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Product Details
PA5-114752
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ITGB1BP1(Accession O14713), corresponding to amino acid residues L137-L187.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2899388
Product Specific Information
Antibody detects endogenous levels of total ITBP1.
Target Information
Key regulator of the integrin-mediated cell-matrix interaction signaling by binding to the ITGB1 cytoplasmic tail and preventing the activation of integrin alpha-5/beta-1 (heterodimer of ITGA5 and ITGB1) by talin or FERMT1. Plays a role in cell proliferation, differentiation, spreading, adhesion and migration in the context of mineralization and bone development and angiogenesis. Stimulates cellular proliferation in a fibronectin-dependent manner. Involved in the regulation of beta-1 integrin-containing focal adhesion (FA) site dynamics by controlling its assembly rate during cell adhesion; inhibits beta-1 integrin clustering within FA by directly competing with talin TLN1, and hence stimulates osteoblast spreading and migration in a fibronectin-and/or collagen-dependent manner. Acts as a guanine nucleotide dissociation inhibitor (GDI) by regulating Rho family GTPases during integrin-mediated cell matrix adhesion; reduces the level of active GTP-bound form of both CDC42 and RAC1 GTPases upon cell adhesion to fibronectin. Stimulates the release of active CDC42 from the membranes to maintain it in an inactive cytoplasmic pool. Participates in the translocation of the Rho-associated protein kinase ROCK1 to membrane ruffles at cell leading edges of the cell membrane, leading to an increase of myoblast cell migration on laminin. Plays a role in bone mineralization at a late stage of osteoblast differentiation; modulates the dynamic formation of focal adhesions into fibrillar adhesions, which are adhesive structures responsible for fibronectin deposition and fibrillogenesis. Plays a role in blood vessel development; acts as a negative regulator of angiogenesis by attenuating endothelial cell proliferation and migration, lumen formation and sprouting angiogenesis by promoting AKT phosphorylation and inhibiting ERK1/2 phosphorylation through activation of the Notch signaling pathway. Promotes transcriptional activity of the MYC promoter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bodenin; DKFZp686K08158; ICAP-1; integrin beta 1 binding protein 1; Integrin beta-1-binding protein 1; Integrin cytoplasmic domain-associated protein 1; integrin cytoplasmic domain-associated protein 1-alpha; integrin cytoplasmic domain-associated protein 1-beta
Gene Aliases: AI449260; AU019480; ICAP-1A; ICAP-1alpha; ICAP-1B; ICAP1; ICAP1A; ICAP1B; ITGB1BP1
UniProt ID: (Human) O14713, (Mouse) O35671
Entrez Gene ID: (Human) 9270, (Mouse) 16413
blood vessel endothelial cell proliferation involved in sprouting angiogenesis
transcription, DNA-templated
negative regulation of protein kinase activity
negative regulation of cell adhesion involved in substrate-bound cell migration
cell-matrix adhesion
Notch signaling pathway
integrin-mediated signaling pathway
positive regulation of cell proliferation
negative regulation of cell proliferation
positive regulation of endothelial cell migration
negative regulation of fibroblast migration
protein transport
cell migration
cell differentiation
biomineral tissue development
negative regulation of protein binding
activation of protein kinase B activity
integrin activation
regulation of cell adhesion mediated by integrin
tube formation
intracellular signal transduction
cellular response to vascular endothelial growth factor stimulus
regulation of GTPase activity
receptor clustering
cellular response to fibroblast growth factor stimulus
positive regulation of Notch signaling pathway
positive regulation of transcription from RNA polymerase II promoter
regulation of blood vessel size
myoblast migration
positive regulation of stress fiber assembly
positive regulation of cell division
positive regulation of focal adhesion assembly
negative regulation of focal adhesion assembly
positive regulation of protein kinase B signaling
negative regulation of ERK1 and ERK2 cascade
negative regulation of cell migration involved in sprouting angiogenesis
positive regulation of protein targeting to membrane
negative regulation of protein targeting to membrane
negative regulation of substrate adhesion-dependent cell spreading
regulation of integrin-mediated signaling pathway
angiogenesis
regulation of transcription, DNA-templated
cell adhesion
regulation of cell adhesion
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