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Invitrogen
Jagged 2 Monoclonal Antibody (HMJ2-1), eFluor™ 660, eBioscience™
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Product Details
50-3392-82
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HMJ2-1
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574203
Product Specific Information
Description: This HMJ2-1 monoclonal antibody reacts with mouse Jagged-2, one of five type I transmembrane proteins that serves as a Notch receptor ligand. Upon binding the Notch receptor (e.g., Notch 1-4), Jagged-2 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases an intracellular fragment whose biological function remains controversial. Jagged-2 is expressed by thymic lymphoid and stromal cells, as well as macrophages and dendritic cells in the spleen.
Note: Staining of endogenous splenic dendritic (CD8+CD11c+) cells could only be consistently confirmed in cells isolated from C57BL/6, but not Balb/c, mice. Other strains have not been tested. The antiobdy has been reported to crossreact to rat and have blocking function.
Applications Reported: This HMJ2-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HMJ2-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. JAG2 is one of several ligands that activate Notch and related receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: Jagged2; Protein jagged-2; syndactylism
Gene Aliases: D12Ggc2e; Jag2; mJagged2-1; Serh; sm
UniProt ID: (Mouse) Q9QYE5
Entrez Gene ID: (Mouse) 16450, (Rat) 29147
skeletal system development
in utero embryonic development
cell fate determination
respiratory system process
cell communication
Notch signaling pathway
multicellular organism development
spermatogenesis
sensory perception of sound
auditory receptor cell fate commitment
morphogenesis of embryonic epithelium
cell differentiation
regulation of cell adhesion
T cell differentiation
regulation of cell proliferation
odontogenesis of dentin-containing tooth
gamma-delta T cell differentiation
thymic T cell selection
epithelial cell apoptotic process involved in palatal shelf morphogenesis
response to hypoxia
brain development
male gonad development
embryonic limb morphogenesis
regulation of cell differentiation
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