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Invitrogen
ITGA5 Recombinant Rabbit Monoclonal Antibody (12H40L20)
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ITGA5 Antibody (702178) in WB
Western blot analysis was performed on Membrane enriched extracts (30 µg lysate) of U-87 MG (Lane 1), U-937 (Lane 2), THP-1 (Lane 3) and HeLa (Lane 4). The blots were probed with Anti-Integrin alpha 5 beta 1 Recombinant Rabbit Monoclonal Antibody (Product # 702178, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:4000 dilution). A ~120 kDa band corresponding to Integrin alpha 5 beta 1 was observed across the cell lines tested... View More
Product Details
702178
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
12H40L20
Immunogen
Protein corresponding to human Integrin alpha5 beta1 (aa800-aa1049)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2716880
Product Specific Information
This antibody is predicted to react with Monkey, Horse, Cat, Bovine
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD49 antigen-like family member E; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha polypeptide; fibronectin receptor, alpha subunit; integrin alpha 5; Integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); very late activation protein 5, alpha subunit; VLA-5
Gene Aliases: CD49e; FNRA; ITGA5; VLA-5; VLA5A
UniProt ID: (Human) P08648
Entrez Gene ID: (Human) 3678
angiogenesis
cell-substrate junction assembly
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
integrin-mediated signaling pathway
memory
positive regulation of cell-substrate adhesion
extracellular matrix organization
positive regulation of cell migration
positive regulation of vascular endothelial growth factor receptor signaling pathway
cell-substrate adhesion
cell-cell adhesion mediated by integrin
heterotypic cell-cell adhesion
wound healing, spreading of epidermal cells
endodermal cell differentiation
viral entry into host cell
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte migration
positive regulation of sprouting angiogenesis
negative regulation of anoikis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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