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ELTI
Indole-3-butyric acid Polyclonal Antibody
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Product Details
T0158P
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Antibody
Immunogen
Indole-3-butyric acid
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
PEG precipitation
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4°C or -20°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Cross Reactivity, Indole-3-aceteic acid < 0.1 %.
The antibody may be used in ELISA and Immuno-affinity-chromatography (IAC). Samples may include cutting medium samples and extraction of these samples may be performed using PBS-10% methanol, followed by neutralisation of the extract. Suggested dilution 1:12,800 in indirect ELISA (this titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). Plates are coated with 400 ng/mL ovalbumine-conjugated indole-3-butyric acid. HRP-conjugated anti-chicken IgY should be used as a tracer in a 1:8,000 dilution.
Store at 4 °C or -20°C. The working antibody solution is stable for at least 7 days at 4°C. Precautions should be taken for storage for longer periods. Problems of long term stability may occur with highly diluted solutions. No other preservative agent has been added to the present formulation. For long storage purposes in solution the addition of sodium azide to 0.02 % is advised with the appropriate precautions of use.
Target Information
Indole-3-butyric acid (IBA) is a plant hormone belonging to the group of auxins. It is used to stimulate root formation in plant cuttings.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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