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Integrin alpha-5 Polyclonal Antibody

View all (33) CD49e (Integrin alpha 5) antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (7)

Integrin alpha-5 Antibody in Western Blot (WB)

Integrin alpha-5 Antibody in Western Blot (WB)

FIGURE: 1 / 7

Integrin alpha-5 Antibody (10569-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 10569-1-AP) Integrin Alpha-5 antibody at dilution of 1:3,000 incubated at room temperature for 1.5 hours.

Integrin alpha-5 Antibody in Western Blot (WB)

Integrin alpha-5 Antibody in Western Blot (WB)

Integrin alpha-5 Antibody in Western Blot (WB)

Integrin alpha-5 Antibody in Western Blot (WB)

Integrin alpha-5 Antibody in Western Blot (WB)

Integrin alpha-5 Antibody in Western Blot (WB)

Integrin alpha-5 Antibody in Western Blot (WB)

Product Image Gallery

Integrin alpha-5 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 10569-1-AP) Integrin Alpha-5 antibody at dilution of 1:3,000 incubated at room temperature for 1.5 hours.

Integrin alpha-5 Antibody in Western Blot (WB) — WB result of Integrin alpha-5 antibody (Product # 10569-1-AP; 1:2,000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin alpha-5 transfected HeLa cells.

Integrin alpha-5 Antibody in Western Blot (WB) — Human placenta tissue were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Integrin alpha-5 Antibody in Western Blot (WB) — 3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Integrin alpha-5 Antibody in Western Blot (WB) — COS-7 cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Integrin alpha-5 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Integrin alpha-5 Antibody in Western Blot (WB) — A549 cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Product Details

10569-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 17 publications 17 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 6 publications 6 publications

Miscellaneous PubMed (MISC)

-

View 2 publications 2 publications

Not applicable (N/A)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Integrin alpha-5 Fusion Protein Ag0860 (749-1049 aa encoded by BC008786)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.28 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: FTVPHLRDT KKTIQFDFQI LSKNLNNSQS DVVSFRLSVE AQAQVTLNGV SKPEAVLFPV SDWHPRDQPQ KEEDLGPAVH HVYELINQGP SSISQGVLEL SCPQALEGQQ LLYVTRVTGL NCTTNHPINP KGLELDPEGS LHHQQKREAP SRSSASSGPQ ILKCPEAECF RLRCELGPLH QQESQSLQLH FRVWAKTFLQ REHQPFSLQC EAVYKALKMP YRILPRQLPQ KERQVATAVQ WTKAEGSYGV PLWIIILAIL FGLLLLGLLI YILYKLGFFK RSLPYGTAME KAQLKPPATS DA (749-1049 aa encoded by BC008786)

Target Information

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ITGA5 and ITGB1 contribute to Sorafenib resistance by promoting vasculogenic mimicry formation in hepatocellular carcinoma.

Cancer medicine

Shi Y,Shang J,Li Y,Zhong D,Zhang Z,Yang Q,Lai C,Feng T,Yao Y,Huang X

Wed Feb 01 00:00:00 UTC 2023

ITGA5 Promotes Tumor Progression through the Activation of the FAK/AKT Signaling Pathway in Human Gastric Cancer.

Oxidative medicine and cellular longevity

Wang JF,Chen YY,Zhang SW,Zhao K,Qiu Y,Wang Y,Wang JC,Yu Z,Li BP,Wang Z,Chen JQ

Wed Oct 05 00:00:00 UTC 2022

ITGA5 Promotes Tumor Progression through the Activation of the FAK/AKT Signaling Pathway in Human Gastric Cancer.

Oxidative medicine and cellular longevity

Wang JF,Chen YY,Zhang SW,Zhao K,Qiu Y,Wang Y,Wang JC,Yu Z,Li BP,Wang Z,Chen JQ

Wed Oct 05 00:00:00 UTC 2022

ITGA5 Promotes Tumor Progression through the Activation of the FAK/AKT Signaling Pathway in Human Gastric Cancer.

Oxidative medicine and cellular longevity

Wang JF,Chen YY,Zhang SW,Zhao K,Qiu Y,Wang Y,Wang JC,Yu Z,Li BP,Wang Z,Chen JQ

Wed Oct 05 00:00:00 UTC 2022

ITGA5 Promotes Tumor Progression through the Activation of the FAK/AKT Signaling Pathway in Human Gastric Cancer.

Oxidative medicine and cellular longevity

Wang JF,Chen YY,Zhang SW,Zhao K,Qiu Y,Wang Y,Wang JC,Yu Z,Li BP,Wang Z,Chen JQ

Wed Oct 05 00:00:00 UTC 2022

ITGA5 Promotes Tumor Progression through the Activation of the FAK/AKT Signaling Pathway in Human Gastric Cancer.

Oxidative medicine and cellular longevity

Wang JF,Chen YY,Zhang SW,Zhao K,Qiu Y,Wang Y,Wang JC,Yu Z,Li BP,Wang Z,Chen JQ

Wed Oct 05 00:00:00 UTC 2022

ITGA5 Promotes Tumor Progression through the Activation of the FAK/AKT Signaling Pathway in Human Gastric Cancer.

Oxidative medicine and cellular longevity

Wang JF,Chen YY,Zhang SW,Zhao K,Qiu Y,Wang Y,Wang JC,Yu Z,Li BP,Wang Z,Chen JQ

Wed Oct 05 00:00:00 UTC 2022

Angiogenic factor AGGF1 blocks neointimal formation after vascular injury via interaction with integrin α7 on vascular smooth muscle cells.

The Journal of biological chemistry

Yu Y,Li Y,Peng H,Song Q,Da X,Li H,He Z,Ren X,Xu C,Yao Y,Wang QK

Fri Apr 01 00:00:00 UTC 2022

ITGA5 Is a Novel Oncogenic Biomarker and Correlates With Tumor Immune Microenvironment in Gliomas.

Frontiers in oncology

Li S,Zhang N,Liu S,Zhang H,Liu J,Qi Y,Zhang Q,Li X

Tue Apr 05 00:00:00 UTC 2022

Angiopoietin 2 stimulates trophoblast invasion via a mechanism associated with JNK signaling.

Molecular human reproduction

Hou H,Ning F,Zhang JY,Lu Q,Zhang M,Wu P,Chen M,Lash GE

Sat Feb 27 00:00:00 UTC 2021

Role of vasodilator-stimulated phosphoprotein in RANKL-differentiated murine macrophage RAW264.7 cells: Modulation of NF-κB, c-Fos and NFATc1 transcription factors.

Experimental and therapeutic medicine

Hu H,Li C,Zhang H,Wu G,Huang Y

Sat May 01 00:00:00 UTC 2021

Mesenchymal Stem Cells With Cancer-Associated Fibroblast-Like Phenotype Stimulate SDF-1/CXCR4 Axis to Enhance the Growth and Invasion of B-Cell Acute Lymphoblastic Leukemia Cells Through Cell-to-Cell Communication.

Frontiers in cell and developmental biology

Pan C,Fang Q,Liu P,Ma D,Cao S,Zhang L,Chen Q,Hu T,Wang J

Fri Nov 05 00:00:00 UTC 2021

Mesenchymal Stem Cells With Cancer-Associated Fibroblast-Like Phenotype Stimulate SDF-1/CXCR4 Axis to Enhance the Growth and Invasion of B-Cell Acute Lymphoblastic Leukemia Cells Through Cell-to-Cell Communication.

Frontiers in cell and developmental biology

Pan C,Fang Q,Liu P,Ma D,Cao S,Zhang L,Chen Q,Hu T,Wang J

Fri Nov 05 00:00:00 UTC 2021

The cell surface hyaluronidase TMEM2 regulates cell adhesion and migration via degradation of hyaluronan at focal adhesion sites.

The Journal of biological chemistry

Irie F,Tobisawa Y,Murao A,Yamamoto H,Ohyama C,Yamaguchi Y

Fri Aug 27 00:00:00 UTC 2021

Ursolic Acid Regulates Intestinal Microbiota and Inflammatory Cell Infiltration to Prevent Ulcerative Colitis.

Journal of immunology research

Sheng Q,Li F,Chen G,Li J,Li J,Wang Y,Lu Y,Li Q,Li M,Chai K

Mon Nov 29 00:00:00 UTC 2021

Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes.

iScience

Kazan JM,Desrochers G,Martin CE,Jeong H,Kharitidi D,Apaja PM,Roldan A,St Denis N,Gingras AC,Lukacs GL,Pause A

Fri Nov 19 00:00:00 UTC 2021

ITGA5 Predicts Dual-Drug Resistance to Temozolomide and Bevacizumab in Glioma.

Frontiers in oncology

Shi Y,Wu M,Liu Y,Hu L,Wu H,Xie L,Liu Z,Wu A,Chen L,Xu C

Wed Nov 08 00:00:00 UTC 2023

Identification of rare variants in cardiac sodium channel β4-subunit gene SCN4B associated with ventricular tachycardia.

Molecular genetics and genomics : MGG

Yang Q,Xiong H,Xu C,Huang Y,Tu X,Wu G,Fu F,Wang Z,Wang L,Zhao Y,Li S,Huang Y,Wang C,Wang D,Yao Y,Wang F,Wang Y,Xue Y,Wang P,Chen Q,Pu J,Wang QK

Thu Aug 01 00:00:00 UTC 2019

Osteogenesis potential of different titania nanotubes in oxidative stress microenvironment.

Biomaterials

Yu Y,Shen X,Luo Z,Hu Y,Li M,Ma P,Ran Q,Dai L,He Y,Cai K

Fri Jun 01 00:00:00 UTC 2018

Osteogenesis potential of different titania nanotubes in oxidative stress microenvironment.

Biomaterials

Yu Y,Shen X,Luo Z,Hu Y,Li M,Ma P,Ran Q,Dai L,He Y,Cai K

Fri Jun 01 00:00:00 UTC 2018

Quantitative Proteomics Implicates Rictor/mTORC2 in Cell Adhesion.

Journal of proteome research

Wang H,Shao X,He Q,Wang C,Xia L,Yue D,Qin G,Jia C,Chen R

Fri Oct 05 00:00:00 UTC 2018

Quantitative Proteomics Implicates Rictor/mTORC2 in Cell Adhesion.

Journal of proteome research

Wang H,Shao X,He Q,Wang C,Xia L,Yue D,Qin G,Jia C,Chen R

Fri Oct 05 00:00:00 UTC 2018

Dual Receptor Targeting Cell Penetrating Peptide Modified Liposome for Glioma and Breast Cancer Postoperative Recurrence Therapy.

Pharmaceutical research

Qiu Y,Yu Q,Liu Y,Tang J,Wang X,Lu Z,Xu Z,He Q

Thu Apr 26 00:00:00 UTC 2018

Silencing Formin-like 2 inhibits growth and metastasis of gastric cancer cells through suppressing internalization of integrins.

Cancer cell international

Zhong B,Wang K,Xu H,Kong F

Thu Mar 17 00:00:00 UTC 2022

Silencing Formin-like 2 inhibits growth and metastasis of gastric cancer cells through suppressing internalization of integrins.

Cancer cell international

Zhong B,Wang K,Xu H,Kong F

Thu Mar 17 00:00:00 UTC 2022

Dioscin, a potent ITGA5 inhibitor, reduces the synthesis of collagen against liver fibrosis: Insights from SILAC-based proteomics analysis.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Xu L,Yin L,Tao X,Qi Y,Han X,Xu Y,Song S,Li L,Sun P,Peng J

Fri Sep 01 00:00:00 UTC 2017

Dioscin, a potent ITGA5 inhibitor, reduces the synthesis of collagen against liver fibrosis: Insights from SILAC-based proteomics analysis.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Xu L,Yin L,Tao X,Qi Y,Han X,Xu Y,Song S,Li L,Sun P,Peng J

Fri Sep 01 00:00:00 UTC 2017

Evidence of novel miR-34a-based therapeutic approaches for multiple myeloma treatment.

Scientific reports

Zarone MR,Misso G,Grimaldi A,Zappavigna S,Russo M,Amler E,Di Martino MT,Amodio N,Tagliaferri P,Tassone P,Caraglia M

Wed Dec 20 00:00:00 UTC 2017

Evidence of novel miR-34a-based therapeutic approaches for multiple myeloma treatment.

Scientific reports

Zarone MR,Misso G,Grimaldi A,Zappavigna S,Russo M,Amler E,Di Martino MT,Amodio N,Tagliaferri P,Tassone P,Caraglia M

Wed Dec 20 00:00:00 UTC 2017

Annexin A4 fucosylation enhances its interaction with the NF-kB p50 and promotes tumor progression of ovarian clear cell carcinoma.

Oncotarget

Wang H,Deng L,Cai M,Zhuang H,Zhu L,Hao Y,Gao J,Liu J,Li X,Lin B

Fri Dec 08 00:00:00 UTC 2017

Integrin endocytosis on elastic substrates mediates mechanosensing.

Journal of biomechanics

Li J,Chen H,Xu Y,Hu J,Xie FQ,Yang C

Tue Sep 06 00:00:00 UTC 2016

Integrin endocytosis on elastic substrates mediates mechanosensing.

Journal of biomechanics

Li J,Chen H,Xu Y,Hu J,Xie FQ,Yang C

Tue Sep 06 00:00:00 UTC 2016

Integrin endocytosis on elastic substrates mediates mechanosensing.

Journal of biomechanics

Li J,Chen H,Xu Y,Hu J,Xie FQ,Yang C

Tue Sep 06 00:00:00 UTC 2016

Integrin endocytosis on elastic substrates mediates mechanosensing.

Journal of biomechanics

Li J,Chen H,Xu Y,Hu J,Xie FQ,Yang C

Tue Sep 06 00:00:00 UTC 2016

CRISPR/Cas9n-Mediated Deletion of the Snail 1Gene (SNAI1) Reveals Its Role in Regulating Cell Morphology, Cell-Cell Interactions, and Gene Expression in Ovarian Cancer (RMG-1) Cells.

PloS one

Haraguchi M,Sato M,Ozawa M

Mon Apr 11 00:00:00 UTC 2016

CRISPR/Cas9n-Mediated Deletion of the Snail 1Gene (SNAI1) Reveals Its Role in Regulating Cell Morphology, Cell-Cell Interactions, and Gene Expression in Ovarian Cancer (RMG-1) Cells.

PloS one

Haraguchi M,Sato M,Ozawa M

Mon Apr 11 00:00:00 UTC 2016

Sine oculis homeobox homolog 1 promotes α5β1-mediated invasive migration and metastasis of cervical cancer cells.

Biochemical and biophysical research communications

Liu D,Zhang XX,Wan DY,Xi BX,Ma D,Wang H,Gao QL

Fri Apr 04 00:00:00 UTC 2014

Sine oculis homeobox homolog 1 promotes α5β1-mediated invasive migration and metastasis of cervical cancer cells.

Biochemical and biophysical research communications

Liu D,Zhang XX,Wan DY,Xi BX,Ma D,Wang H,Gao QL

Fri Apr 04 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: CD49 antigen-like family member E; CD49e; fibronectin receptor alpha polypeptide; Fibronectin receptor subunit alpha; fibronectin receptor, alpha polypeptide; fibronectin receptor, alpha subunit; integrin alpha 5; Integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); very late activation protein 5, alpha subunit; VLA-5

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Gene Aliases: CD49e; FNRA; ITGA5; VLA-5; VLA5; VLA5A

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UniProt ID: (Human) P08648, (Mouse) P11688

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Entrez Gene ID: (Human) 3678, (Mouse) 16402

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virus receptor activity epidermal growth factor receptor binding platelet-derived growth factor receptor binding integrin binding protein binding vascular endothelial growth factor receptor 2 binding metal ion binding cell adhesion molecule binding

cell-substrate junction assembly cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion integrin-mediated signaling pathway memory positive regulation of cell-substrate adhesion positive regulation of cell migration cell-substrate adhesion cell-cell adhesion mediated by integrin heterotypic cell-cell adhesion endodermal cell differentiation positive regulation of peptidyl-tyrosine phosphorylation negative regulation of anoikis angiogenesis extracellular matrix organization positive regulation of vascular endothelial growth factor receptor signaling pathway wound healing, spreading of epidermal cells viral entry into host cell leukocyte migration positive regulation of sprouting angiogenesis

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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