Invitrogen

ITGA2 Monoclonal Antibody (16B4)

View all (68) CD49b (Integrin alpha 2) antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-80784

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (Frozen) (IHC (F))

30-40 µg/mL
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-

Flow Cytometry (Flow)

30 µg/mL
-

ELISA (ELISA)

10 µg/mL
-

Immunoprecipitation (IP)

25-50 µg/mL
-

Functional Assay (Functional)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

16B4

Immunogen

Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_928516

Product Specific Information

This antibody does not react with rat.

Sodium azide removal by dialysis is recommended prior to use in functional assays. Heat-mediated antigen retrieval using Tris/EDTA (pH 9) is recommended for the staining of paraffin sections.

Mouse anti Human CD49b antibody, clone 16B4 recognizes human CD49b, also known as integrin α2, collagen receptor, Platelet membrane glycoprotein Ia or VLA-2 subunit alpha. CD49b is a 1181 amino acid approximately 160 kDa single pass type-1 transmembrane glycoprotein possessing multiple FG-GAP repeats and a single VWFA domain.

Target Information

CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha 2 subunit of VLA-2 receptor; BDPLT9; CD49 antigen-like family member B; CD49b; Collagen receptor; GPIa; human platelet alloantigen system 5; Integrin alpha-2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); platelet antigen Br; platelet glycoprotein GPIa; Platelet membrane glycoprotein Ia; very late activation protein 2 receptor, alpha-2 subunit; VLA-2 subunit alpha; VLA2 receptor

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Gene Aliases: BR; CD49B; GPIa; HPA-5; ITGA2; VLA-2; VLAA2

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UniProt ID: (Human) P17301

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Entrez Gene ID: (Human) 3673

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Function(s)
virus receptor activity integrin binding protein binding collagen binding protein complex binding collagen receptor activity laminin binding metal ion binding protein heterodimerization activity collagen binding involved in cell-matrix adhesion
Process(es)
response to hypoxia positive regulation of leukocyte migration substrate-dependent cell migration hypotonic response cell adhesion cell-matrix adhesion integrin-mediated signaling pathway female pregnancy blood coagulation cell proliferation organ morphogenesis positive regulation of epithelial cell migration positive regulation of alkaline phosphatase activity response to amine response to muscle activity positive regulation of smooth muscle cell migration extracellular matrix organization mammary gland development positive regulation of cell projection organization cell-substrate adhesion positive regulation of collagen biosynthetic process positive regulation of collagen binding response to L-ascorbic acid cell adhesion mediated by integrin collagen-activated signaling pathway response to drug positive regulation of DNA binding skin morphogenesis establishment of protein localization positive regulation of translation positive regulation of cell adhesion positive regulation of smooth muscle contraction viral entry into host cell focal adhesion assembly mesodermal cell differentiation positive regulation of smooth muscle cell proliferation positive regulation of inflammatory response positive regulation of positive chemotaxis detection of mechanical stimulus involved in sensory perception of pain positive regulation of transmission of nerve impulse positive regulation of phagocytosis, engulfment hepatocyte differentiation response to parathyroid hormone cellular response to mechanical stimulus cellular response to estradiol stimulus
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