Invitrogen

ISG15 Recombinant Rabbit Monoclonal Antibody (1H9L21)

ISG15 Antibody in Immunocytochemistry (ICC/IF)

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ISG15 Antibody (703132) in ICC/IF

For immunofluorescence analysis, RAW 264.7 cells were fixed and permeabilized for detection of endogenous ISG15 (Mouse) using Anti-ISG15 (Mouse) Recombinant Rabbit Monoclonal Antibody (Product # 703132, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a... View More

ISG15 Antibody in Mass Spectrometry (MS)

Product Image Gallery

ISG15 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. NIH/3T3 cells were treated with IFN-beta (1000 U/mL for 24 hrs) and were transfected with ISG15 Mouse siRNA and a decrease in signal intensity was observed in Western blot application using Anti-ISG15 (Mouse) Recombinant Rabbit Monoclonal Antibody (Product # 703132). {KD}

Product Details

703132

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100

Mass Spectrometry (MS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

1H9L21

Immunogen

Protein corresponding to mouse ISG15 [aa2-aa155]

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2784563

Product Specific Information

This antibody is predicted to react with Rat, Cat.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega, but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog. Mutations affecting the gene can result in Immunodeficiency 38, with basal ganglia calcification (IMD38).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pressure overload induces ISG15 to facilitate adverse ventricular remodeling and promote heart failure.

The Journal of clinical investigation

Yerra VG,Batchu SN,Kaur H,Kabir MDG,Liu Y,Advani SL,Tran DT,Sadeghian S,Sedrak P,Billia F,Kuzmanov U,Gramolini AO,Qasrawi DO,Petrotchenko EV,Borchers CH,Connelly KA,Advani A

703132 was used in Western Blot to identify the myofibrillar protein filamin-C as an ISGylation target.

Mon May 01 00:00:00 UTC 2023

Pressure overload induces ISG15 to facilitate adverse ventricular remodeling and promote heart failure.

The Journal of clinical investigation

Yerra VG,Batchu SN,Kaur H,Kabir MDG,Liu Y,Advani SL,Tran DT,Sadeghian S,Sedrak P,Billia F,Kuzmanov U,Gramolini AO,Qasrawi DO,Petrotchenko EV,Borchers CH,Connelly KA,Advani A

703132 was used in Western Blot to identify the myofibrillar protein filamin-C as an ISGylation target.

Mon May 01 00:00:00 UTC 2023

Pressure overload induces ISG15 to facilitate adverse ventricular remodeling and promote heart failure.

The Journal of clinical investigation

Yerra VG,Batchu SN,Kaur H,Kabir MDG,Liu Y,Advani SL,Tran DT,Sadeghian S,Sedrak P,Billia F,Kuzmanov U,Gramolini AO,Qasrawi DO,Petrotchenko EV,Borchers CH,Connelly KA,Advani A

703132 was used in Western Blot to identify the myofibrillar protein filamin-C as an ISGylation target.

Mon May 01 00:00:00 UTC 2023

Non-muscle myosins control radial glial basal endfeet to mediate interneuron organization.

PLoS biology

D'Arcy BR,Lennox AL,Manso Musso C,Bracher A,Escobar-Tomlienovich C,Perez-Sanchez S,Silver DL

703132 was used in Immunohistochemistry to demonstrate the utility of in vivo proximity labeling for dissecting local control of complex systems and reveals new mechanisms for dictating RGC integrity and cortical architecture.

Wed Feb 01 00:00:00 UTC 2023

In Vivo Profiling of Individual Multiciliated Cells during Acute Influenza A Virus Infection.

Journal of virology

Hamele CE,Russell AB,Heaton NS

703132 was used in Immunohistochemistry (Frozen) to propose that different ratios of these individual cellular responses may contribute to the broader antiviral state of the lung and may ultimately affect disease severity.

Wed Jul 27 00:00:00 UTC 2022

CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive states.

Nature neuroscience

Leng K,Rose IVL,Kim H,Xia W,Romero-Fernandez W,Rooney B,Koontz M,Li E,Ao Y,Wang S,Krawczyk M,Tcw J,Goate A,Zhang Y,Ullian EM,Sofroniew MV,Fancy SPJ,Schrag MS,Lippmann ES,Kampmann M

703132 was used in Immunohistochemistry (Frozen) to find that autocrine-paracrine IL-6 and interferon signaling downstream of canonical NF-κB activation drove two distinct inflammatory reactive signatures, one promoted by STAT3 and the other inhibited by STAT3.

Tue Nov 01 00:00:00 UTC 2022

Single-Cell RNA Sequencing Reveals the Temporal Diversity and Dynamics of Cardiac Immunity after Myocardial Infarction.

Small methods

Jin K,Gao S,Yang P,Guo R,Li D,Zhang Y,Lu X,Fan G,Fan X

703132 was used in Immunohistochemistry to provide a basis for developing cell type- and time-specific interventions in MI.

Tue Mar 01 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: interferon, alpha-inducible protein; Interferon-induced 15 kDa protein; interferon-induced 15-KDa protein; Interferon-induced 17 kDa protein; interferon-stimulated protein (15 kDa); interferon-stimulated protein 15; IP17; Ubiquitin cross-reactive protein; Ubiquitin-like protein ISG15

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Gene Aliases: G1p2; IGI15; IP17; Irfp; Isg15; UCRP

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UniProt ID: (Mouse) Q64339

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Entrez Gene ID: (Mouse) 100038882

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Function(s)

protein binding protein tag

Process(es)

modification-dependent protein catabolic process negative regulation of protein ubiquitination ISG15-protein conjugation regulation of interferon-gamma production response to type I interferon defense response to bacterium negative regulation of viral genome replication positive regulation of erythrocyte differentiation defense response to virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ISG15 Recombinant Rabbit Monoclonal Antibody (1H9L21)

ISG15 Antibody (703132) in ICC/IF

Product Details

703132

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Mass Spectrometry (MS)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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