Invitrogen

ISCA1 Polyclonal Antibody

2 Published Figures

View all (4) ISCA1 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (2)
Published Figures (2)

ISCA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ISCA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ISCA1 Antibody (PA5-60121) in IHC (P)

Immunohistochemical analysis of ISCA1 in human liver using ISCA1 Polyclonal Antibody (Product # PA5-60121) shows strong cytoplasmic positivity in hepatocytes.

ISCA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ISCA1 Antibody in Western Blot (WB)

ISCA1 Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P))

ISCA1 Antibody in Western Blot (WB)

Product Image Gallery

ISCA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ISCA1 in human liver using ISCA1 Polyclonal Antibody (Product # PA5-60121) shows strong cytoplasmic positivity in hepatocytes.

ISCA1 Antibody in Western Blot (WB) — Western blot analysis of ISCA1 in control (vector only transfected HEK293T lysate) and ISCA1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a ISCA1 Polyclonal Antibody (Product # PA5-60121).

ISCA1 Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P)) — 1 FIGURE Generation of neuron-specific Isca1 knockout rats. (A) The expression of ISCA1 in a brain section from a WT rat detected by immunofluorescence staining. DAPI (blue) stained the nucleus, and TRITC (red) conjugated secondary antibody was used to detect the ISCA1-specific antibodies. Scale bar: 1000 mum. (B) PCR was used for the genotyping of Isca1 CKO rats. Among the 12 littermates, numbers 2 and 6 are homozygote, 5 and 9 are wild types, and 3 is heterozygote. (C) Schematic diagram describing the strategy for generating CKO rats. (D) Western blots using an ISCA1-specific antibody showed the absence of ISCA1 protein in the brain tissues of CKO rats. GAPDH was used as a loading control. (E) Representative images showed the absence of ISCA1 protein in different regions of WT and CKO rats by IHC staining at the eighth week (S1, primary somatosensory cortex; DG, dentate gyrus). Scale bar for overview, 1000 mum; scale bar for magnified view; 50 mum. (F) Body weights compared between WT and CKO rats. (G) A representative graph shows the appearance of WT and CKO rats at the eighth week. (H) Survival analysis for WT and CKO rats; n = 17/group.

ISCA1 Antibody in Western Blot (WB) — FIGURE 1 The expression of ISCA1 under cardiomyopathy and establishment of myocardium specific Isca1 knockout heterozygote (HET) rat. (A) Phenotypic characteristics of clinical patients with Isca1 mutation. (B) ISCA1 expression in myocardium from ischemic and non-ischemic cardiomyopathy patients compared with normal donors ( p < .01, data from GSE1869 , n = 6 in normal group; n = 10 in ischemic group; n = 21 in non-ischemic group). (C) The HET ( Isca1 flox/+ /alpha-MHC-Cre ) rat was obtained by crossing Isca1 -floxed rats with alpha-MHC-Cre rats. Genotyping assays were performed using PCR (D) and Isca1 expression in the myocardium of HET rats was detected using western blot (E-F, n = 3 rats per group, * p < .05, vs. WT)

Product Details

PA5-60121

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human ISCA1. Recombinant protein control fragment (Product #RP-100140).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2642786

Product Specific Information

Immunogen sequence: DKLSSEFVFN NPNIKGTCGC GESFNI

Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 96%.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A neuron-specific Isca1 knockout rat developments multiple mitochondrial dysfunction syndromes.

Animal models and experimental medicine

Sheng H,Lu D,Qi X,Ling Y,Li J,Zhang X,Dong W,Chen W,Gao S,Gao X,Zhang L,Zhang L

PA5-60121 was used in Western Blot to investigate its pathological features and neuronal death.

Sat Apr 01 00:00:00 UTC 2023

A neuron-specific Isca1 knockout rat developments multiple mitochondrial dysfunction syndromes.

Animal models and experimental medicine

Sheng H,Lu D,Qi X,Ling Y,Li J,Zhang X,Dong W,Chen W,Gao S,Gao X,Zhang L,Zhang L

PA5-60121 was used in Western Blot to investigate its pathological features and neuronal death.

Sat Apr 01 00:00:00 UTC 2023

A neuron-specific Isca1 knockout rat developments multiple mitochondrial dysfunction syndromes.

Animal models and experimental medicine

Sheng H,Lu D,Qi X,Ling Y,Li J,Zhang X,Dong W,Chen W,Gao S,Gao X,Zhang L,Zhang L

PA5-60121 was used in Western Blot to investigate its pathological features and neuronal death.

Sat Apr 01 00:00:00 UTC 2023

Novel rat model of multiple mitochondrial dysfunction syndromes (MMDS) complicated with cardiomyopathy.

Animal models and experimental medicine

Ling Y,Ma J,Qi X,Zhang X,Kong Q,Guan F,Dong W,Chen W,Gao S,Gao X,Pan S,Ma Y,Lu D,Zhang L

PA5-60121 was used in Western Blotting to establish a rat model of MMDS complicated with cardiomyopathy which can be applied to the study of the mechanism of energy metabolism in cardiovascular diseases, as well as research and development of drugs.

Wed Dec 01 00:00:00 UTC 2021

Novel rat model of multiple mitochondrial dysfunction syndromes (MMDS) complicated with cardiomyopathy.

Animal models and experimental medicine

Ling Y,Ma J,Qi X,Zhang X,Kong Q,Guan F,Dong W,Chen W,Gao S,Gao X,Pan S,Ma Y,Lu D,Zhang L

PA5-60121 was used in Western Blotting to establish a rat model of MMDS complicated with cardiomyopathy which can be applied to the study of the mechanism of energy metabolism in cardiovascular diseases, as well as research and development of drugs.

Wed Dec 01 00:00:00 UTC 2021

Expanding the phenotype of mitochondrial disease: Novel pathogenic variant in ISCA1 leading to instability of the iron-sulfur cluster in the protein.

Mitochondrion

Lebigot E,Hully M,Amazit L,Gaignard P,Michel T,Rio M,Lombès M,Thérond P,Boutron A,Golinelli-Cohen MP

PA5-60121 was used in Western Blotting to report a patient carrying a novel pathogenic variant p.(Tyr101Cys) in ISCA1 leading to MMDS type 5.

Fri May 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: HESB like domain containing 2; HESB-like domain-containing protein 2; hIscA; iron sulfur assembly protein IscA; Iron-sulfur assembly protein IscA; Iron-sulfur cluster assembly 1 homolog, mitochondrial

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Gene Aliases: GK004; HBLD2; hIscA; ISA1; ISCA1

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UniProt ID: (Human) Q9BUE6

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Entrez Gene ID: (Human) 81689

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structural molecule activity ferrous iron binding 2 iron, 2 sulfur cluster binding 4 iron, 4 sulfur cluster binding

sulfur compound metabolic process iron-sulfur cluster assembly protein maturation by iron-sulfur cluster transfer

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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