Proteintech

ITCH Polyclonal Antibody

ITCH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ITCH Antibody (20920-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using (Product # 20920-1-AP) (ITCH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ITCH Antibody in Immunoprecipitation (IP)

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Product Details

20920-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ITCH Fusion Protein Ag15120 (556-903 aa encoded by BC011571)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: TLFEDSFQQ IMSFSPQDLR RRLWVIFPGE EGLDYGGVAR EWFFLLSHEV LNPMYCLFEY AGKDNYCLQI NPASYINPDH LKYFRFIGRF IAMALFHGKF IDTGFSLPFY KRILNKPVGL KDLESIDPEF YNSLIWVKEN NIEECDLEMY FSVDKEILGE IKSHDLKPNG GNILVTEENK EEYIRMVAEW RLSRGVEEQT QAFFEGFNEI LPQQYLQYFD AKELEVLLCG MQEIDLNDWQ RHAIYRHYAR TSKQIMWFWQ FVKEIDNEKR MRLLQFVTGT CRLPVGGFAD LMGSNGPQKF CIEKVGKENW LPRSHTCFNR LDLPPYKSYE QLKEKLLFAI EETEGFGQE (556-903 aa encoded by BC011571)

Target Information

Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Downregulation of TEX11 promotes S-Phase progression and proliferation in colorectal cancer cells through the FOXO3a/COP1/c-Jun/p21 axis.

Oncogene

Zhang X,Hu F,Zhu B,Jiao X,Li Y,Wu S,Ren G,Li J,Xie Q,Pan Y,Li H,Zhao L

Tue Nov 01 00:00:00 UTC 2022

Coiled-Coil Domain-Containing 68 Downregulation Promotes Colorectal Cancer Cell Growth by Inhibiting ITCH-Mediated CDK4 Degradation.

Frontiers in oncology

Wang C,Li H,Wu L,Jiao X,Jin Z,Zhu Y,Fang Z,Zhang X,Huang H,Zhao L

Thu May 13 00:00:00 UTC 2021

Coiled-Coil Domain-Containing 68 Downregulation Promotes Colorectal Cancer Cell Growth by Inhibiting ITCH-Mediated CDK4 Degradation.

Frontiers in oncology

Wang C,Li H,Wu L,Jiao X,Jin Z,Zhu Y,Fang Z,Zhang X,Huang H,Zhao L

Thu May 13 00:00:00 UTC 2021

Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.

Journal of molecular medicine (Berlin, Germany)

Zhang Y,Chen X,Wang Q,Du C,Lu W,Yuan H,Zhang Z,Li D,Ling X,Ren X,Zhao Y,Su Q,Xing Z,Qin Y,Yang X,Shen Y,Wu H,Qi Y

Wed Dec 01 00:00:00 UTC 2021

GRAMD4 inhibits tumour metastasis by recruiting the E3 ligase ITCH to target TAK1 for degradation in hepatocellular carcinoma.

Clinical and translational medicine

Ge QY,Chen J,Li GX,Tan XL,Song J,Ning D,Mo J,Du PC,Liu QM,Liang HF,Ding ZY,Zhang XW,Zhang BX

Mon Nov 01 00:00:00 UTC 2021

GRAMD4 inhibits tumour metastasis by recruiting the E3 ligase ITCH to target TAK1 for degradation in hepatocellular carcinoma.

Clinical and translational medicine

Ge QY,Chen J,Li GX,Tan XL,Song J,Ning D,Mo J,Du PC,Liu QM,Liang HF,Ding ZY,Zhang XW,Zhang BX

Mon Nov 01 00:00:00 UTC 2021

JAC1 suppresses proliferation of breast cancer through the JWA/p38/SMURF1/HER2 signaling.

Cell death discovery

Ren Y,Chen D,Zhai Z,Chen J,Li A,Liang Y,Zhou J

Mon Apr 19 00:00:00 UTC 2021

ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3.

Theranostics

Zhou L,Song Z,Hu J,Liu L,Hou Y,Zhang X,Yang X,Chen K

Wed Aug 04 00:00:00 UTC 2021

The E3 ubiquitin ligase Itch deficiency promotes antigen-driven B-cell responses in mice.

European journal of immunology

Fang Y,He Y,Zhai B,Hou C,Xu R,Xing C,Wang X,Ma N,Han G,Wang R

Fri Jan 01 00:00:00 UTC 2021

The E3 ubiquitin ligase Itch deficiency promotes antigen-driven B-cell responses in mice.

European journal of immunology

Fang Y,He Y,Zhai B,Hou C,Xu R,Xing C,Wang X,Ma N,Han G,Wang R

Fri Jan 01 00:00:00 UTC 2021

Heat stress activates YAP/TAZ to induce the heat shock transcriptome.

Nature cell biology

Luo M,Meng Z,Moroishi T,Lin KC,Shen G,Mo F,Shao B,Wei X,Zhang P,Wei Y,Guan KL

Tue Dec 01 00:00:00 UTC 2020

ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.

Developmental cell

Ran J,Liu M,Feng J,Li H,Ma H,Song T,Cao Y,Zhou P,Wu Y,Yang Y,Yang Y,Yu F,Guo H,Zhang L,Xie S,Li D,Gao J,Zhang X,Zhu X,Zhou J

Mon May 04 00:00:00 UTC 2020

Calhex₂₃₁ Alleviates High Glucose-Induced Myocardial Fibrosis via Inhibiting Itch-Ubiquitin Proteasome Pathway in Vitro.

Biological & pharmaceutical bulletin

Yuan H,Xu J,Xu X,Gao T,Wang Y,Fan Y,Hu J,Shao Y,Zhao B,Li H,Sun J,Xu C

Thu Aug 01 00:00:00 UTC 2019

Increased miR-214 expression suppresses cell migration and proliferation in Hirschsprung disease by interacting with PLAGL2.

Pediatric research

Wu L,Yuan W,Chen J,Zhou Z,Shu Y,Ji J,Liu Z,Tang Q,Zhang X,Shu X

Tue Oct 01 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: AIP4; atrophin-1 interacting protein 4; Atrophin-1-interacting protein 4; dJ468O1.1; E3 ubiquitin-protein ligase Itchy; E3 ubiquitin-protein ligase Itchy homolog; HECT-type E3 ubiquitin transferase Itchy homolog; Itch; itchy E3 ubiquitin protein ligase homolog; NAPP1; NFE2-associated polypeptide 1

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Gene Aliases: 6720481N21Rik; 8030492O04Rik; A130065M08; ADMFD; AIF4; AIP4; C230047C07Rik; ITCH; NAPP1

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UniProt ID: (Human) Q96J02, (Mouse) Q8C863

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Entrez Gene ID: (Human) 83737, (Mouse) 16396

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Function(s)

ubiquitin-protein transferase activity protein binding ligase activity ubiquitin-like protein transferase activity ribonucleoprotein complex binding CXCR chemokine receptor binding ubiquitin protein ligase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity

Process(es)

regulation of cell growth positive regulation of T cell anergy ubiquitin-dependent protein catabolic process apoptotic process inflammatory response Notch signaling pathway protein ubiquitination negative regulation of NF-kappaB transcription factor activity negative regulation of type I interferon production protein K29-linked ubiquitination protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process innate immune response positive regulation of protein catabolic process negative regulation of JNK cascade negative regulation of alpha-beta T cell proliferation viral entry into host cell negative regulation of defense response to virus defense response to virus nucleotide-binding oligomerization domain containing signaling pathway protein K63-linked ubiquitination protein K48-linked ubiquitination regulation of protein deubiquitination protein polyubiquitination immune system process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ITCH Polyclonal Antibody

ITCH Antibody (20920-1-AP) in IHC (P)

Product Details

20920-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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