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Invitrogen
JHDM1D Polyclonal Antibody
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JHDM1D Antibody (PA5-89986) in WB
Western blot analysis of JHDM1D in T-47D cells. Samples were incubated with JHDM1D Polyclonal antibody (Product # PA5-89986) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-89986
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of mouse KDM7A (NP_0010286022)
Conjugate
Form
Liquid
Concentration
0.34 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805876
Product Specific Information
Immunogen sequence: WHRHDYTEVD DGSKPVQAGT RAFVKELRSR VFPSADEIIV KMHGSQLTQR YLEKHGFDVP IMVPKLDDLG LRLPSPAFSV MDVERYVGGD KVIDVIDVAR Q
Target Information
JHDM1D histone demethylase that specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3, thereby playing a central role in histone code. This specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. This demethylates H3K9me2 in absence of H3K4me3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: [histone H3]-dimethyl-L-lysine9 demethylase 7A; histone lysine demethylase JHDM1D; JmjC domain-containing histone demethylation protein 1D; jumonji C domain containing histone demethylase 1 homolog D; jumonji C domain-containing histone demethylase 1 homolog D; lysine (K)-specific demethylase 7A; Lysine-specific demethylase 7; Lysine-specific demethylase 7A
Gene Aliases: A630082K20Rik; BB041802; ENSMUSG00000073143; JHDM1D; KDM7; KDM7A; KIAA1718; mKIAA1718
UniProt ID: (Human) Q6ZMT4, (Mouse) Q3UWM4
Entrez Gene ID: (Human) 80853, (Mouse) 338523
iron ion binding
zinc ion binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
histone demethylase activity
histone demethylase activity (H3-K9 specific)
methylated histone binding
histone demethylase activity (H4-K20 specific)
histone demethylase activity (H3-K36 specific)
histone demethylase activity (H3-K27 specific)
sulfonate dioxygenase activity
oxidoreductase activity
2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity
procollagen-proline dioxygenase activity
hypophosphite dioxygenase activity
DNA-N1-methyladenine dioxygenase activity
metal ion binding
dioxygenase activity
C-19 gibberellin 2-beta-dioxygenase activity
C-20 gibberellin 2-beta-dioxygenase activity
transcription, DNA-templated
midbrain development
histone H3-K9 demethylation
histone H4-K20 demethylation
positive regulation of transcription, DNA-templated
oxidation-reduction process
histone H3-K36 demethylation
histone H3-K27 demethylation
regulation of transcription, DNA-templated
nervous system development
chromatin modification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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