Invitrogen

ITGA4 Monoclonal Antibody (TA-2)

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ITGA4 Antibody (MA49D7) in Flow

Flow cytometry analysis of CD49d in THP-1 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD49d monoclonal antibody (Product # MA49D7) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody ... View More

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Product Details

MA49D7

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-dependent

Flow Cytometry (Flow)

Assay-dependent

View 2 publications 2 publications

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Neutralization (Neu)

Assay-dependent

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

TA-2

Immunogen

Rat 150 kD alpha4 chain of VLA-4 (CD49d).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_223594

Product Specific Information

MA49D7 targets CD49d in FACS, IHC, and Neu applications and shows reactivity with Rat and Human samples.

The MA49D7 immunogen is rat 150 kD alpha4 chain of VLA-4 (CD49d).

MA49D7 detects CD49d which has a predicted molecular weight of approximately 111 kDa.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.

Nature neuroscience

Kendirli A,de la Rosa C,Lämmle KF,Eglseer K,Bauer IJ,Kavaka V,Winklmeier S,Zhuo L,Wichmann C,Gerdes LA,Kümpfel T,Dornmair K,Beltrán E,Kerschensteiner M,Kawakami N

MA49D7 was used in Flow Cytometry to reveal key regulators of the fundamental step in the induction of MS lesions.

Sun Oct 01 00:00:00 UTC 2023

Resting CD4+ effector memory T cells are precursors of bystander-activated effectors: a surrogate model of rheumatoid arthritis synovial T-cell function.

Arthritis research & therapy

Brennan FM,Smith NM,Owen S,Li C,Amjadi P,Green P,Andersson A,Palfreeman AC,Hillyer P,Foey A,Beech JT,Feldmann M

MA49D7 was used in blocking/activating experiment to study the role of resting CD4+ effector memory T cells in rheumatoid arthritis

Tue Sep 23 00:00:00 UTC 2008

VCAM-1 has a tissue-specific role in mediating interleukin-4-induced eosinophil accumulation in rat models: evidence for a dissociation between endothelial-cell VCAM-1 expression and a functional role in eosinophil migration.

Blood

Larbi KY,Allen AR,Tam FW,Haskard DO,Lobb RR,Silva PM,Nourshargh S

MA49D7 was used in ELISA to investigate the role of endothelial-cell VCAM-1 in eosinophil accumulation

Wed Nov 15 00:00:00 UTC 2000

VCAM-1 contributes to rapid eosinophil accumulation induced by the chemoattractants PAF and LTB4: evidence for basal expression of functional VCAM-1 in rat skin.

Immunology

Davies D,Larbi K,Allen A,Sanz M,Weg VB,Haskard DO,Lobb RR,Nourshargh S

MA49D7 was used in blocking/activating experiment to study the role of VCAM-1 in eosinophil accumulation

Sat May 01 00:00:00 UTC 1999

Lymphocyte subpopulations in lymphoid organs of rats after acute resistance exercise.

Medicine and science in sports and exercise

Mastro AM,Schlosser DA,Grove DS,Lincoski C,Pishak SA,Gordon S,Kraemer WJ

MA49D7 was used in flow cytometry to investigate the changes of lymphocyte subpopulations in vivo with acute resistance exercise

Fri Jan 01 00:00:00 UTC 1999

Inhibition of in vivo lymphocyte migration to inflammation and homing to lymphoid tissues by the TA-2 monoclonal antibody. A likely role for VLA-4 in vivo.

Journal of immunology (Baltimore, Md. : 1950)

Issekutz TB

MA49D7 was used in blocking/activating experiment to investigate the role of VLA-4 in the migration of lymphocyte under inflammation conditions

Sun Dec 15 00:00:00 UTC 1991

Effect of a new monoclonal antibody, TA-2, that inhibits lymphocyte adherence to cytokine stimulated endothelium in the rat.

Journal of immunology (Baltimore, Md. : 1950)

Issekutz TB,Wykretowicz A

MA49D7 was used in immunoprecipitation to study the lymphocyte and endothelium adhesion in rats

Mon Jul 01 00:00:00 UTC 1991

Bioinformatics

Protein Aliases: 269C wild type; alpha 4 subunit of VLA-4 receptor; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49d; Integrin alpha-4; Integrin alpha-IV; ITGA-IV; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha

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Gene Aliases: CD49D; IA4; ITGA4

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UniProt ID: (Human) P13612

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Entrez Gene ID: (Human) 3676, (Rat) 311144

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Function(s)

fibronectin binding protein binding coreceptor activity C-X3-C chemokine binding metal ion binding cell adhesion molecule binding protein antigen binding

Process(es)

blood vessel remodeling positive regulation of leukocyte migration cell-matrix adhesion involved in ameboidal cell migration cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion integrin-mediated signaling pathway heart development cell migration heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading endodermal cell differentiation receptor clustering leukocyte tethering or rolling diapedesis face development chorio-allantoic fusion cellular response to cytokine stimulus T cell migration negative regulation of protein homodimerization activity import into cell positive regulation of leukocyte cell-cell adhesion positive regulation of leukocyte tethering or rolling positive regulation of T cell migration B cell differentiation extracellular matrix organization regulation of immune response leukocyte migration clathrin-mediated extracellular exosome endocytosis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ITGA4 Monoclonal Antibody (TA-2)

ITGA4 Antibody (MA49D7) in Flow

Product Details

MA49D7

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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