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Bethyl Laboratories
ITGA4/CD49D/Integrin alpha-4 Polyclonal Antibody
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ITGA4/CD49D/Integrin alpha-4 Antibody (A305-255A) in WB
Detection of human and mouse ITGA4 by western blot. Samples: Whole cell lysate from Jurkat (5, 15 and 50 µg), and HeLa (50 µg), HEK293T (50 µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ITGA4 antibody (Product # A305-255A) (lot A305-255A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A305-255A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 982 to 1032 of human Integrin alpha-4.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 269C wild type; alpha 4 subunit of VLA-4 receptor; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49d; Integrin alpha-4; Integrin alpha-IV; integrin alpha4B; ITGA-IV; LPAM subunit alpha; Lymphocyte Peyer patch adhesion molecules subunit alpha; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha
Gene Aliases: CD49D; IA4; ITGA4; Itga4B
UniProt ID: (Human) P13612, (Mouse) Q00651
Entrez Gene ID: (Human) 3676, (Mouse) 16401
blood vessel remodeling
positive regulation of leukocyte migration
cell-matrix adhesion involved in ameboidal cell migration
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
heart development
single organismal cell-cell adhesion
cell migration
heterotypic cell-cell adhesion
substrate adhesion-dependent cell spreading
receptor clustering
leukocyte tethering or rolling
diapedesis
face development
mesenchyme development
chorio-allantoic fusion
visceral serous pericardium development
T cell migration
negative regulation of protein homodimerization activity
import into cell
positive regulation of leukocyte cell-cell adhesion
positive regulation of leukocyte tethering or rolling
positive regulation of T cell migration
B cell differentiation
extracellular matrix organization
endodermal cell differentiation
regulation of immune response
leukocyte migration
cellular response to cytokine stimulus
clathrin-mediated extracellular exosome endocytosis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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