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Invitrogen
JAML Monoclonal Antibody (eBio4E10 (4E10)), eBioscience™
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FIGURE: 1 / 4
JAML Antibody (14-5666-82) in Flow
Staining of mouse JAML-transfected CHO cells with 0.5 µg of Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) or 0.5 µg of Anti-Mouse JAML Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC (Product # 11-4111). Total viable cells were used for analysis.
Product Details
14-5666-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
eBio4E10 (4E10)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_795882
Product Specific Information
Description: The eBio4E10 monoclonal antibody reacts with mouse JAML (junctional adhesion molecule-like protein), also known as AMICA. JAML is a glycosylated membrane protein possessing two Ig-like domains in its extracellular domain. JAML is expressed on polymorphonuclear leukocytes (PMN), and is involved in the transmigration of such cells through epithelial tight junctions via interactions between JAML and epithelial coxsackie and adenovirus receptor (CAR). JAML is also expressed on lymphocyte subsets, such as gammadelta T cell lines, and can provide co-stimulatory signals for cell activation.
Applications Reported: This eBio4E10 antibody has been reported for use in flow cytometric analysis and immunoprecipitation.
Applications Tested: This eBio4E10 (4E10) antibody has been tested by flow cytometric analysis of mouse lysed whole blood. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
AMICA transmembrane protein of the plasma membrane of leukocytes that control their migration and activation through interaction with CXADR, a plasma membrane receptor found on adjacent epithelial and endothelial cells. The interaction between both receptors mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. It also controls the transmigration of leukocytes within epithelial and endothelial tissues through adhesive interactions with epithelial and endothelial CXADR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: adhesion molecule AMICA; Dendritic cell-specific protein CREA7; dendritic-cell specific protein Crea7; Junctional adhesion molecule-like; mCrea7
Gene Aliases: AMICA; Amica1; Crea7; Gm638; Jaml
UniProt ID: (Mouse) Q80UL9
Entrez Gene ID: (Mouse) 270152
immune system process
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
neutrophil chemotaxis
monocyte extravasation
gamma-delta T cell activation
leukocyte migration
positive regulation of epithelial cell proliferation involved in wound healing
neutrophil extravasation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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