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Invitrogen
IRG1 Polyclonal Antibody
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FIGURE: 1 / 4
IRG1 Antibody (PA5-102893) in ICC/IF
Immunofluorescent analysis of IRG1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and IRG1 polyclonal antibody (Product # PA5-102893) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-102893
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ACOD1(Accession A6NK06), corresponding to amino acid residues I439-S481.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815944
Product Specific Information
Antibody detects endogenous levels of total IRG1.
Target Information
Involved in the inhibition of the inflammatory response. Acts as a negative regulator of the Toll-like receptors (TLRs)- mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages. Involved in antimicrobial response of innate immune cells; IRG1- mediated itaconic acid production contributes to the antimicrobial activity of macrophages. Plays a role in the embryo implantation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Aconitate decarboxylase; Aconitate decarboxylase 1; CAD; Cis-aconitate decarboxylase; Cis-aconitic acid decarboxylase; Immune-responsive gene 1 protein; immune-responsive gene 1 protein homolog; immunoresponsive 1 homolog
Gene Aliases: ACOD1; CAD; IRG1
UniProt ID: (Human) A6NK06
Entrez Gene ID: (Human) 730249
positive regulation of antimicrobial humoral response
defense response
inflammatory response
embryo implantation
metabolic process
negative regulation of NF-kappaB transcription factor activity
negative regulation of type I interferon production
negative regulation of toll-like receptor 2 signaling pathway
negative regulation of toll-like receptor 4 signaling pathway
cellular response to interferon-beta
negative regulation of innate immune response
negative regulation of inflammatory response
cellular response to molecule of bacterial origin
cellular response to lipopolysaccharide
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to progesterone stimulus
tolerance induction to lipopolysaccharide
positive regulation of reactive oxygen species metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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