated signaling pathway type I interferon signaling pathway cellular response to lipopolysaccharide

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IRF8 Monoclonal Antibody (V3GYWCH), PerCP-eFluor™ 710, eBioscience™

IRF8 Antibody (46-9852-82) in Flow

Product Details

46-9852-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!