Proteintech

ISCU Polyclonal Antibody

ISCU Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

ISCU Antibody (14812-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells, using ISCU antibody 14812-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

ISCU Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ISCU Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14812-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ISCU Fusion Protein Ag6562 (1-167 aa encoded by BC061903)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Two ISCU isoforms, ISCU1 (cytosolic, 15 kDa) and ISCU2 (mitochondrial, 18 kDa), were produced by alternative splicing. ISCU2 is considered the canonical isoform 1. Proteintech's ISCU antibody 14812-1-AP is raised against the full-length ISCU2 and is expected to detect 14, 15, and 18-19 kDa bands.

Immunogen sequence: MAAAGAFRL RRAASALLLR SPRLPARELS APARLYHKKV VDHYENPRNV GSLDKTSKNV GTGLVGAPAC GDVMKLQIQV DEKGKIVDAR FKTFGCGSAI ASSSLATEWV KGKTVEEALT IKNTDIAKEL CLPPVKLHCS MLAEDAIKAA LADYKLKQEP KKGEAEKK (1-167 aa encoded by BC061903)

Target Information

Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A mitochondrial iron-responsive pathway regulated by DELE1.

Molecular cell

Sekine Y,Houston R,Eckl EM,Fessler E,Narendra DP,Jae LT,Sekine S

Thu Jun 15 00:00:00 UTC 2023

Ferrodifferentiation regulates neurodevelopment via ROS generation.

Science China. Life sciences

Chang S,Wang P,Han Y,Ma Q,Liu Z,Zhong S,Lu Y,Chen R,Sun L,Wu Q,Gao G,Wang X,Chang YZ

Tue Aug 01 00:00:00 UTC 2023

Salidroside inhibits doxorubicin-induced cardiomyopathy by modulating a ferroptosis-dependent pathway.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Chen H,Zhu J,Le Y,Pan J,Liu Y,Liu Z,Wang C,Dou X,Lu D

Sun May 01 00:00:00 UTC 2022

Frataxin controls ketone body metabolism through regulation of OXCT1.

PNAS nexus

Dong YN,Mesaros C,Xu P,Mercado-Ayón E,Halawani S,Ngaba LV,Warren N,Sleiman P,Rodden LN,Schadt KA,Blair IA,Lynch DR

Fri Jul 01 00:00:00 UTC 2022

Skeletal muscle MiR-210 expression is associated with mitochondrial function in peripheral artery disease patients.

Translational research : the journal of laboratory and clinical medicine

Ismaeel A,Fletcher E,Miserlis D,Wechsler M,Papoutsi E,Haynatzki G,Smith RS,Bohannon WT,Koutakis P

Mon Aug 01 00:00:00 UTC 2022

Quercetin Antagonizes Glucose Fluctuation Induced Renal Injury by Inhibiting Aerobic Glycolysis via HIF-1α/miR-210/ISCU/FeS Pathway.

Frontiers in medicine

Xu WL,Liu S,Li N,Ye LF,Zha M,Li CY,Zhao Y,Pu Q,Bao JJ,Chen XJ,Yu JY,Pei YH

Tue Mar 23 00:00:00 UTC 2021

Macrophage miR-210 induction and metabolic reprogramming in response to pathogen interaction boost life-threatening inflammation.

Science advances

Virga F,Cappellesso F,Stijlemans B,Henze AT,Trotta R,Van Audenaerde J,Mirchandani AS,Sanchez-Garcia MA,Vandewalle J,Orso F,Riera-Domingo C,Griffa A,Ivan C,Smits E,Laoui D,Martelli F,Langouche L,Van den Berghe G,Feron O,Ghesquière B,Prenen H,Libert C,Walmsley SR,Corbet C,Van Ginderachter JA,Ivan M,Taverna D,Mazzone M

Sat May 01 00:00:00 UTC 2021

Rats with a Human Mutation of NFU1 Develop Pulmonary Hypertension.

American journal of respiratory cell and molecular biology

Niihori M,Eccles CA,Kurdyukov S,Zemskova M,Varghese MV,Stepanova AA,Galkin A,Rafikov R,Rafikova O

Sat Feb 01 00:00:00 UTC 2020

MicroRNA-210 Downregulates ISCU and Induces Mitochondrial Dysfunction and Neuronal Death in Neonatal Hypoxic-Ischemic Brain Injury.

Molecular neurobiology

Ma Q,Dasgupta C,Li Y,Huang L,Zhang L

Thu Aug 01 00:00:00 UTC 2019

MicroRNA-210 Downregulates ISCU and Induces Mitochondrial Dysfunction and Neuronal Death in Neonatal Hypoxic-Ischemic Brain Injury.

Molecular neurobiology

Ma Q,Dasgupta C,Li Y,Huang L,Zhang L

Thu Aug 01 00:00:00 UTC 2019

GRP75 overexpression rescues frataxin deficiency and mitochondrial phenotypes in Friedreich ataxia cellular models.

Human molecular genetics

Dong YN,McMillan E,Clark EM,Lin H,Lynch DR

Wed May 15 00:00:00 UTC 2019

GRP75 overexpression rescues frataxin deficiency and mitochondrial phenotypes in Friedreich ataxia cellular models.

Human molecular genetics

Dong YN,McMillan E,Clark EM,Lin H,Lynch DR

Wed May 15 00:00:00 UTC 2019

Phosphoinositide-3-kinase inhibition elevates ferritin level resulting depletion of labile iron pool and blocking of glioma cell proliferation.

Biochimica et biophysica acta. General subjects

Gupta P,Singh P,Pandey HS,Seth P,Mukhopadhyay CK

Fri Mar 01 00:00:00 UTC 2019

Phosphoinositide-3-kinase inhibition elevates ferritin level resulting depletion of labile iron pool and blocking of glioma cell proliferation.

Biochimica et biophysica acta. General subjects

Gupta P,Singh P,Pandey HS,Seth P,Mukhopadhyay CK

Fri Mar 01 00:00:00 UTC 2019

NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.

Cell research

Hou T,Zhang R,Jian C,Ding W,Wang Y,Ling S,Ma Q,Hu X,Cheng H,Wang X

Sun Sep 01 00:00:00 UTC 2019

NDUFAB1 protects against obesity and insulin resistance by enhancing mitochondrial metabolism.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang R,Hou T,Cheng H,Wang X

Sun Dec 01 00:00:00 UTC 2019

The Favored Mechanism for Coping with Acute Cold Stress: Upregulation of miR-210 in Rats.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Guo W,Lian S,Zhen L,Zang S,Chen Y,Lang L,Xu B,Guo J,Ji H,Wang J,Fu S,Zhang L,Yang H

Wed Aug 01 00:00:00 UTC 2018

The Favored Mechanism for Coping with Acute Cold Stress: Upregulation of miR-210 in Rats.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Guo W,Lian S,Zhen L,Zang S,Chen Y,Lang L,Xu B,Guo J,Ji H,Wang J,Fu S,Zhang L,Yang H

Wed Aug 01 00:00:00 UTC 2018

Iron accumulation in senescent cells is coupled with impaired ferritinophagy and inhibition of ferroptosis.

Redox biology

Masaldan S,Clatworthy SAS,Gamell C,Meggyesy PM,Rigopoulos AT,Haupt S,Haupt Y,Denoyer D,Adlard PA,Bush AI,Cater MA

Sun Apr 01 00:00:00 UTC 2018

Iron accumulation in senescent cells is coupled with impaired ferritinophagy and inhibition of ferroptosis.

Redox biology

Masaldan S,Clatworthy SAS,Gamell C,Meggyesy PM,Rigopoulos AT,Haupt S,Haupt Y,Denoyer D,Adlard PA,Bush AI,Cater MA

Sun Apr 01 00:00:00 UTC 2018

Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation.

PloS one

Ferecatu I,Canal F,Fabbri L,Mazure NM,Bouton C,Golinelli-Cohen MP

Mon Jul 09 00:00:00 UTC 2018

Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation.

PloS one

Ferecatu I,Canal F,Fabbri L,Mazure NM,Bouton C,Golinelli-Cohen MP

Mon Jul 09 00:00:00 UTC 2018

Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation.

PloS one

Ferecatu I,Canal F,Fabbri L,Mazure NM,Bouton C,Golinelli-Cohen MP

Mon Jul 09 00:00:00 UTC 2018

Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation.

PloS one

Ferecatu I,Canal F,Fabbri L,Mazure NM,Bouton C,Golinelli-Cohen MP

Mon Jul 09 00:00:00 UTC 2018

Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.

Scientific reports

Wu L,Guo X,Lyu Y,Clarke SL,Lucas EA,Smith BJ,Hildebrand D,Wang W,Medeiros DM,Shen X,Lin D

Tue Nov 07 00:00:00 UTC 2017

Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.

Scientific reports

Wu L,Guo X,Lyu Y,Clarke SL,Lucas EA,Smith BJ,Hildebrand D,Wang W,Medeiros DM,Shen X,Lin D

Tue Nov 07 00:00:00 UTC 2017

Regulation of iron homeostasis by the p53-ISCU pathway.

Scientific reports