Proteintech

IST1 Polyclonal Antibody

IST1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

IST1 Antibody (51002-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using KIAA0174 antibody 51002-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

IST1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

51002-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:8,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 12 publications 12 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IST1 Fusion Protein Ag0721 (1-364 aa encoded by BC000430)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MLGSGFKAE RLRVNLRLVI NRLKLLEKKK TELAQKARKE IADYLAAGKD ERARIRVEHI IREDYLVEAM EILELYCDLL LARFGLIQSM KELDSGLAES VSTLIWAAPR LQSEVAELKI VADQLCAKYS KEYGKLCRTN QIGTVNDRLM HKLSVEAPPK ILVERYLIEI AKNYNVPYEP DSVVMAEAPP GVETDLIDVG FTDDVKKGGP GRGGSGGFTA PVGGPDGTVP MPMPMPMPMP SANTPFSYPL PKGPSDFNGL PMGTYQAFPN IHPPQIPATP PSYESMTLML IRISLLHRLL VLDPSQKPLQ SFLPDLQITM TTLSYQSCHL CQTHYQLHLL VPAPQHLKTL TLMIFPGGLK S (1-364 aa encoded by BC000430)

Target Information

Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (ESCRT) protein.

Virologica Sinica

Shen W,Liu C,Hu Y,Ding Q,Feng J,Liu Z,Kong X

Thu Jun 01 00:00:00 UTC 2023

V. cholerae MakA is a cholesterol-binding pore-forming toxin that induces non-canonical autophagy.

The Journal of cell biology

Jia X,Knyazeva A,Zhang Y,Castro-Gonzalez S,Nakamura S,Carlson LA,Yoshimori T,Corkery DP,Wu YW

Mon Dec 05 00:00:00 UTC 2022

AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody.

PLoS genetics

Merigliano C,Burla R,La Torre M,Del Giudice S,Teo H,Liew CW,Chojnowski A,Goh WI,Olmos Y,Maccaroni K,Giubettini M,Chiolo I,Carlton JG,Raimondo D,Vernì F,Stewart CL,Rhodes D,Wright GD,Burke BE,Saggio I

Sun Aug 01 00:00:00 UTC 2021

CDK1 controls CHMP7-dependent nuclear envelope reformation.

eLife

Gatta AT,Olmos Y,Stoten CL,Chen Q,Rosenthal PB,Carlton JG

Wed Jul 21 00:00:00 UTC 2021

CDK1 controls CHMP7-dependent nuclear envelope reformation.

eLife

Gatta AT,Olmos Y,Stoten CL,Chen Q,Rosenthal PB,Carlton JG

Wed Jul 21 00:00:00 UTC 2021

De Novo VPS4A Mutations Cause Multisystem Disease with Abnormal Neurodevelopment.

American journal of human genetics

Rodger C,Flex E,Allison RJ,Sanchis-Juan A,Hasenahuer MA,Cecchetti S,French CE,Edgar JR,Carpentieri G,Ciolfi A,Pantaleoni F,Bruselles A,Onesimo R,Zampino G,Marcon F,Siniscalchi E,Lees M,Krishnakumar D,McCann E,Yosifova D,Jarvis J,Kruer MC,Marks W,Campbell J,Allen LE,Gustincich S,Raymond FL,Tartaglia M,Reid E

Thu Dec 03 00:00:00 UTC 2020

ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic.

Cellular and molecular life sciences : CMLS

Connell JW,Allison RJ,Rodger CE,Pearson G,Zlamalova E,Reid E

Wed Jul 01 00:00:00 UTC 2020

Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High-Resolution Imaging Techniques SIM and Cryo-SXT.

Methods in molecular biology (Clifton, N.J.)

Adar-Levor S,Goliand I,Elbaum M,Elia N

Mon Mar 02 00:00:00 UTC 2020

Regulation of OLC1 protein expression by the anaphase-promoting complex.

Oncology letters

Zhang X,Chen W,Yin N,Dong L,Fu M,Zhan Q,Tong T

Fri Mar 01 00:00:00 UTC 2019

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Nature

Liu S,Kwon M,Mannino M,Yang N,Renda F,Khodjakov A,Pellman D

Sat Sep 01 00:00:00 UTC 2018

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Nature

Liu S,Kwon M,Mannino M,Yang N,Renda F,Khodjakov A,Pellman D

Sat Sep 01 00:00:00 UTC 2018

VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.

Scientific reports

Ott C,Nachmias D,Adar S,Jarnik M,Sherman S,Birnbaum RY,Lippincott-Schwartz J,Elia N

Tue Feb 20 00:00:00 UTC 2018

VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.

Scientific reports

Ott C,Nachmias D,Adar S,Jarnik M,Sherman S,Birnbaum RY,Lippincott-Schwartz J,Elia N

Tue Feb 20 00:00:00 UTC 2018

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

Cell reports

Goliand I,Adar-Levor S,Segal I,Nachmias D,Dadosh T,Kozlov MM,Elia N

Tue Aug 14 00:00:00 UTC 2018

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

Cell reports

Goliand I,Adar-Levor S,Segal I,Nachmias D,Dadosh T,Kozlov MM,Elia N

Tue Aug 14 00:00:00 UTC 2018

Defects in ER-endosome contacts impact lysosome function in hereditary spastic paraplegia.

The Journal of cell biology

Allison R,Edgar JR,Pearson G,Rizo T,Newton T,Günther S,Berner F,Hague J,Connell JW,Winkler J,Lippincott-Schwartz J,Beetz C,Winner B,Reid E

Mon May 01 00:00:00 UTC 2017

Defects in ER-endosome contacts impact lysosome function in hereditary spastic paraplegia.

The Journal of cell biology

Allison R,Edgar JR,Pearson G,Rizo T,Newton T,Günther S,Berner F,Hague J,Connell JW,Winkler J,Lippincott-Schwartz J,Beetz C,Winner B,Reid E

Mon May 01 00:00:00 UTC 2017

ALIX and ESCRT-I/II function as parallel ESCRT-III recruiters in cytokinetic abscission.

The Journal of cell biology

Christ L,Wenzel EM,Liestøl K,Raiborg C,Campsteijn C,Stenmark H

Mon Feb 29 00:00:00 UTC 2016

ALIX and ESCRT-I/II function as parallel ESCRT-III recruiters in cytokinetic abscission.

The Journal of cell biology

Christ L,Wenzel EM,Liestøl K,Raiborg C,Campsteijn C,Stenmark H

Mon Feb 29 00:00:00 UTC 2016

An Automated Image Analysis System to Quantify Endosomal Tubulation.

PloS one

Newton TM,Reid E

Tue Jul 18 00:00:00 UTC 2017

An Automated Image Analysis System to Quantify Endosomal Tubulation.

PloS one

Newton TM,Reid E

Tue Jul 18 00:00:00 UTC 2017

Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.

Nature

Vietri M,Schink KO,Campsteijn C,Wegner CS,Schultz SW,Christ L,Thoresen SB,Brech A,Raiborg C,Stenmark H

Thu Jun 11 00:00:00 UTC 2015

Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.

Nature

Vietri M,Schink KO,Campsteijn C,Wegner CS,Schultz SW,Christ L,Thoresen SB,Brech A,Raiborg C,Stenmark H

Thu Jun 11 00:00:00 UTC 2015

Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.

Nature

Vietri M,Schink KO,Campsteijn C,Wegner CS,Schultz SW,Christ L,Thoresen SB,Brech A,Raiborg C,Stenmark H

Thu Jun 11 00:00:00 UTC 2015

Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.

Nature

Vietri M,Schink KO,Campsteijn C,Wegner CS,Schultz SW,Christ L,Thoresen SB,Brech A,Raiborg C,Stenmark H

Thu Jun 11 00:00:00 UTC 2015

Over-expression of the overexpressed in lung cancer-1 is associated with poor prognosis in colorectal cancer.

Anticancer research

Liu J,Song H,Yao L,Liu Y,Zhang Y,Zhao H,Ji H,Wang Y

Wed Jan 01 00:00:00 UTC 2014

An ESCRT-spastin interaction promotes fission of recycling tubules from the endosome.

The Journal of cell biology

Allison R,Lumb JH,Fassier C,Connell JW,Ten Martin D,Seaman MN,Hazan J,Reid E

Mon Aug 05 00:00:00 UTC 2013

An ESCRT-spastin interaction promotes fission of recycling tubules from the endosome.

The Journal of cell biology

Allison R,Lumb JH,Fassier C,Connell JW,Ten Martin D,Seaman MN,Hazan J,Reid E

Mon Aug 05 00:00:00 UTC 2013

Over-expression of the overexpressed in lung cancer 1 is associated with poor prognosis in epithelial ovarian cancer.

Journal of surgical oncology

Jia C,Li X,Sun H,Sui L

Sat Jun 01 00:00:00 UTC 2013

Over-expression of the overexpressed in lung cancer 1 is associated with poor prognosis in epithelial ovarian cancer.

Journal of surgical oncology

Jia C,Li X,Sun H,Sui L

Sat Jun 01 00:00:00 UTC 2013

Over-expression of the overexpressed in lung cancer 1 is associated with poor prognosis in epithelial ovarian cancer.

Journal of surgical oncology

Jia C,Li X,Sun H,Sui L

Sat Jun 01 00:00:00 UTC 2013

Over-expression of the overexpressed in lung cancer 1 is associated with poor prognosis in epithelial ovarian cancer.

Journal of surgical oncology

Jia C,Li X,Sun H,Sui L

Sat Jun 01 00:00:00 UTC 2013

ESCRT-III binding protein MITD1 is involved in cytokinesis and has an unanticipated PLD fold that binds membranes.

Proceedings of the National Academy of Sciences of the United States of America

Hadders MA,Agromayor M,Obita T,Perisic O,Caballe A,Kloc M,Lamers MH,Williams RL,Martin-Serrano J

Tue Oct 23 00:00:00 UTC 2012

ESCRT-III binding protein MITD1 is involved in cytokinesis and has an unanticipated PLD fold that binds membranes.

Proceedings of the National Academy of Sciences of the United States of America

Hadders MA,Agromayor M,Obita T,Perisic O,Caballe A,Kloc M,Lamers MH,Williams RL,Martin-Serrano J

Tue Oct 23 00:00:00 UTC 2012

The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain.

Structure (London, England : 1993)

Agromayor M,Soler N,Caballe A,Kueck T,Freund SM,Allen MD,Bycroft M,Perisic O,Ye Y,McDonald B,Scheel H,Hofmann K,Neil SJ,Martin-Serrano J,Williams RL

Wed Mar 07 00:00:00 UTC 2012

The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain.

Structure (London, England : 1993)

Agromayor M,Soler N,Caballe A,Kueck T,Freund SM,Allen MD,Bycroft M,Perisic O,Ye Y,McDonald B,Scheel H,Hofmann K,Neil SJ,Martin-Serrano J,Williams RL

Wed Mar 07 00:00:00 UTC 2012

Cigarette smoke suppresses the ubiquitin-dependent degradation of OLC1.

Biochemical and biophysical research communications

Zhang X,Xiao T,Cheng S,Tong T,Gao Y

Fri Apr 22 00:00:00 UTC 2011

Essential role of hIST1 in cytokinesis.

Molecular biology of the cell

Agromayor M,Carlton JG,Phelan JP,Matthews DR,Carlin LM,Ameer-Beg S,Bowers K,Martin-Serrano J

Sun Mar 01 00:00:00 UTC 2009

Essential role of hIST1 in cytokinesis.

Molecular biology of the cell

Agromayor M,Carlton JG,Phelan JP,Matthews DR,Carlin LM,Ameer-Beg S,Bowers K,Martin-Serrano J

Sun Mar 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: Charged multivesicular body protein 8; CHMP8; hIST1; increased sodium tolerance 1 homolog; IST1 homolog; IST1, endosomal sorting complex required for transport-III component; MGC117220; overexpressed in lung cancer 1; pm28; Putative MAPK-activating protein PM28

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Gene Aliases: IST1; KIAA0174; OLC1

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UniProt ID: (Human) P53990

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Entrez Gene ID: (Human) 9798

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Function(s)

protein binding protein domain specific binding protein complex binding MIT domain binding cadherin binding involved in cell-cell adhesion

Process(es)

cytokinesis positive regulation of defense response to virus by host protein localization abscission protein transport viral release from host cell multivesicular body assembly establishment of protein localization positive regulation of proteolysis viral capsid secondary envelopment positive regulation of collateral sprouting cell division cell-cell adhesion mitophagy in response to mitochondrial depolarization xenophagy ESCRT III complex disassembly

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IST1 Polyclonal Antibody

IST1 Antibody (51002-1-AP) in ICC/IF

Product Details

51002-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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