Invitrogen

Integrin alpha 4 beta 7 (LPAM-1) Monoclonal Antibody (DATK32 (DATK-32)), PE, eBioscience™

1 Published Figure

9 References

View all (13) LPAM-1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

Integrin alpha 4 beta 7 (LPAM-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

Integrin alpha 4 beta 7 (LPAM-1) Antibody (12-5887-82) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.5 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.5 µg of Anti-Mouse Integrin alpha 4 beta 7 (LPAM-1) PE (right). Cells in the small scatter population were used for analysis.

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Product Details

12-5887-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DATK32 (DATK-32)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_657803

Product Specific Information

Description: The DATK32 monoclonal antibody binds to a combinatorial epitope of mouse LPAM-1 (alpha 4/ beta 7), which is a heterodimer of the the 154 kDa alpha 4 subunit and the 130 kDa beta 7 subunit. In mouse, the beta 7 subunit is found on the majority of mature lymphocytes and a small population of thymocytes and bone marrow cells. LPAM-1 is an integrin involved in the transendothelial migration of lymphocytes across high endothelial venules (HEV), and is know to bind several ligands including MAdCAM-1, VCAM-1 and fibronectin. Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block LPAM-1-mediated cell adhesion.

Applications Reported: This DATK32 (DATK-32) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DATK32 (DATK-32) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Integrin beta 7 associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Ligands for LPAM-1 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the ligand of HML-1 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Krüppel-like factor 2 controls IgA plasma cell compartmentalization and IgA responses.

Mucosal immunology

Wittner J,Schulz SR,Steinmetz TD,Berges J,Hauke M,Channell WM,Cunningham AF,Hauser AE,Hutloff A,Mielenz D,Jäck HM,Schuh W

Published figure using Integrin alpha 4 beta 7 (LPAM-1) monoclonal antibody (Product # 12-5887-82) in Flow Cytometry

Fri Apr 01 00:00:00 UTC 2022

Vitamin A Impairs the Reprogramming of Tregs into IL-17-Producing Cells during Intestinal Inflammation.

BioMed research international

Tejón G,Manríquez V,De Calisto J,Flores-Santibáñez F,Hidalgo Y,Crisóstomo N,Fernández D,Sauma D,Mora JR,Bono MR,Rosemblatt M

12-5887 was used in Flow cytometry/Cell sorting to study the contribution of different factors to the reprogramming of iTregs into IL-17-producing cells, showing that vitamin A impairs reprogramming during intestinal inflammation.

Fri Sep 02 00:00:00 UTC 2016

Identification and characterization of latency-associated peptide-expressing γδ T cells.

Nature communications

Rezende RM,da Cunha AP,Kuhn C,Rubino S,M'Hamdi H,Gabriely G,Vandeventer T,Liu S,Cialic R,Pinheiro-Rosa N,Oliveira RP,Gaublomme JT,Obholzer N,Kozubek J,Pochet N,Faria AMC,Weiner HL

12-5887 was used in Flow cytometry/Cell sorting to describe a subset of regulatory γδ T cells that express the latency-associated peptide, a membrane-bound TGF-β1.

Tue Dec 08 00:00:00 UTC 2015

Self-recognition of the endothelium enables regulatory T-cell trafficking and defines the kinetics of immune regulation.

Nature communications

Fu H,Kishore M,Gittens B,Wang G,Coe D,Komarowska I,Infante E,Ridley AJ,Cooper D,Perretti M,Marelli-Berg FM

12-5887 was used in Flow cytometry/Cell sorting to explain the mechanisms underlying the powerful migratory capacity of Treg cells and their ability to maintain optimal T cell ratios.

Fri Mar 14 00:00:00 UTC 2014

A critical role for the retinoic acid signaling pathway in the pathophysiology of gastrointestinal graft-versus-host disease.

Blood

Chen X,Dodge J,Komorowski R,Drobyski WR

12-5887 was used in Flow cytometry/Cell sorting to demonstrate that the administration of exogenous retinoic acid augments the accumulation of proinflammatory T cells.

Thu May 09 00:00:00 UTC 2013

Notch, Id2, and RORγt sequentially orchestrate the fetal development of lymphoid tissue inducer cells.

The Journal of experimental medicine

Cherrier M,Sawa S,Eberl G

12-5887 was used in Flow cytometry/Cell sorting to investigate the development of lymphoid tissue inducer (LTi) cells from common lymphoid progenitors (CLPs) in the fetal liver, showing that sequential changes in expression of Notch, Id2, and RORγt orchestrate the development of LTi cells.

Mon Apr 09 00:00:00 UTC 2012

Vitamin A deficiency impairs vaccine-elicited gastrointestinal immunity.

Journal of immunology (Baltimore, Md. : 1950)

Kaufman DR,De Calisto J,Simmons NL,Cruz AN,Villablanca EJ,Mora JR,Barouch DH

12-5887 was used in Flow cytometry/Cell sorting to suggest that oral vitamin A supplementation may be important for optimizing the success of vaccines against HIV-1 and other mucosal pathogens in the developing world.

Mon Aug 15 00:00:00 UTC 2011

Control of alpha4beta7 integrin expression and CD4 T cell homing by the beta1 integrin subunit.

Journal of immunology (Baltimore, Md. : 1950)

DeNucci CC,Pagán AJ,Mitchell JS,Shimizu Y

12-5887 was used in Flow cytometry/Cell sorting to investigate the role of the beta 1 subunit of integrin on CD4 T cell homing.

Mon Mar 01 00:00:00 UTC 2010

Efficient induction of CCR9 on T cells requires coactivation of retinoic acid receptors and retinoid X receptors (RXRs): exaggerated T Cell homing to the intestine by RXR activation with organotins.

Journal of immunology (Baltimore, Md. : 1950)

Takeuchi H,Yokota A,Ohoka Y,Kagechika H,Kato C,Song SY,Iwata M

12-5887 was used in Flow cytometry/Cell sorting to study the effect of RXR activation on the RAR-dependent CCR9 expression on T cells, and their homing capacity to the small intestine.

Mon Nov 01 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: CD49 antigen-like family member D; CD49d; Integrin alpha-4; Integrin alpha-IV; integrin alpha4B; Integrin beta-7; Integrin beta-P; LPAM subunit alpha; Lymphocyte Peyer patch adhesion molecules subunit alpha; M290 IEL antigen; VLA; VLA 4; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha; VLA4 subunit alpha

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Gene Aliases: CD49D; Itga4; Itga4B; Itgb7; Ly69

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UniProt ID: (Mouse) Q00651, (Mouse) P26011

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Entrez Gene ID: (Mouse) 16401, (Mouse) 16421

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Function(s)

fibronectin binding metal ion binding cell adhesion molecule binding protein antigen binding receptor activity

Process(es)

cell-matrix adhesion involved in ameboidal cell migration cell adhesion cell-matrix adhesion integrin-mediated signaling pathway heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading receptor clustering leukocyte migration leukocyte tethering or rolling T cell migration blood vessel remodeling positive regulation of leukocyte migration heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion heart development single organismal cell-cell adhesion cell migration diapedesis face development mesenchyme development chorio-allantoic fusion visceral serous pericardium development negative regulation of protein homodimerization activity import into cell positive regulation of leukocyte cell-cell adhesion positive regulation of leukocyte tethering or rolling positive regulation of T cell migration

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Integrin alpha 4 beta 7 (LPAM-1) Monoclonal Antibody (DATK32 (DATK-32)), PE, eBioscience™

Integrin alpha 4 beta 7 (LPAM-1) Antibody (12-5887-82) in Flow

Product Details

12-5887-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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