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Invitrogen
IST1 Polyclonal Antibody
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FIGURE: 1 / 1
IST1 Antibody (PA5-97212) in WB
Western blot analysis of IST1 in extracts of various cell lines. Samples were incubated with IST1 Polyclonal antibody (Product # PA5-97212) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-97212
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human IST1 (NP_0012579061)
Conjugate
Form
Liquid
Concentration
1.87 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2809014
Product Specific Information
Immunogen sequence: MLGSGFKAER LRVNLRLVIN RLKLLEKKKT ELAQKARKEI ADYLAAGKDE RARIRVEHII REDYLVEAME ILELYCDLLL ARFGLIQSMK ELDSGLAESV STLIWAAPRL QSEVAELKIV ADQLCAKYSK EYGKLCRTNQ IGTVNDRLMH KLSVEAPPKI LVERYLIEIA KNYNVPYEPD SVVMAEAPPG VETDLIDVGF TDDVKKGGPG RGGSGGFTAP VGGPDGTVPM PMPMPMPMPS ANTPFSYPLP KGPVDDINAD KNISSAQIVG PGPKPEASAK LPSRPADNYD NFVLPELPSV PDTLPTASAG ASTSASEDID FDDLSRRFEE LKKKT; Positive Samples: A-549, HeLa, Mouse heart, Rat brain; Cellular Location: Cytoplasm, Cytoplasmic vesicle, Midbody, centrosome, cytoskeleton, microtubule organizing center
Target Information
Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Charged multivesicular body protein 8; CHMP8; hIST1; increased sodium tolerance 1 homolog; IST1 homolog; IST1, endosomal sorting complex required for transport-III component; MAPK activating protein PM28; MGC117220; overexpressed in lung cancer 1; pm28; Putative MAPK-activating protein PM28
Gene Aliases: 2400003C14Rik; AW536298; IST1; KIAA0174; mKIAA0174; OLC1; RGD1307799
UniProt ID: (Human) P53990, (Rat) Q568Z6, (Mouse) Q9CX00
Entrez Gene ID: (Human) 9798, (Rat) 307833, (Mouse) 71955
cytokinesis
positive regulation of defense response to virus by host
protein localization
abscission
protein transport
viral release from host cell
multivesicular body assembly
establishment of protein localization
positive regulation of proteolysis
viral capsid secondary envelopment
positive regulation of collateral sprouting
cell division
cell-cell adhesion
mitophagy in response to mitochondrial depolarization
xenophagy
ESCRT III complex disassembly
cell cycle
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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