Invitrogen

ITGA5 Polyclonal Antibody

ITGA5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 6

ITGA5 Antibody (PA5-82027) in IHC (P)

Immunohistochemical analysis of ITGA5 in human endometrium using ITGA5 Polyclonal Antibody (Product # PA5-82027) shows weak to moderate membranous positivity in smooth muscle cells.

Product Image Gallery

ITGA5 Antibody — Relative expression in western blot: Antibody specificity was demonstrated by detection of known differential basal expression of the target across tissue/cell models. Expression of ITGA5 was observed specifically in U2OS cells and in SK-MEL-30 cells using anti-ITGA5 Polyclonal Antibody (Product #PA5-82027) in western blot. The relative expression levels of ITGA5 within each cell line is shown using RNA-Seq. {RE}

Product Details

PA5-82027

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human ITGA5. Recombinant protein control fragment (Product #RP-102172).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2789188

Product Specific Information

Immunogen sequence: DQPQKEEDLG PAVHHVYELI NQGPSSISQG VLELSCPQAL EGQQLLYVTR VTGLNCTTNH PINPKGLELD PEGSLHHQQK REAPSRSSAS SGPQILKCPE AECFRLRCEL GPLHQQESQS LQLHFRVWA

Target Information

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Osteocytes regulate bone anabolic response to mechanical loading in male mice via activation of integrin α5.

Bone research

Zhao D,Hua R,Riquelme MA,Cheng H,Guda T,Xu H,Gu S,Jiang JX

PA5-82027 was used in Western Blotting to show an indispensable role of integrin α5 in osteocytes in the anabolic action of mechanical loading on skeletal tissue through activation of hemichannels and PGE2-evoked gene expression.

Mon Jul 18 00:00:00 UTC 2022

Translation of Tudor-SN, a novel terminal oligo-pyrimidine (TOP) mRNA, is regulated by the mTORC1 pathway in cardiomyocytes.

RNA biology

Gan S,Su C,Ma J,Liu M,Cui X,Xin L,Ren Y,Gao X,Ge L,Wei M,Yang J

PA5-82027 was used in Western Blot to suggest that the translational efficiency of Tudor-SN is regulated by the mTORC1 pathway in myocardia and that Tudor-SN is involved in mTORC1-mediated regulation of cardiomyocytic proliferation and cardiac development.

Tue Jun 01 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: CD49 antigen-like family member E; CD49e; fibronectin receptor alpha polypeptide; Fibronectin receptor subunit alpha; fibronectin receptor, alpha polypeptide; fibronectin receptor, alpha subunit; integrin alpha 5; Integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); very late activation protein 5, alpha subunit; VLA-5

View more View less

Gene Aliases: CD49e; FNRA; ITGA5; VLA-5; VLA5; VLA5A

View more View less

UniProt ID: (Human) P08648, (Mouse) P11688

View more View less

Entrez Gene ID: (Human) 3678, (Mouse) 16402

View more View less

Function(s)

virus receptor activity epidermal growth factor receptor binding platelet-derived growth factor receptor binding integrin binding protein binding vascular endothelial growth factor receptor 2 binding metal ion binding cell adhesion molecule binding

Process(es)

cell-substrate junction assembly cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion integrin-mediated signaling pathway memory positive regulation of cell-substrate adhesion positive regulation of cell migration cell-substrate adhesion cell-cell adhesion mediated by integrin heterotypic cell-cell adhesion endodermal cell differentiation positive regulation of peptidyl-tyrosine phosphorylation negative regulation of anoikis angiogenesis extracellular matrix organization positive regulation of vascular endothelial growth factor receptor signaling pathway wound healing, spreading of epidermal cells viral entry into host cell leukocyte migration positive regulation of sprouting angiogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

ITGA5 Polyclonal Antibody

ITGA5 Antibody (PA5-82027) in IHC (P)

Product Details

PA5-82027

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!