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Invitrogen
ISG15 Recombinant Rabbit Monoclonal Antibody (7H29L24)
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FIGURE: 1 / 7
ISG15 Antibody (703131) in WB
Immunoblot with monoclonal anti-ISG15 antibody 7H29L24 (Product # 703131). NK-92, HeLa, Jurkat, and A549 cells were either untreated or IFN-b-treated for 48 hours as indicated. 8220Transfected8221 (last lane) represents extract from HEK293T cells transfected with plasmids expressing 3X-FLAG-ISG15, UBE1L, UBCH8, and HERC5. Cells were lysed in 1% NP40 lysis buffer (50 mM Tris pH 8, 150 mM NaCl, 1% NP-40) with PMSF, aprotinin, leupeptin, and 1mM DTT. 30 mg total protein per sample were loaded onto a 13% Tris-Glycine PAGE gel, transferred to n... View More
Product Details
703131
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
7H29L24
Immunogen
Protein corresponding to human ISG15 [aa2-aa155]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2784562
Product Specific Information
This antibody is predicted to react with Monkey, Dog, Cat, Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega, but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog. Mutations affecting the gene can result in Immunodeficiency 38, with basal ganglia calcification (IMD38).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: hUCRP; interferon, alpha-inducible protein (clone IFI-15K); Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; interferon-induced 17-kDa/15-kDa protein; interferon-stimulated protein, 15 kDa; IP17; Ubiquitin cross-reactive protein; Ubiquitin-like protein ISG15
Gene Aliases: G1P2; hUCRP; IFI15; IMD38; IP17; ISG15; UCRP
UniProt ID: (Human) P05161
Entrez Gene ID: (Human) 9636
modification-dependent protein catabolic process
translesion synthesis
negative regulation of protein ubiquitination
ISG15-protein conjugation
negative regulation of type I interferon production
regulation of interferon-gamma production
response to type I interferon
defense response to bacterium
negative regulation of viral genome replication
positive regulation of erythrocyte differentiation
defense response to virus
type I interferon signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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