MUbio

Integrin beta 1D Monoclonal Antibody (1G2)

View all (2) Integrin beta 1d antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MUB0907P

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100-1:500
-

Immunohistochemistry (Frozen) (IHC (F))

1:50-1:100
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

1G2

Immunogen

Integrin beta 1D

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

1G2 recognizes specifically the cytoplasmic domain of integrin subunit beta-1D present in cardiac and skeletal muscle.

A broad species reactivity is expected because of the conserved nature of the epitope.

The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Target Information

Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated alpha and beta subunits. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migRation and apoptosis. There are two major forms of integrin beta 1: Beta 1A and Beta 1D, which differ in 13 amino acids. Their distribution pattern in adult tissues is mutually exclusive. Beta 1A is present in all tissues, except cardiac and skeletal muscle, which instead express the Beta 1D variant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD29; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; integrin beta 1; Integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); very late activation protein, beta polypeptide; VLA-4 subunit beta

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Gene Aliases: 4633401G24Rik; AA409975; AA960159; CD29; ENSMUSG00000051907; FNRB; Gm9863; GPIIA; ITGB1; MDF2; MSK12; VLA-BETA; VLAB

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UniProt ID: (Human) P05556, (Mouse) Q3TWH6

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Entrez Gene ID: (Human) 3688, (Mouse) 16412

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Function(s)
virus receptor activity glycoprotein binding fibronectin binding protease binding actin binding integrin binding protein binding coreceptor activity protein kinase binding protein domain specific binding C-X3-C chemokine binding protein complex binding peptide binding laminin binding metal ion binding protein heterodimerization activity cell adhesion molecule binding alpha-actinin binding collagen binding involved in cell-matrix adhesion cadherin binding involved in cell-cell adhesion receptor activity receptor binding collagen binding kinase binding
Process(es)
G1/S transition of mitotic cell cycle in utero embryonic development cell fate specification tissue homeostasis cell migration involved in sprouting angiogenesis cellular calcium ion homeostasis cellular defense response homophilic cell adhesion via plasma membrane adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion calcium-independent cell-matrix adhesion transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway regulation of G-protein coupled receptor protein signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation germ cell migration visual learning regulation of collagen catabolic process positive regulation of cell-substrate adhesion positive regulation of neuron projection development response to activity cell migration formation of radial glial scaffolds B cell differentiation extracellular matrix organization positive regulation of cell migration negative regulation of cell projection organization cell-substrate adhesion receptor internalization protein transport within lipid bilayer cell-cell adhesion mediated by integrin heterotypic cell-cell adhesion response to gonadotropin negative regulation of Rho protein signal transduction response to drug positive regulation of apoptotic process stress fiber assembly positive regulation of MAPK cascade positive regulation of GTPase activity sarcomere organization negative regulation of neuron differentiation positive regulation of endocytosis viral entry into host cell mesodermal cell differentiation axon extension dendrite morphogenesis positive regulation of peptidyl-tyrosine phosphorylation regulation of immune response leukocyte migration leukocyte tethering or rolling regulation of cell cycle cardiac muscle cell differentiation maternal process involved in female pregnancy bicellular tight junction assembly cellular response to mechanical stimulus cellular response to vitamin D cellular response to low-density lipoprotein particle stimulus cellular response to ionizing radiation positive regulation of establishment of protein localization to plasma membrane negative regulation of anoikis cell adhesion neuron projection development negative regulation of cell differentiation positive regulation of neuron differentiation cardiac muscle tissue development
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