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Invitrogen
IRS1 Monoclonal Antibody (10I3)
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FIGURE: 1 / 8
IRS1 Antibody (MA5-36222) in ICC/IF
Immunocytochemistry analysis of IRS1 in MCF7 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in IRS1 monoclonal antibody (Product # MA5-36222) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.
Product Details
MA5-36222
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
10I3
Immunogen
E.coli-derived human IRS1 recombinant protein (Position: S1041-Q1242).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2884056
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human A549 whole cell, human T-47D whole cell, human Caco-2 whole cell, human SW620 whole cell, human Hela whole cell, human Raji whole cell. IHC: human colon cancer tissue, human lung cancer tissue, human placenta tissue. ICC/IF: MCF7 cell. Flow: PC-3 cell, U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Insulin receptor substrate 1; IRS-1
Gene Aliases: HIRS-1; IRS1
UniProt ID: (Human) P35568
Entrez Gene ID: (Human) 3667
signal transducer activity
transmembrane receptor protein tyrosine kinase adaptor activity
protein kinase C binding
Ras guanyl-nucleotide exchange factor activity
insulin receptor binding
insulin-like growth factor receptor binding
protein binding
1-phosphatidylinositol-3-kinase activity
SH2 domain binding
phosphatidylinositol 3-kinase binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
MAPK cascade
signal transduction
positive regulation of cell proliferation
insulin receptor signaling pathway
positive regulation of glucose metabolic process
phosphatidylinositol 3-kinase signaling
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of fatty acid beta-oxidation
response to insulin
cellular response to insulin stimulus
phosphatidylinositol-3-phosphate biosynthetic process
glucose homeostasis
response to peptide hormone
positive regulation of GTPase activity
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of glycogen biosynthetic process
positive regulation of glucose import
negative regulation of insulin receptor signaling pathway
positive regulation of insulin receptor signaling pathway
negative regulation of insulin secretion
phosphatidylinositol phosphorylation
insulin-like growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
positive regulation of glucose import in response to insulin stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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