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KAP-1 Antibody (A304-144A) in WB
Detection of mouse KAP-1 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 cells treated with 100 µM etoposide for 2 hours (+) or mock treated (-) cells, NIH 3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody (Product # A304-144A) (lot A304-144A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A304-144A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 175 to 225 of mouse Tripartite Motif-Containing 28.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat
Target Information
The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome. These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1). KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression. KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression. Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 SUMO-protein ligase TRIM28; KRAB-A-interacting protein; KRAB-interacting p; KRIP-1; RING-type E3 ubiquitin transferase TIF1-beta; tif1 beta; TIF1-beta; Transcription intermediary factor 1-beta; transcriptional intermediary factor 1, beta; tripartite motif; tripartite motif protein 28; Tripartite motif-containing protein 28
Gene Aliases: AA408787; KAP-1; Kap1; KRIP-1; Krip1; MommeD9; Tif1b; Tif1beta; Trim28
UniProt ID: (Mouse) Q62318
Entrez Gene ID: (Mouse) 21849
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
transcription corepressor activity
protein kinase activity
ubiquitin-protein transferase activity
protein binding
zinc ion binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
ubiquitin protein ligase binding
Krueppel-associated box domain binding
sequence-specific DNA binding
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
poly(A) RNA binding
metal ion binding
chromo shadow domain binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
epithelial to mesenchymal transition
DNA repair
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
embryo implantation
protein ubiquitination
protein sumoylation
positive regulation of transcription factor import into nucleus
DNA methylation involved in embryo development
innate immune response
positive regulation of DNA repair
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
protein autophosphorylation
protein oligomerization
convergent extension involved in axis elongation
embryonic placenta morphogenesis
negative regulation of DNA demethylation
negative regulation of viral release from host cell
regulation of genetic imprinting
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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