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ITCH Antibody (MA5-29372) in ICC/IF
Immunofluorescence staining of Human ITCH in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with ITCH Monoclonal Antibody (1) (Product # MA5-29372, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Product Details
MA5-29372
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
01
Immunogen
Recombinant Human ITCH/AIP4 protein (Arg526-Glu903)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785246
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human ITCH/AIP4.
Target Information
Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AIP4; atrophin-1 interacting protein 4; Atrophin-1-interacting protein 4; dJ468O1.1; E3 ubiquitin-protein ligase Itchy homolog; HECT-type E3 ubiquitin transferase Itchy homolog; Itch; itchy E3 ubiquitin protein ligase homolog; NAPP1; NFE2-associated polypeptide 1
Gene Aliases: ADMFD; AIF4; AIP4; ITCH; NAPP1
UniProt ID: (Human) Q96J02
Entrez Gene ID: (Human) 83737
regulation of cell growth
positive regulation of T cell anergy
ubiquitin-dependent protein catabolic process
apoptotic process
inflammatory response
Notch signaling pathway
protein ubiquitination
negative regulation of NF-kappaB transcription factor activity
negative regulation of type I interferon production
protein K29-linked ubiquitination
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
innate immune response
positive regulation of protein catabolic process
negative regulation of JNK cascade
negative regulation of alpha-beta T cell proliferation
viral entry into host cell
negative regulation of defense response to virus
defense response to virus
nucleotide-binding oligomerization domain containing signaling pathway
protein K63-linked ubiquitination
protein K48-linked ubiquitination
regulation of protein deubiquitination
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