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Invitrogen
IkB zeta Monoclonal Antibody (hft2nap), PE, eBioscience™
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FIGURE: 1 / 1
IkB zeta Antibody (12-9853-42) in Flow
Normal human peripheral blood cells were unstimulated (left) or stimulated with recombinant human IL-2 (Product # 14-8029-81), IL-12 (Product # 14-8129-62), and IL-18 (right) for 6 hours. Cultures were then harvested, surface-stained with Anti-Human CD56 (NCAM) APC (Product # 17-0567-42), and fixed and permeabilized with the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). After fixation and permeabilization, cells were stained with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) or Anti-Human I kappa B Zeta PE... View More
Product Details
12-9853-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
hft2nap
Immunogen
Human IkBz N-terminal Fragment (1-200)
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572743
Product Specific Information
Description: The hft2nap monoclonal antibody reacts with human IkB zeta (INAP, MAIL, IkBz). IkBz is a member of the IkB family of cytoplasmic proteins and transcription factors and also shares structural homology with Bcl-3. It is induced in monocytes, macrophages and B cells in response to TLR and IL-1R signaling. In NK cells, IkBz is rapidly upregulated in response to IL-12 and IL-18 and is necessary for production of IFN gamma. In T cells, IkBz is not necessary for IFN gamma and IL-4 production by Th1 and Th2 cells, respectively. However in Th17 cells, IkBz acts in concert with ROR alpha and ROR gamma t to upregulate IL-17A, IL-17F, IL-21, IL-22 and IL-23R expression.
Applications Reported: This hft2nap antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This hft2nap antibody has been pre-titrated and tested on stimulated human peripheral blood mononuclear cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
I-kappa-B-zeta (also known as MAIL and INAP) is an ankyrin-repeat-containing nuclear protein that is highly homologous to the IkappaB family member Bcl-3. Transcription of IkappaBzeta is upregulated by stimulation with TLR ligands, IL-1 and IL-6 in cultured B-lymphocytes and monocytes/macrophages, but only faintly so in T-lymphocytes, fibroblasts, and endothelial cells. IkappaB-zeta preferentially associates with the NF-kappaB subunit p50 rather than p65 and recombinant IkappaB-zeta proteins inhibit the DNA binding of the p65/p50 heterodimer and the p50/p50 homodimer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ30225; FLJ34463; I-kappa-B-zeta; Ikappa B-zeta variant 3; IkappaB-zeta; ikappaBzeta; IkB-zeta; IL-1 inducible nuclear ankyrin-repeat protein; INAP; MAIL; Molecule possessing ankyrin repeats induced by lipopolysaccharide; NF-kappa-B inhibitor zeta; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta; OTTHUMP00000214340
Gene Aliases: IKBZ; INAP; MAIL; NFKBIZ
UniProt ID: (Human) Q9BYH8
Entrez Gene ID: (Human) 64332
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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