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Invitrogen
JMY Polyclonal Antibody
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Product Details
PA5-143713
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
JMY synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of mouse JMY. This sequence is highly conserved in human, rat, frog, and zebrafish JMY proteins, and has low homology to other WASP family members.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2942941
Product Specific Information
The antibody detects a 130 kDa* protein corresponding to the molecular mass of JMY on SDS-PAGE immunoblots of human HeLa, Jurkat cells, rat PC12 cells, and mouse brain and heart.
Target Information
JMY acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ37870; Junction-mediating and -regulatory protein; junction-mediating and regulatory protein; p300 transcriptional cofactor JMY; similar to junction-mediating and regulatory protein; WAS protein homology region 2 domain containing 1-like 3
Gene Aliases: JMY; WHAMM2; WHDC1L3
UniProt ID: (Human) Q8N9B5, (Mouse) Q9QXM1
Entrez Gene ID: (Human) 133746, (Mouse) 57748, (Rat) 683593
DNA repair
regulation of transcription from RNA polymerase II promoter
cell cycle arrest
Arp2/3 complex-mediated actin nucleation
positive regulation of apoptotic process
positive regulation of sequence-specific DNA binding transcription factor activity
'de novo' actin filament nucleation
actin polymerization-dependent cell motility
regulation of signal transduction by p53 class mediator
cellular response to DNA damage stimulus
regulation of DNA-templated transcription in response to stress
intrinsic apoptotic signaling pathway by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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