Invitrogen

Integrin beta 5 Monoclonal Antibody (KN52), eBioscience™

3 Published Figures

15 References

View all (17) ITGB5 (Integrin beta 5) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (3)

Integrin beta 5 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 4

Integrin beta 5 Antibody (14-0497-82) in Flow

Staining of Chinese Hamster Ovary (CHO) cell line with 0.25 µg of Mouse IgG1kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Integrin beta 5 Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Total viable cells were used for analysis.

Integrin beta 5 Antibody in Immunocytochemistry (ICC/IF)

Integrin beta 5 Antibody in Neutralization (Neu)

Product Image Gallery

Product Details

14-0497-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 4 publications 4 publications

Neutralization (Neu)

View 2 publications 2 publications

Functional Assay (Functional)

View 3 publications 3 publications

Inhibition Assays (IA)

View 2 publications 2 publications

Blocking Assay (BLOCK)

View 1 publication 1 publication

Affinity Purification (AP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Hamster, Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

KN52

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_467288

Product Specific Information

Description: The KN52 antibody reacts with human integrin beta 5, an approximately 100,000 Mr (reduced) and 95,000 Mr (nonreduced) a member of the integrin family. Only a single alpha chain, the alpha v subunit, associates with the integrin beta 5 to form the vitronectin receptor complex. Integrin alpha v/beta 5 complex also binds to the basic domain of Tat (he sequence RKKRRQRRR). The integrin beta 5 is found on carcinoma cell lines, hepatoma and fibroblast cell lines, and is absent from lymphoblastoid cells and platelets.

The KN52 monoclonal antibody has been shown to inhibit migration.

Applications Reported: This KN52 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This KN52 antibody has been tested by flow cytometric analysis of Chinese Hamster Ovary (CHO), A549 and normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around 22 different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell-surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in-groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N-terminal extracellular domain that binds the ECM ligand and a small C-terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Integrin αvβ3 Is a Master Regulator of Resistance to TKI-Induced Ferroptosis in HER2-Positive Breast Cancer.

Cancers

Nagpal A,Needham K,Lane DJR,Ayton S,Redvers RP,John M,Selistre-de-Araujo HS,Denoyer D,Pouliot N

14-0497-82 was used in Flow cytometry/Cell sorting to provide critical insights into new therapeutic strategies to improve the treatment of advanced HER2-positive breast cancer patients.

Tue Feb 14 00:00:00 UTC 2023

Melanoma-Secreted Amyloid Beta Suppresses Neuroinflammation and Promotes Brain Metastasis.

Cancer discovery

Kleffman K,Levinson G,Rose IVL,Blumenberg LM,Shadaloey SAA,Dhabaria A,Wong E,Galán-Echevarría F,Karz A,Argibay D,Von Itter R,Floristán A,Baptiste G,Eskow NM,Tranos JA,Chen J,Vega Y Saenz de Miera EC,Call M,Rogers R,Jour G,Wadghiri YZ,Osman I,Li YM,Mathews P,DeMattos RB,Ueberheide B,Ruggles KV,Liddelow SA,Schneider RJ,Hernando E

14-0497-82 was used in Immunohistochemistry to reveal a novel mechanistic connection between brain metastasis and Alzheimer's disease, two previously unrelated pathologies; establish Aβ as a promising therapeutic target for brain metastasis; and demonstrate suppression of neuroinflammation as a critical feature of metastatic adaptation to the brain parenchyma.

Mon May 02 00:00:00 UTC 2022

Oxidized/deamidated-ceruloplasmin dysregulates choroid plexus epithelial cells functionality and barrier properties via RGD-recognizing integrin binding.

Neurobiology of disease

Zanardi A,Barbariga M,Conti A,Vegliani F,Curnis F,Alessio M

14-0497-82 was used in Cell adhesion to investigate the effects that oxidized/deamidated ceruloplasmin (Cp-ox/de) may exert on Choroid plexus epithelial cells (CPEpiCs) functions.

Fri Oct 01 00:00:00 UTC 2021

Blood vitronectin is a major activator of LIF and IL-6 in the brain through integrin-FAK and uPAR signaling.

Journal of cell science

Keasey MP,Jia C,Pimentel LF,Sante RR,Lovins C,Hagg T

14-0497-82 was used in In vivo experiments to propose that blood-derived vitronectin leakage into tissues promotes inflammation, indicating that it is a novel IL-6 and LIF regulation mechanism.

Fri Feb 02 00:00:00 UTC 2018

Diverse Requirements for Microglial Survival, Specification, and Function Revealed by Defined-Medium Cultures.

Neuron

Bohlen CJ,Bennett FC,Tucker AF,Collins HY,Mulinyawe SB,Barres BA

14-0497 was used in cell sorting to indicate that the specialized gene expression profile of mature microglia requires continuous instructive signaling from the intact CNS.

Wed May 17 00:00:00 UTC 2017

A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.

The Journal of general physiology

Wolfes AC,Ahmed S,Awasthi A,Stahlberg MA,Rajput A,Magruder DS,Bonn S,Dean C

14049782 was used in blocking or activating experiment to develop methods to study vesicle-associated proteins and exocytosis in stellate astrocytes

Sun Jan 01 00:00:00 UTC 2017

Complex I assembly into supercomplexes determines differential mitochondrial ROS production in neurons and astrocytes.

Proceedings of the National Academy of Sciences of the United States of America

Lopez-Fabuel I,Le Douce J,Logan A,James AM,Bonvento G,Murphy MP,Almeida A,Bolaños JP

Published figure using Integrin beta 5 monoclonal antibody (Product # 14-0497-82) in Flow Cytometry

Tue Nov 15 00:00:00 UTC 2016

Astrocyte NMDA receptors' activity sustains neuronal survival through a Cdk5-Nrf2 pathway.

Cell death and differentiation

Jimenez-Blasco D,Santofimia-Castaño P,Gonzalez A,Almeida A,Bolaños JP

14049782 was used in flow cytometry to study the Cdk5-Nrf2 pathway and its implication in neuronal protection against ROS.

Sun Nov 01 00:00:00 UTC 2015

Astrocyte NMDA receptors' activity sustains neuronal survival through a Cdk5-Nrf2 pathway.

Cell death and differentiation

Jimenez-Blasco D,Santofimia-Castaño P,Gonzalez A,Almeida A,Bolaños JP

14049782 was used in flow cytometry to study the Cdk5-Nrf2 pathway and its implication in neuronal protection against ROS.

Sun Nov 01 00:00:00 UTC 2015

The matricellular protein CCN1 mediates neutrophil efferocytosis in cutaneous wound healing.

Nature communications

Jun JI,Kim KH,Lau LF

14-0497 was used in Blocking experiments to establish the matricellular protein, CCN1, as a critical opsonin in skin injury.

Tue Jun 16 00:00:00 UTC 2015

Recombinant fibronectin matrix mimetics specify integrin adhesion and extracellular matrix assembly.

Tissue engineering. Part A

Roy DC,Hocking DC

14-0497 was used in Blocking experiments to analyze fibronectin matrix mimetic variants designed to include all or part of the integrin-binding domain for their ability to support new ECM assembly.

Fri Feb 01 00:00:00 UTC 2013

Inhibition of a novel specific neuroglial integrin signaling pathway increases STAT3-mediated CNTF expression.

Cell communication and signaling : CCS

Keasey MP,Kang SS,Lovins C,Hagg T

Published figure using Integrin beta 5 monoclonal antibody (Product # 14-0497-82) in Neutralization

Tue May 21 00:00:00 UTC 2013

Development of a method for the purification and culture of rodent astrocytes.

Neuron

Foo LC,Allen NJ,Bushong EA,Ventura PB,Chung WS,Zhou L,Cahoy JD,Daneman R,Zong H,Ellisman MH,Barres BA

Published figure using Integrin beta 5 monoclonal antibody (Product # 14-0497-82) in Immunocytochemistry

Thu Sep 08 00:00:00 UTC 2011

Macrophage-derived SPARC bridges tumor cell-extracellular matrix interactions toward metastasis.

Cancer research

Sangaletti S,Di Carlo E,Gariboldi S,Miotti S,Cappetti B,Parenza M,Rumio C,Brekken RA,Chiodoni C,Colombo MP

Sat Nov 01 00:00:00 UTC 2008

Homing of mouse spermatogonial stem cells to germline niche depends on beta1-integrin.

Cell stem cell

Kanatsu-Shinohara M,Takehashi M,Takashima S,Lee J,Morimoto H,Chuma S,Raducanu A,Nakatsuji N,Fässler R,Shinohara T

14-0497 was used in Inhibition experiments to identify beta1-integrin as an essential adhesion receptor for spermatogonial stem cell homing in mice.

Thu Nov 06 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: CD61; FLJ26658; GP3A; GPIIIa; integrin beta 5; Integrin beta-5; integrin, beta 5; testis secretory sperm-binding protein Li 217p

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Gene Aliases: [b]-5; [b]5; [b]5A; [b]5B; AA475909; AI874634; beta-5; beta5; ESTM23; I79_000655; ITGB5

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UniProt ID: (Human) P18084, (Mouse) O70309

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Entrez Gene ID: (Human) 3693, (Mouse) 16419, (Chinese hamster) 100765378

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Function(s)

virus receptor activity protein binding receptor activity receptor binding integrin binding

Process(es)

cell adhesion cell-matrix adhesion transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway stress fiber assembly epithelial cell-cell adhesion antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent muscle contraction extracellular matrix organization endodermal cell differentiation viral entry into host cell

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Integrin beta 5 Monoclonal Antibody (KN52), eBioscience™

Integrin beta 5 Antibody (14-0497-82) in Flow

Product Details

14-0497-82

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Inhibition Assays (IA)

Blocking Assay (BLOCK)

Affinity Purification (AP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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