Invitrogen

JAK2 Monoclonal Antibody (691R5)

18 Published Figures

16 References

View all (72) JAK2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (18)

FIGURE: 1 / 19

JAK2 Antibody (AHO1352) in WB

Proteins from cell extract of human Jurkat cells were resolved by SDS-PAGE and transferred to PVDF. The membranes were incubated with this JAK2 monoclonal antibody (clone 691R5) at a concentration of 1 µg/mL for two hours at room temperature.

JAK2 Antibody in Immunohistochemistry (IHC)

JAK2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

AHO1352

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 3 publications 3 publications

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

691R5

Immunogen

Recombinant fragment of human JAK2 expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2536334

Target Information

Janus kinase 2 (JAK2) is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways and is mutated in polycythemia vera. This kinase contains the catalytic domain (JH1) of JAK2. The activation of JAKs can lead to the phosphorylation of STAT (signal transducers and activatiors of transcription) proteins, which dimerize and translocate to the nucleus. Once translocated to the nucleus, the STAT proteins can modify transcription of numerous genes, including interferon-stimulated genes. JAK2 is required for the IFN gamma-receptor complex initiation and JAK1 functions as an amplifier. Some studies have suggested that the role of JAK2 might be performed by Tyk2 and JAK3, if they were positioned correctly within the IFN gamma-receptor complex. Mutations affecting JAK2 can cause Budd-Chiari syndrome, Polycythemia vera, Thrombocythemia 3, myelofibrosis, or Acute myelogenous leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Abi1 mediates airway smooth muscle cell proliferation and airway remodeling via Jak2/STAT3 signaling.

iScience

Wang R,Wang Y,Liao G,Chen B,Panettieri RA,Penn RB,Tang DD

AHO1352 was used in Immunocytochemistry to identify Abi1 as a molecular switch that activates Jak2 kinase and STAT3 in ASM cells and demonstrate that a dysfunctional Abi1-associated pathway contributes to the progression of asthma.

Fri Feb 18 00:00:00 UTC 2022

The activity of glyoxylase 1 is regulated by glucose-responsive phosphorylation on Tyr136.

Molecular metabolism

Cortizo FG,Pfaff D,Wirth A,Schlotterer A,Medert R,Morgenstern J,Weber T,Hammes HP,Fleming T,Nawroth PP,Freichel M,Teleman AA

AHO1352 was used in Western Blotting to suggest the existence of a deleterious positive feedback loop whereby hyperglycemia leads to reduced Glo1 activity, contributing to elevated MG levels, which in turn promote hyperglycemia.

Sat Jan 01 00:00:00 UTC 2022

CEP55 Promotes Cell Motility via JAK2⁻STAT3⁻MMPs Cascade in Hepatocellular Carcinoma.

Cells

Li M,Gao J,Li D,Yin Y

AHO1352 was used in Western Blotting to study the mechanisms by which CEP55 promotes hepatocellular carcinoma cell migration and invasion.

Wed Aug 08 00:00:00 UTC 2018

CEP55 Promotes Cell Motility via JAK2⁻STAT3⁻MMPs Cascade in Hepatocellular Carcinoma.

Cells

Li M,Gao J,Li D,Yin Y

AHO1352 was used in Western Blotting to study the mechanisms by which CEP55 promotes hepatocellular carcinoma cell migration and invasion.

Wed Aug 08 00:00:00 UTC 2018

Fos-Zippered GH Receptor Cytosolic Tails Act as Jak2 Substrates and Signal Transducers.

Molecular endocrinology (Baltimore, Md.)

Nespital T,van der Velden LM,Mensinga A,van der Vaart ED,Strous GJ

AHO1352 was used in western blot to study behavior of Fos-zippered GH receptor cytosolic tails that act as signal transducers and Jak2 substrates

Tue Mar 01 00:00:00 UTC 2016

Fos-Zippered GH Receptor Cytosolic Tails Act as Jak2 Substrates and Signal Transducers.

Molecular endocrinology (Baltimore, Md.)

Nespital T,van der Velden LM,Mensinga A,van der Vaart ED,Strous GJ

AHO1352 was used in western blot to study behavior of Fos-zippered GH receptor cytosolic tails that act as signal transducers and Jak2 substrates

Tue Mar 01 00:00:00 UTC 2016

Tyrosyl phosphorylated PAK1 regulates breast cancer cell motility in response to prolactin through filamin A.

Molecular endocrinology (Baltimore, Md.)

Hammer A,Rider L,Oladimeji P,Cook L,Li Q,Mattingly RR,Diakonova M

Published figure using JAK2 monoclonal antibody (Product # AHO1352) in Western Blot

Fri Mar 01 00:00:00 UTC 2013

The Jak/STAT signaling pathway is downregulated at febrile temperatures.

PloS one

Nespital T,Strous GJ

AHO1352 was used in western blot to discuss the functions of the Janus family of kinases.

Mon May 06 00:00:00 UTC 2013

Jak2 is a negative regulator of ubiquitin-dependent endocytosis of the growth hormone receptor.

PloS one

Putters J,da Silva Almeida AC,van Kerkhof P,van Rossum AG,Gracanin A,Strous GJ

AHO1352 was used in immunoprecipitation and western blot to discuss regulation of cytokine receptors at the cell surface.

Wed Feb 09 00:00:00 UTC 2011

Jak2 is a negative regulator of ubiquitin-dependent endocytosis of the growth hormone receptor.

PloS one

Putters J,da Silva Almeida AC,van Kerkhof P,van Rossum AG,Gracanin A,Strous GJ

AHO1352 was used in immunoprecipitation and western blot to discuss regulation of cytokine receptors at the cell surface.

Wed Feb 09 00:00:00 UTC 2011

PAK1-Nck regulates cyclin D1 promoter activity in response to prolactin.

Molecular endocrinology (Baltimore, Md.)

Tao J,Oladimeji P,Rider L,Diakonova M

AHO1352 was used in immunocytochemistry to study PAK1-dependent mechanisms that activate cyclin D1 promoter activity in response to prolactin.

Thu Sep 01 00:00:00 UTC 2011

Janus kinase 2 is required for the initiation but not maintenance of prolactin-induced mammary cancer.

Oncogene

Sakamoto K,Triplett AA,Schuler LA,Wagner KU

AHO1352 was used in western blot to explore the contribution of Jak2/Stat5 signaling during mammary cancer initiation and progression

Thu Sep 30 00:00:00 UTC 2010

Enhanced expression of Janus kinase-signal transducer and activator of transcription pathway members in human diabetic nephropathy.

Diabetes

Berthier CC,Zhang H,Schin M,Henger A,Nelson RG,Yee B,Boucherot A,Neusser MA,Cohen CD,Carter-Su C,Argetsinger LS,Rastaldi MP,Brosius FC,Kretzler M

AHO1352 was used in western blot to determine the expression of genes in glomeruli and tubulointerstitium in kidney biopsies from diabetic nephropathy patients.

Sun Feb 01 00:00:00 UTC 2009

Enhanced expression of Janus kinase-signal transducer and activator of transcription pathway members in human diabetic nephropathy.

Diabetes

Berthier CC,Zhang H,Schin M,Henger A,Nelson RG,Yee B,Boucherot A,Neusser MA,Cohen CD,Carter-Su C,Argetsinger LS,Rastaldi MP,Brosius FC,Kretzler M

AHO1352 was used in western blot to determine the expression of genes in glomeruli and tubulointerstitium in kidney biopsies from diabetic nephropathy patients.

Sun Feb 01 00:00:00 UTC 2009

Adapter protein SH2B1beta cross-links actin filaments and regulates actin cytoskeleton.

Molecular endocrinology (Baltimore, Md.)

Rider L,Tao J,Snyder S,Brinley B,Lu J,Diakonova M

Wed Jul 01 00:00:00 UTC 2009

Adapter protein SH2B1beta cross-links actin filaments and regulates actin cytoskeleton.

Molecular endocrinology (Baltimore, Md.)

Rider L,Tao J,Snyder S,Brinley B,Lu J,Diakonova M

Wed Jul 01 00:00:00 UTC 2009

JAK2 tyrosine kinase phosphorylates PAK1 and regulates PAK1 activity and functions.

The Journal of biological chemistry

Rider L,Shatrova A,Feener EP,Webb L,Diakonova M

AHO1352 was used in western blot to elucidate the role of tyrosyl phosphorylation in the regulation of PAK1 function

Fri Oct 19 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: EC 2.7.10.2; JAK 2; JAK-2; Janus kinase 2; Janus kinase 2 (a protein tyrosine kinase); JTK 10; kinase Jak2; Tyrosine-protein kinase JAK2

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Gene Aliases: Fd17; JAK2; JTK10; THCYT3

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UniProt ID: (Human) O60674, (Rat) Q62689, (Mouse) Q62120

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Entrez Gene ID: (Human) 3717, (Rat) 24514, (Mouse) 16452

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Function(s)

protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity receptor binding growth hormone receptor binding interleukin-12 receptor binding protein binding ATP binding protein C-terminus binding protein kinase binding heme binding type 1 angiotensin receptor binding acetylcholine receptor binding histone kinase activity (H3-Y41 specific) SH2 domain binding histone binding phosphatidylinositol 3-kinase binding insulin receptor substrate binding metal ion binding peptide hormone receptor binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

Performance Guarantee

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JAK2 Monoclonal Antibody (691R5)

JAK2 Antibody (AHO1352) in WB

Product Details

AHO1352

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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