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KCNJ12 Antibody (OSK00028G-500UG) in WB
Rabbit antibody to cytoplasmic domain of KCNJ12 (KCNJN1, Kir2.2, IRK2): whole serum (OSK00021W) at a concentration of 30 µg/mL; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.
Product Details
OSK00028G-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from the cytoplasmic domain of rat KCNJ12 (KCNJN1, Kir2.2, IRK2) conjugated to an immunogenic carrier protein was used as the immunogen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
Ammonium sulfate precipitation
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
The antigen is homologous in mouse.
Specificity of this antibody: KCNJ12.
Target Information
Inward rectifying potassium channel that is activated by phosphatidylinositol 4,5-bisphosphate and that probably participates in controlling the resting membrane potential in electrically excitable cells. Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATP-sensitive inward rectifier potassium channel 12; Inward rectifier K(+) channel Kir2.2; IRK-2; Potassium channel, inwardly rectifying subfamily J member 12; potassium inwardly-rectifying channel, subfamily J, member 12
Gene Aliases: IRK-2; IRK2; Kcnj12; Kir2.1; Kir2.2; MB-IRK2
UniProt ID: (Mouse) P52187
Entrez Gene ID: (Mouse) 16515, (Rat) 117052
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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