Invitrogen

Insulin Monoclonal Antibody (ICBTACLS), eBioscience™

3 Published Figures
8 References
View all (73) Insulin antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

14-9769-82

Applications
Tested Dilution
Publications

Western Blot (WB)

5 µg/mL
- -

Immunohistochemistry (IHC)

-
View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

0.25 µg/mL
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

ICBTACLS

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573014

Product Specific Information

Description: This ICBTACLS monoclonal antibody reacts with the hormone insulin, responsible for regulation of carbohydrate and fat metabolism. Blood glucose levels are maintained by insulin. Insulin secretion from pancreatic beta-cells within the islets of Langerhans results in uptake of excess glucose from the blood into organs and its storage in the form of glycogen. Patients with type 1 diabetes have an autoimmune destruction of insulin-producing beta-cells in the pancreas, resulting in insulin deficiency and elevated levels of blood glucose. Patients with type 2 diabetes have insulin resistance where insulin becomes less effective at reducing the amount of blood glucose. Insulin is also important for proper endothelial cell function in with respect to vascular elasticity in response to changing blood pressure and plays a role in cognition, learning, and memory.

The ICBTACLS antibody recognizes human, mouse, and rat insulin.

Applications Reported: This ICBTACLS antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.

Applications Tested: This ICBTACLS antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval and can be used at less than or equal to 0.25 µg/mL. This ICBTACLS antibody has been tested by western blot of non-reduced pancreatic tissue lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Ins; Insulin; Insulin-1; Insulin-2; preproinsulin; proinsulin

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Gene Aliases: AA986540; IDDM; IDDM1; IDDM2; ILPR; INS; Ins-1; Ins-2; Ins1; Ins2; Ins2-rs1; InsII; IRDN; Mody; MODY10; Mody4; proinsulin

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UniProt ID: (Human) P01308, (Rat) P01322, (Mouse) P01325, (Rat) P01323, (Mouse) P01326

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Entrez Gene ID: (Human) 3630, (Rat) 24505, (Mouse) 16333, (Rat) 24506, (Mouse) 16334

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Function(s)
protease binding insulin receptor binding insulin-like growth factor receptor binding hormone activity protein binding identical protein binding chaperone binding protein complex binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter activation of MAPK activity glucose metabolic process ER overload response myoblast fusion biological_process insulin receptor signaling pathway lipid catabolic process regulation of protein binding positive regulation of phosphatidylinositol 3-kinase activity positive regulation of peptidyl-tyrosine phosphorylation canonical Wnt signaling pathway intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress MAPK cascade negative regulation of acute inflammatory response regulation of cellular amino acid metabolic process acute-phase response G-protein coupled receptor signaling pathway positive regulation of cell proliferation response to organic substance positive regulation of phosphatidylinositol 3-kinase signaling glucose transport regulation of transmembrane transporter activity activation of protein kinase B activity positive regulation of cellular protein metabolic process negative regulation of protein oligomerization regulation of protein localization negative regulation of NAD(P)H oxidase activity response to cytokine wound healing negative regulation of protein catabolic process glucose homeostasis positive regulation of MAPK cascade response to peptide hormone positive regulation of glycogen biosynthetic process positive regulation of DNA replication negative regulation of glycogen catabolic process positive regulation of glycolytic process positive regulation of mitotic nuclear division negative regulation of proteolysis negative regulation of fatty acid metabolic process positive regulation of glucose import positive regulation of insulin receptor signaling pathway alpha-beta T cell activation regulation of protein secretion negative regulation of protein secretion positive regulation of cytokine secretion negative regulation of lipid catabolic process positive regulation of NF-kappaB transcription factor activity response to cAMP positive regulation of protein kinase B signaling fatty acid homeostasis negative regulation of respiratory burst involved in inflammatory response positive regulation of respiratory burst positive regulation of protein localization to nucleus negative regulation of feeding behavior regulation of transcription, DNA-templated ER to Golgi vesicle-mediated transport cell-cell signaling positive regulation of gene expression positive regulation of cell growth positive regulation of cell migration positive regulation of protein autophosphorylation cellular protein metabolic process positive regulation of nitric oxide biosynthetic process positive regulation of cell differentiation negative regulation of gluconeogenesis negative regulation of vasodilation positive regulation of vasodilation positive regulation of lipid biosynthetic process positive regulation of nitric-oxide synthase activity positive regulation of peptide hormone secretion positive regulation of brown fat cell differentiation negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway carbohydrate metabolic process activation of JUN kinase activity regulation of gene expression myotube differentiation regulation of phosphorylation
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