Invitrogen

ITCH Recombinant Rabbit Monoclonal Antibody (7I7B10)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-38170

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

7I7B10

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ITCH (Q96J02)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.6 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2898087

Product Specific Information

Positive Samples: HeLa, 293T, Raji, NIH/3T3, C6, Mouse lung

Immunogen sequence: MSDSGSQLGS MGSLTMKSQL QITVISAKLK ENKKNWFGPS PYVEVTVDGQ SKKTEKCNNT NSPKWKQPLT VIVTPVSKLH FRVWSHQTLK SDVLLGTAAL

Target Information

Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AIP4; atrophin-1 interacting protein 4; Atrophin-1-interacting protein 4; dJ468O1.1; E3 ubiquitin-protein ligase Itchy; E3 ubiquitin-protein ligase Itchy homolog; HECT-type E3 ubiquitin transferase Itchy homolog; Itch; itch E3 ubiquitin ligase; itchy E3 ubiquitin protein ligase homolog; itchy, E3 ubiquitin protein ligase; NAPP1; NFE2-associated polypeptide 1

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Gene Aliases: 6720481N21Rik; 8030492O04Rik; A130065M08; ADMFD; AIF4; AIP4; C230047C07Rik; ITCH; NAPP1

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UniProt ID: (Human) Q96J02, (Mouse) Q8C863

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Entrez Gene ID: (Human) 83737, (Mouse) 16396, (Rat) 311567

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Function(s)
ubiquitin-protein transferase activity protein binding ligase activity ubiquitin-like protein transferase activity ribonucleoprotein complex binding CXCR chemokine receptor binding ubiquitin protein ligase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity
Process(es)
regulation of cell growth positive regulation of T cell anergy ubiquitin-dependent protein catabolic process apoptotic process inflammatory response Notch signaling pathway protein ubiquitination negative regulation of NF-kappaB transcription factor activity negative regulation of type I interferon production protein K29-linked ubiquitination protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process innate immune response positive regulation of protein catabolic process negative regulation of JNK cascade negative regulation of alpha-beta T cell proliferation viral entry into host cell negative regulation of defense response to virus defense response to virus nucleotide-binding oligomerization domain containing signaling pathway protein K63-linked ubiquitination protein K48-linked ubiquitination regulation of protein deubiquitination protein polyubiquitination immune system process
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