Proteintech

IRS1 Polyclonal Antibody

IRS1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

IRS1 Antibody (17509-1-AP) in ICC/IF

Immunofluorescent analysis of A549 cells using 17509-1-AP (IRS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

IRS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IRS1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

17509-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 44 publications 44 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Dot blot (DB)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Fish, Human, Mouse, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IRS1 Fusion Protein Ag11714 (895-1243 aa encoded by BC053895)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EYVNIEFGS DQSGYLSGPV AFHSSPSVRC PSQLQPAPRE EETGTEEYMK MDLGPGRRAA WQESTGVEMG RLGPAPPGAA SICRPTRAVP SSRGDYMTMQ MSCPRQSYVD TSPAAPVSYA DMRTGIAAEE VSLPRATMAA ASSSSAASAS PTGPQGAAEL AAHSSLLGGP QGPGGMSAFT RVNLSPNRNQ SAKVIRADPQ GCRRRHSSET FSSTPSATRV GNTVPFGAGA AVGGGGGSSS SSEDVKRHSS ASFENVWLRP GELGGAPKEP AKLCGAAGGL ENGLNYIDLD LVKDFKQCPQ ECTPEPQPPP PPPPHQPLGS GESSSTRRSS EDLSAYASIS FQKQPEDRQ (895-1243 aa encoded by BC053895)

Target Information

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Thu Jan 26 00:00:00 UTC 2023

Overexpression of Insulin Receptor Substrate 1 (IRS1) Relates to Poor Prognosis and Promotes Proliferation, Stemness, Migration, and Oxidative Stress Resistance in Cholangiocarcinoma.

International journal of molecular sciences

Kaewlert W,Sakonsinsiri C,Lert-Itthiporn W,Ungarreevittaya P,Pairojkul C,Pinlaor S,Murata M,Thanan R

Thu Jan 26 00:00:00 UTC 2023

Visceral adipose tissue-directed human kallistatin gene therapy improves adipose tissue remodeling and metabolic health in obese mice.

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Jatrorrhizine from Rhizoma Coptidis exerts an anti-obesity effect in db/db mice.

Journal of ethnopharmacology

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Tue Feb 01 00:00:00 UTC 2022

Upregulation of Klotho Aggravates Insulin Resistance in Gestational Diabetes Mellitus Trophoblast Cells.

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Lin L,Wang X,Zhao W,Chen Y

Mon Nov 07 00:00:00 UTC 2022

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Tue Nov 01 00:00:00 UTC 2022

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Zhou K,Chen Q,Chen J,Liang D,Feng W,Liu M,Wang Q,Wang R,Ouyang Q,Quan C,Chen S

Tue Jul 05 00:00:00 UTC 2022

Spatiotemporal regulation of insulin signaling by liquid-liquid phase separation.

Cell discovery

Zhou K,Chen Q,Chen J,Liang D,Feng W,Liu M,Wang Q,Wang R,Ouyang Q,Quan C,Chen S

Tue Jul 05 00:00:00 UTC 2022

Loss of thymidine phosphorylase activity disrupts adipocyte differentiation and induces insulin-resistant lipoatrophic diabetes.

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Li H,Dun Y,Zhang W,You B,Liu Y,Fu S,Qiu L,Cheng J,Ripley-Gonzalez JW,Liu S

Sat May 15 00:00:00 UTC 2021

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Ginsenoside Rg5 Improves Insulin Resistance and Mitochondrial Biogenesis of Liver via Regulation of the Sirt1/PGC-1α Signaling Pathway in db/db Mice.

Journal of agricultural and food chemistry

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Tue Sep 28 00:00:00 UTC 2021

DPHC From Alpinia officinarum Ameliorates Oxidative Stress and Insulin Resistance via Activation of Nrf2/ARE Pathway in db/db Mice and High Glucose-Treated HepG2 Cells.

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Fri Feb 04 00:00:00 UTC 2022

miR-203 inhibits cell proliferation and ERK pathway in prostate cancer by targeting IRS-1.

BMC cancer

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Thu Nov 12 00:00:00 UTC 2020

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Fish physiology and biochemistry

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Mon Apr 01 00:00:00 UTC 2019

Dietary leucine affects glucose metabolism and lipogenesis involved in TOR/PI3K/Akt signaling pathway for juvenile blunt snout bream Megalobrama amblycephala.

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Therapeutic potential of NaoXinTong Capsule on the developed diabetic nephropathy in db/db mice.

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Hydroxychloroquine Improves Obesity-Associated Insulin Resistance and Hepatic Steatosis by Regulating Lipid Metabolism.

Frontiers in pharmacology

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Toxicology

Wang F,Chang C,Li R,Zhang Z,Jiang H,Zeng N,Li D,Chen L,Xiao Y,Chen W,Wang Q

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Inhibition of insulin resistance by PGE1 via autophagy-dependent FGF21 pathway in diabetic nephropathy.

Scientific reports

Wei W,An XR,Jin SJ,Li XX,Xu M

Mon Jan 08 00:00:00 UTC 2018

Inhibition of insulin resistance by PGE1 via autophagy-dependent FGF21 pathway in diabetic nephropathy.

Scientific reports

Wei W,An XR,Jin SJ,Li XX,Xu M

Mon Jan 08 00:00:00 UTC 2018

NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice.

Scientific reports