Invitrogen

KCNQ3 Polyclonal Antibody

KCNQ3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 1

KCNQ3 Antibody (PA1-930) in ICC/IF

Immunofluorescence analysis of KCNQ3 was performed using 70% confluent log phase RSC-96 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with KCNQ3 Rabbit Polyclonal Antibody (Product # PA1-930) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More

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Product Details

PA1-930

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

0.5 µg/mL

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GST fusion protein encoding the first 71 amino acids of rat KCNQ3.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2131708

Product Specific Information

PA1-930 detects KCNQ3 from mouse, human and rat samples. This antibody is specific for KCNQ3 and does not detect KCNQ1, KCNQ2, KCNQ4 or KCNQ5.

PA1-930 has been successfully used in immunofluorescence, immunohistochemistry and immunocytochemistry procedures. Immunohistochemical staining using PA1-930 yielded a strong signal mainly in interneurons and astrocytes in the dentate region of rat hippocampal samples.

PA1-930 immunogen is a GST fusion protein encoding the first 71 amino acids of rat KCNQ3.

Target Information

KCNQs are members of the voltage-dependent non-inactivating potassium channel family. Currently there are five known KCNQs (KCNQ1-5) found in the central nervous system and KCNQ2 and 3 have demonstrated their importance in M-current activation. Studies have shown that KCNQ2 and KCNQ3 form heteromultimers that, when formed, substantially increase the M-current. Inhibition of M-current controls neuron excitability throughout the nervous system as well as the responsiveness to synaptic inputs. Genetic mutations in these proteins have been linked to disorders such as benign familial neonatal convulsions (BFNC), deafness, neuropathic pain and epilepsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nine patients with KCNQ2-related neonatal seizures and functional studies of two missense variants.

Scientific reports

Chokvithaya S,Caengprasath N,Buasong A,Jantasuwan S,Santawong K,Leela-Adisorn N,Tongkobpetch S,Ittiwut C,Saengow VE,Kamolvisit W,Boonsimma P,Bongsebandhu-Phubhakdi S,Shotelersuk V

PA1-930 was used in Immunocytochemistry to demonstrate that the surface membrane expression of Kv7.2 carrying either variant was decreased.

Mon Feb 27 00:00:00 UTC 2023

A novel homozygous KCNQ3 loss-of-function variant causes non-syndromic intellectual disability and neonatal-onset pharmacodependent epilepsy.

Epilepsia open

Lauritano A,Moutton S,Longobardi E,Tran Mau-Them F,Laudati G,Nappi P,Soldovieri MV,Ambrosino P,Cataldi M,Jouan T,Lehalle D,Maurey H,Philippe C,Miceli F,Vitobello A,Taglialatela M

PA1-930 was used in Western Blotting to indicate that a homozygous KCNQ3 loss-of-function variant is responsible for a severe phenotype characterized by neonatal-onset pharmacodependent seizures, with developmental delay and intellectual disability.

Sun Sep 01 00:00:00 UTC 2019

Loss of the m-AAA protease subunit AFG₃L₂ causes mitochondrial transport defects and tau hyperphosphorylation.

The EMBO journal

Kondadi AK,Wang S,Montagner S,Kladt N,Korwitz A,Martinelli P,Herholz D,Baker MJ,Schauss AC,Langer T,Rugarli EI

Published figure using KCNQ3 polyclonal antibody (Product # PA1-930) in Immunocytochemistry

Fri May 02 00:00:00 UTC 2014

Antibodies and a cysteine-modifying reagent show correspondence of M current in neurons to KCNQ2 and KCNQ3 K+ channels.

British journal of pharmacology

Roche JP,Westenbroek R,Sorom AJ,Hille B,Mackie K,Shapiro MS

PA1-930 was used in immunocytochemistry and immunohistochemistry to investigate the influence of KCNQ2 and KCNQ3 potassium channels on the M current of neurons

Sun Dec 01 00:00:00 UTC 2002

Bioinformatics

Protein Aliases: BFNC; KQT-like 3; Potassium channel subunit alpha KvLQT3; potassium channel, voltage gated KQT-like subfamily Q, member 3; potassium channel, voltage-gated, subfamily Q, member 3; Potassium voltage-gated channel subfamily KQT member 3; potassium voltage-gated channel, KQT-like subfamily, member 3; potassium voltage-gated channel, subfamily Q, member 3; Voltage-gated potassium channel subunit Kv7.3

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Gene Aliases: BFNC2; EBN2; KCNQ3; KV7.3

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UniProt ID: (Human) O43525, (Rat) O88944, (Mouse) Q8K3F6

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Entrez Gene ID: (Human) 3786, (Rat) 29682, (Mouse) 110862

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Function(s)

voltage-gated potassium channel activity delayed rectifier potassium channel activity potassium channel activity calmodulin binding protein kinase binding ubiquitin protein ligase binding ion channel binding ion channel activity voltage-gated ion channel activity

Process(es)

potassium ion transport chemical synaptic transmission regulation of ion transmembrane transport membrane hyperpolarization potassium ion transmembrane transport oligodendrocyte development ion transmembrane transport ganglion development cellular response to ammonium ion transport ion transport transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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KCNQ3 Polyclonal Antibody

KCNQ3 Antibody (PA1-930) in ICC/IF

Product Details

PA1-930

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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