Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 4
IRS2 Antibody (PA5-14897) in ICC/IF
Immunocytochemistry analysis of IRS2 in HeLa cells. Samples were incubated with IRS2 polyclonal antibody (Product # PA5-14897) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). IRS2 immunoreactivity is localized to Cytoplasm signif... View More
Product Details
PA5-14897
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 1309-1338 amino acids from the C-terminal region of human IRS2
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Size-exclusion - Dialysis, Ammonium sulfate precipitation
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2125800
Target Information
This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Insulin receptor substrate 2; IRS-2
Gene Aliases: IRS-2; IRS2
UniProt ID: (Human) Q9Y4H2
Entrez Gene ID: (Human) 8660
signal transducer activity
Ras guanyl-nucleotide exchange factor activity
insulin receptor binding
protein binding
1-phosphatidylinositol-3-kinase activity
protein kinase binding
protein phosphatase binding
protein domain specific binding
phosphatidylinositol 3-kinase binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
14-3-3 protein binding
MAPK cascade
positive regulation of mesenchymal cell proliferation
negative regulation of B cell apoptotic process
glucose metabolic process
signal transduction
brain development
cell proliferation
positive regulation of cell proliferation
insulin receptor signaling pathway
response to glucose
negative regulation of plasma membrane long-chain fatty acid transport
positive regulation of glucose metabolic process
regulation of phosphatidylinositol 3-kinase signaling
regulation of lipid metabolic process
positive regulation of cell migration
mammary gland development
positive regulation of B cell proliferation
positive regulation of fatty acid beta-oxidation
positive regulation of insulin secretion
cellular response to insulin stimulus
negative regulation of kinase activity
phosphatidylinositol-3-phosphate biosynthetic process
positive regulation of GTPase activity
positive regulation of glycogen biosynthetic process
positive regulation of glucose import
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
lipid homeostasis
cellular response to glucose stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support